So happy to share the amazing work of @CarrenoYugueros using #synbio engineered bacteria to sense #microbiome nutrient availability in the gut with beautiful microscopy. @IC_CSynBio @ImperialInfect & @TheCrick.

📢 A fully-funded PhD position for UK-home student📢. Want to apply #synbio to understand the gut #microbiome in disease? Join the Riglar lab for your PhD! @ImperialInfect & @theCrick. Email CV, cover letter and details of 2 refs by 6th Sept to apply.

Hey @gibbological, have you seen the @nytimes Spelling Bee today?

RT @ravinash_kk: Few more days to apply for the PDRA position closing end of Jan!.

📢PhD positions: last day to apply📢. 1x fully-funded for UK-home student Also option to sponsor int'l students for scholarship - incl Send CV incl past grades, cover letter and details of 2 refs by email.

RT @thelowlab: Exciting faculty opportunity to join our structural biology community at Imperial College, London!! We are recruiting an EM….

RT @ravinash_kk: 📢Phd position available 📢.@ImperialInfect, @imperialcollege.working on 3D printing of anaerobic bacterial communities. 🦠🖨….

To apply please send cover letter detailing why you are interested in the lab, CV and 2 references via email.

Want to apply #synbio to understand the gut #microbiome in disease? Join the Riglar lab for your PhD! 1x funded position for UK home students. Can also sponsor 1x intl student for scholarship. Apply by 8th Dec. Details👇and @ImperialInfect & @theCrick.

#synbio people - are there any examples of published results from public consultation on the idea of delivering synthetic engineered organisms/cells to patients in the clinic?.

RT @ProfTomEllis: Come be a PI with me. our continually-awesome Bioengineering Dept is hiring for up to 4 new faculty members, including….

We are very grateful to funding from @wellcometrust. And welcome any feedback from the community to further improve the work.

Along the way we learn a lot about testing pools of engineered bacteria in the mammalian gut. And simplify the process of library design and construction. This leaves us ready to look for unique biosensors from a wider range of bacterial sources for more diverse applications 8/n.

Does it work? Yes! With the platform we identify a new sensor to tetrathionate - an inflammatory biomarker. We also find a sensor that responds to citrate within the gut. 7/n

We add barcodes to each sensor during construction in a one-pot reaction. Its is cheap to construct and sequence, gives us flexibility to have varied sensors and ongoing revision of our library, and allows us to test sensor replicates for more robust results. 6/n

How does it work? When a biosensor fires in our engineered E. coli, the #synbio memory circuit switches on - causing resistance to an antibiotic. We identify interesting sensors from a pool of candidates using differential antibiotic resistance and sequencing 5/n

#synbio has delivered various 'memory' circuits that record info in the gut and report on it via stool sampling. We extend our work on one of these systems ( to now screen more diverse libraries of candidate sensors, more robustly, and for less $$. 4/n

The solution: The #microbiome has diverse ways to sense the gut, commonly via two component systems. Maybe we could use TCSs to sense disease, treatment efficacy, location, or other microbes in the gut. But how do we find the best ones to use from so many varied bacteria? 3/n.

The vision: we want to build 'smart probiotics' that sense fingerprints of health and disease in situ within the gut and respond as diagnostics or on-demand therapeutics. The problem: how do we discover more powerful biosensors in bacteria for #synbio applications? 2/n.

So proud to share the 1st Riglar lab paper. An amazing effort from @KlondikeClare @CarrenoYugueros @thenextofus & Mindy Chen @ImperialInfect @TheCrick! A discovery platform to identify inducible synthetic circuitry from varied microbial sources 1/n.