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Eray Enustun, Ph.D. Profile
Eray Enustun, Ph.D.

@EnustunEray

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PhD from @ucsdbiosciences ‘23 - B.S. in ChemEng and B.S. in Mol Biology&Genetics @kocuniversity ‘16, MSc. @kocuniversity ‘18

San Diego, CA
Joined November 2019
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@EnustunEray
Eray Enustun, Ph.D.
1 year
My second “first author” paper is published by Nucleic Acids Research! We show how one of the proteins I found from the proteomics screen (Enustun et al., Nat Struct Mol Biol, 2023) is important for mRNA transfer from the phage nucleus and for their translation.
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@EnustunEray
Eray Enustun, Ph.D.
1 year
One of the best works I contributed led by one of the best scientists I know @iamamardeeep & @_KevinCorbett lab. Check out the amazing paper that explores molecular mechanism of PARIS immune system!.
@iamamardeeep
Amar Deep
1 year
Out today in @Nature! .I am thrilled to share our work on the PARIS immune system in its final published form. Huge thanks to my mentor, Kevin Corbett @_KevinCorbett, and my teammates. See the thread below in comment section for a quick summary!.
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@EnustunEray
Eray Enustun, Ph.D.
1 year
Another great paper from our team (led by Chase), which I’m the second author of, is now published by PNAS! We investigated the selective protein import mechanism to the phage nucleus and our findings provide a mechanistic model to the system!.
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@EnustunEray
Eray Enustun, Ph.D.
1 year
@Emy_nim @jina_leemon @ben_a_adler @bradyfcress Finally, I would like to thank my PIs @_KevinCorbett & Dr. Joe Pogliano for the guidance and their supports. I would like to acknowledge NIH & HHMI for their funding. I couldn't do this work without my labmates and collaborators mentioned above (+ @iamamardeeep & Yajie Gu). n/n.
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@EnustunEray
Eray Enustun, Ph.D.
1 year
@Emy_nim @jina_leemon @ben_a_adler @bradyfcress Relatedly, LC-MS/MS showed ChmC knockdown causes a global reduction in phage protein and effects more present for the putative virion structural proteins. We believe this is due to ChmC's role in the stages of infection where transcription occurs in the phage nucleus. 8/n
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@EnustunEray
Eray Enustun, Ph.D.
1 year
@Emy_nim @jina_leemon @ben_a_adler @bradyfcress To test our hypothesis if ChmC is responsible for transfer of the mRNA from the phage nucleus to cytoplasm, we performed mRNA-FISH (with which showed accumulation of the mRNAs of virion structural proteins in the phage nucleus. 7/n
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@EnustunEray
Eray Enustun, Ph.D.
1 year
To investigate further in vivo, we (@Emy_nim & @jina_leemon) collaborated with Nobel laureate Dr. Jennifer Doudna's team (@ben_a_adler @bradyfcress) and used their new CRISPR-iART. Knockdown of ChmC during infection impairs phage nucleus development and halts infection. 6/n
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@EnustunEray
Eray Enustun, Ph.D.
1 year
Next, we performed eCLIP (a method developed esteemed to pulldown ChmC during the phage infection to investigate in vivo mRNA bindings. Sequencing and analysis showed ChmC binds phage mRNAs and it is enriched near start codons. 5/n
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@EnustunEray
Eray Enustun, Ph.D.
1 year
In addition to Whirly domain (nucleic acid binding), C-terminus disordered region is N/G-rich and ChmC forms phase separation condensates with mRNA. This is very interesting because it is shown phase separation is also shown for eukaryotic nucleus components for mRNA export. 4/n
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@EnustunEray
Eray Enustun, Ph.D.
1 year
Our structural analysis of ChmC showed it has nucleic acid binding domains and it has surface exposed positively charged residues. ChmC has higher affinity to bind mRNA and when we mutated the charged residues, it lost its ability to bind to mRNA. 3/n
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@EnustunEray
Eray Enustun, Ph.D.
1 year
In this paper, we investigated the newly termed "ChmC" protein, which is the second highest expressed protein (after ChmA) that is not a "virion-structural protein" during the phage nucleus-forming jumbo phages and it localizes with the phage nucleus. 2/n
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@EnustunEray
Eray Enustun, Ph.D.
2 years
I’m not saying this because I am a part of this work, but this is an amazing work! . Led by amazingly smart & talented @iamamardeeep.
@iamamardeeep
Amar Deep
2 years
In this bioRxiv preprint, we (@_KevinCorbett ) present the architecture and activation mechanism of the PARIS antiviral defense system. Thanks to our co-authors, @Lisa_Liang13, @EnustunEray, and @PoglianoJoe, for their support in shedding light on this Anti-anti-defense system.🧵
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@EnustunEray
Eray Enustun, Ph.D.
2 years
RT @justinrmeyer: Our newest paper was published today, a capstone to our work on gain-of-function evolution, phage-bacterial interaction n….
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@EnustunEray
Eray Enustun, Ph.D.
2 years
Today marks the 100th founding year anniversary of the Turkish Republic 🇹🇷. Ataturk, the visionary founder, said: "Science is the true mentor" for a better world. He advocated for "world peace" by listening each other & cooperation. Today, these visions are still much needed.
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@EnustunEray
Eray Enustun, Ph.D.
2 years
RT @_KevinCorbett: I'm thrilled to share the latest work from my lab, showing that a bacterial anti-phage immune system encodes a ubiquitin….
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biorxiv.org
Ubiquitination and related pathways play crucial roles in protein homeostasis, signaling, and innate immunity[1][1]-[3][2]. In these pathways, an enzymatic cascade of E1, E2, and E3 proteins conjug...
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@EnustunEray
Eray Enustun, Ph.D.
2 years
RT @ben_a_adler: Fascinating biology on how the phage nucleus translocates its RNA!. For a deep dive, check out @EnustunEray 's thread 👇.
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@EnustunEray
Eray Enustun, Ph.D.
2 years
Finally, special shoutouts to my amazing collaborators: @Emy_nim , @jina_leemon , @iamamardeeep , @thevillalab, @doudna_lab , @yeo_lab.Sitao Zhang (Cleveland lab) & all of my past and current labmates from Pogliano lab and Corbett lab! Thank you all!!.
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@EnustunEray
Eray Enustun, Ph.D.
2 years
We believe this work shows the first jumbo phages' protein plays a role in mRNA export from the phage nucleus and translation. It is very exciting as this replication system is sensitive to RNA-targeting defense systems & future works can allow to engineer better phage therapies!.
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@EnustunEray
Eray Enustun, Ph.D.
2 years
Finally, when we performed TMT-tagged MS during infection, we saw the knockdown of ChmC caused global reduction of phage protein-levels compared to NT, which the fold-change is increased for later expressed proteins (30 minutes to 60 & 90 minutes post infection).
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@EnustunEray
Eray Enustun, Ph.D.
2 years
With Doudna Lab's recently available (CRISPRi-ART), we knockdown phage encoded ChmC during the infection, which reduced the efficiency of plaquing and the infections stayed at an earlier stage (when the mRNA starts to be transcribed from inside of the phage nucleus)!
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