CT-inspired two-photon lets us record glutamate activity from hundreds of synapses at a time, at >1000 Hz. Really happy to share this, and there's more to come!.

RT @AndrewGYork: Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields. This is a fluorescence timelapse of MagLOV….

RT @jdwong_campos: I am thrilled to share my first bio paper ever in @AdamEzraCohen lab with super important contributions from @rhodamine1….

RT @KentaM_Hagihara: We @AllenInstitute for NeurDyn-Ophys are gearing up to launch a platform for in vivo testing of genetic NM/NT/Voltage….

RT @PLeippe: 🎉 Thrilled to share 4 yrs of hard work: iAspSnFR preprint at 🔬 GFP-based aspartate sensor, engineered….

RT @abhi_aggarwal1: iGluSnFR3 is published! Check it out⬇️. We made a new glutamate indicator with improved sensiti….

Our group is looking for a Research Associate interested in helping test new fluorescent indicators in vivo. Fun team. Relocation support to Seattle. Know anyone?

RT @JaimedeJuan: Folks, first paper of our lab is out!!. We discovered that a couple of trans-synaptic proteins, LGI1 and ADAM23, are acute….

RT @Addgene: We wrote a blog post in 2018 about the latest glutamate sensor - but times have progressed and so must we. @abhi_aggarwal1 upd….

RT @fltomaska:

RT @fltomaska: Finding your glute-a-mate has never been easier!.#iGluSnFR3

If you're still reading, check out the paper!!.Special thanks to @ERSchreiter for taking Abhi and me into his lab and teaching us about protein biosensors!.

Rui Liu (Kleinfeld lab) imaged the very distinct feedforward and recurrent input patterns that come into Layer 4 of mouse cortex (400 um deep) using adaptive optics 2P imaging, showing the utility of iGluSnFR3 for input-output circuit cracking.

Yang Chen (Konnerth Lab) performed simultaneous patch and imaging to characterize single-action-potential responses in vivo.

We also found surface display sequences that improve iGluSnFR's localization to postsynapses. Exansion microscopy with help from @TillbergPaul and @boazmohar

@abhi_aggarwal1 screened millions of variants in bacteria. 1000 variants went thru @janeliagenie screen. Abhi found 15 (!) mutations that improve performance in multiple assays. Interestingly, most of them are at the interface between the two domains of the indicator.

@MichaelHoppa , @ARalowicz , and Sam Bergerson investigated this saturation by manipulating synaptic release in culture

This project started with us realizing that the specificity of iGluSnFR for synaptic release (vs spillover) is limited by its saturating activation rate; Figure from Helassa et al 2018. This damps responses close to release sites. Biophysical modeling told us: we can fix that!

Creating and testing new indicators is hard work, and a great team of labs worked on this. @ERSchreiter @janeliagenie @LoogerL @MichaelHoppa + @ARalowicz , Arthur Konnerth + Yang Chen, David Kleinfeld + Rui Liu, and others. Below are some highlights!.

Why did we make a new glutamate indicator? Neural computation hinges on how neurons transform inputs into outputs. We can record spiking output with electrodes/Ca2+ imaging, but recording a neuron's inputs is much harder. Neurotransmitter imaging could solve this!.

Check out our preprint on iGluSnFR3: Inspired by properties of endogenous glutamate receptors, we made an indicator that’s more sensitive and synapse-specific than previous generations. Recording by @fltomaska and @m8ta, @HHMIJanelia