nor require differential panels or dilutions! This tuning strategy with PLA highlights the opportunity to achieve sensitive, accurate quantification of diverse analytes in a highly multiplexed fashion. We're excited to see where this tuning is taken for better health monitoring!

We propose a tuning approach for proximity-based assays, enabling simultaneous quantification of four analytes across from low fM to mid-nM. Importantly, this solution does not require modification of the intrinsic affinity of affinity reagents to their targets... 3/4

The human plasma proteome spans over 10 orders of magnitude, but current quantification methods cover only 3-4 orders, greatly limiting what can be measured in a single panel and small sample volume. 2/4

New paper w/ @HTSohLab! This is a large part of my PhD, Im excited to share some of those results! In this work, we provide a strategy to overcome the problem of measuring proteins that span >7 orders of magnitude using only 5ul of serum. How? 1/4🧵

Lovely @xkcdComic on K-means clustering.

Who’s at or coming to the #MNMC conference? The @HTSohLab and I are excited for #biosensors convos and ways to convert #bench2bedside. Also for views in Hawaii!!

support or adaptive technologies, please consider this auction, volunteering, or spreading the word! Org: https://t.co/T5myOxgzMn Auction: https://t.co/qAaxb4ip03 #adaptiveathlete #silentauction #casinoroyale #skiing #accessibilityforall #cycling #rideyourbike #ridebikes (4/4)

only heard through people all her life -- And this only thanks through the countless volunteers and organization of Outdoorsforall that make this possible. Outdoorsforall is having a silent auction, if you want to support providing the outdoors to those that need more (3/4)

make motions she used to not be able to do. Ive seen such a remarkable transformation in her mentally and physically. She cant stop raving about @outdoorsforall! I feel so unbelievably happy to know she's finally able to start seeing and experience so many of the things shes (2/4

This is my mom! Thanks to OutdoorsForAll, my mom has had the best year of her life, and given her new outlooks for what she can do. She's skied, road and mountain biked all for the first time this year! Now, she goes biking for a few miles practically everyday, and can (1/4)

The champagne!! ⁦@NobelPrize⁩ ⁦@CarolynBertozzi⁩ ⁦@Stanford_ChEMH⁩ ⁦@Stanford⁩

California needs more rain for many reasons. This is another reason.

Our paper “Improved immunoassay sensitivity and specificity using single-molecule colocalization” https://t.co/GmqWuXT52I is now featured on the Editors’ Highlights webpage in the “Biotechnology and methods” research section @NatureComms #Biotechnology

Have to really thank co authors @AmaniHariri20 , @steventan17 and our lovely collaboration with @Dunn_Lab. Y’all have helped me learn a lot in the process and I’m so glad to have had the chance to get this idea out together! Thanks for making this fun to tackle together!

Code to determine colocalization and transformations are available here with demo data: https://t.co/IbJwV7GKPk. Full Data available to public:

And when we do that, we lowered our signal variation! Much more work needs to be done to make this sort of method accessible, however it’s a start. Our data and code are free to access and #opensource.

We focus on the problem that bulk measurements of molecules such as those in conventional ELISAs can have very high background signal, esp. in complex media. We decided to zoom in to the single molecule regime, and only count those samples that are forming molecular sandwiches!!

A lot of my phd focuses on solving fundamental limitations in assay design via #modeling, #assay molecular manipulation and #computational tools. This one is a nice synergy between these three.

Check out recent work from my phd with co authors @AmaniHariri20 and @steventan17 out in @NatureComms working towards minimizing background signal that plague immunoassays by using fluorescent colocalization and image processing methods!

Honored to be a part of @BigHatBio this summer as a DS/ML intern! What an awesome company running a supportive and vibrant community working towards big and impactful goals for #therapeutics. 😍