🚀 CALIPERS v2 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berteroale and @florianjug group members!.

RT @fspasqualini: 🚀 CALIPERS v2 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet….

The tour-de-force @unipv was enabled by generous funding from @ERC_Research and @HorizonEU #UNLOOC and carried out with tools from @luxendo @NikonUSA @NikonItalia @Photometrics @Okolabgroup @ibidiCells @illumina @thermofisher @STEMCELLTech.

Everything is open-science here, links in the pre-print, but reach out if you don't want to wait for the final repositories to be updated.

And finally for regeneration: by tracking rare hiPSC-derived cardiomyocytes that are about to re-enter the cell cycle, we can distinguish through cell proliferation (useful in regeneration) from multinucleation and endoreplication (useful to assess maturation)! All the while, we

New light-sheet capture of 4-color hiPSC-cardioids reveals compaction before calcium transients initiation. 90 % exit cycle by d4.5 → maturation on cue. #CCAwarePhenotyping #MethodInACellLine

Safe-harbor genome editing ensures expression across differentiated lineages, and WTC-11 genetic background means full compatibility with the @AllenCellInst Cell Collection: the largest repository of GFP/RFP-based hiPSC reporter lines

WTC11-based hiPSCs reporter lines. We provide a 4-color CALIPERS hiPSC line with sensors for actin, calcium, and the cell cycle, as well as a blank canvas line to add your favorite sensors. CALIPERS is a #MethodInACellLine!

Phase-locked motility.Each nucleus gets a %-cell-cycle score that rides with its mask and trajectory. Compare shape or speed by cycle progress, not frame time. Watch HaCaTs sprint through physical space and cell cycle (CC) phases! #PhaseLockedMotility #CCAwarePhenotyping

Proposed solution: To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.

Problem: Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and.

RT @HenriquesLab: 🧑‍🔬📰🚀 #RxivMaker is here! A framework automating the creation of publication-ready scientific #preprints from simple Mark….

RT @HenriquesLab: It's here! ✨.The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with eSRRF analysis. From….

RT @HenriquesLab: 🔬👨‍💻📰 #SReD is out! .Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bia….

RT @czbiohub: 🚫 No dyes. No bleaching. 🔬 Just AI + label-free microscopy = vivid virtually stained images. New in @NatMachIntell: A deep le….

RT @florianjug: Come and work with us!.Super exciting and relevant projects!.Become an #AI pro and do something positive for the 🌍! 👩‍🎓….

RT @CrestOptics: 🎥 Live from #ELMI2025 at @EMBL Heidelberg! Step into our world of microscopy. 📍 Find us at booth #F28 🤝 Come connect with….

RT @BrianHartPR: Young professionals, when you have a question, the first question to ask yourself is "Can I Google it?" (or "Can I Grok it….

Elezioni @unipv @alereali77 #IoVotoAle #InsiemeSiPuò . Dopo conferenza di ateneo mi è tornato in mente questo video (che cita Marianne Williamson). “Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure.

RT @ERC_Research: 📣 Key 2024 developments at the European Research Council. We are pleased to share the release of our new #AnnualReport,….