SWARM (Single-molecule Workflow for Analysing RNA Modifications) detects pseudouridine, m6A, and m5C on individual molecules from direct RNA nanopore signals (in both chemistries RNA002 and RNA004) https://t.co/iMg2TWDKAO see more on poster P2-396 at #rna2025 #RNA25 #RNAmods

Thanks to all co-authors, without whom this work would be impossible. @GuarnacciMarco @starstuffkarthi @pol2rna @TominAustralia @RippeiH and others.

dFORCE is directly applicable to mammalian tissue samples, and produces results consistent with cell culture, unlocking analysis of mRNA synthesis kinetics in vivo:

We present a revised, multi-modal, single-molecule timeline for mRNA synthesis where splicing catalysis is largely deferred to after transcription, m6A methylation and cleavage and polyadenylation.

Is rapid co-transcriptional splicing abundant? Does the exon-junction complex shape m6A topology? Does 3' end formation occur in spatial proximity to transcription? We answer all these questions and more using dFORCE, a direct pre-mRNA sequencing technology.

We describe the temporal organisation of central pre-mRNA processing events, including splicing, m6A modification and 3' end formation, during mRNA synthesis. Check out the preprint here!

it gets me fr

R2Dtool https://t.co/czxyDjWT94 for handling, integrating, and viualising isoform-resolved RNA feature data - led by @_AJSethi #bioinformatics #RNA #isoforms https://t.co/wPGGZxHarv

Now online! Capturing totipotency in human cells through spliceosomal repression

It's finally published. This one took a long time to get through reviewers: Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications

Jonas Salk, inventor of polio vaccine: "There is no patent. Could you patent the sun?" Frederick Banting, inventor of insulin: "Insulin does not belong to me. It belongs to the world." Moderna CEO Stéphane Bancel: 400% price hike on COVID vaccine is "consistent with the value."

Kicking off a week of incredible Australian bioinformatics research with the COMBINE 2022 Symposium! @combine_au @abacbs #COMBINE22

🪐 Introducing Galactica. A large language model for science. Can summarize academic literature, solve math problems, generate Wiki articles, write scientific code, annotate molecules and proteins, and more. Explore and get weights: https://t.co/jKEP8S7Yfl

Did you know #PhD students in Australian #academia, people with a professional level of education, live ~$80 a week below the poverty line? PhD students account for about 33% of #academic output. Maybe it's time we unionised.

Simultaneous detection of m6A and m5C on individual RNA molecules using CHEUI (CH3 Estimation Using Ionic current). Thanks to everyone who helped!! @_AJSethi @EduEyras @GuarnacciMarco @tominaustralia ...etc #RNAmodifications #RNA #DeepLearning https://t.co/yFEFlstfek

A meeting not to be missed! Join us for the inaugural A-RNA meeting 15-18th May, amazing speaker line up and most of all, an oppurtunity be amongst the cool gang who love the other nucleic acid @ARNAconference #RNA

Submitting my work to nature… #academictwitter #phdchat #lornegenome ⁦@GenomeConf⁩

Can't belive in-person conferences are back!!! Road trip to @GenomeConf with @renzobalb and @_AJSethi!!

genetic blockchain

And that's a wrap for #COMBINE21! Thanks to my incredible co-chair @sachintha_wije & our committee, our speakers, career panelists, Prof. @AliciaOshlack, and finally, all our attendees. Proudly supported by @PawseyCentre @IntersectAust @MelBioInf @SA_genomics. Onto #ABACBS2021!!