SKI Stem Cell Research Facility
@MSK_StemCell
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Applications on hESCs/hiPSCs. Services highlighted: 1. Reprogramming; 2. All type of genome editing; 3. Tri-lineage differentiation and more.
New York, NY
Joined October 2024
RT @stemcellreports: Online Now: Applying #CRISPR activation to verify single-/dual-reporter hPSC lines, dTAG #knockins & silent gene #knoc….
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RT @efapostolou29: Beautiful work by the Stadtfeld and @TommyVierbuchen labs on the role of genetic variation on imprinting and dna methyl….
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RT @zhangf: We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGR….
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For an inducible approach, we recommend iPEmax if you'd like a cleaner platform that does not interfere with mismatch repair, p53, or RNA metabolism, and you'll still achieve the same efficiency by adding Dox for a little longer, compare to iPE-plus or iPE7.
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For electroporation experiments, we recommend using PE7 directly, as it is currently the most efficient prime editing version. By the way, co-delivery of p53DD (Addgene Plasmid #214080) can further enhance prime editing efficiency when using PE7!.
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Happy long weekend！. This is a quick message:. PE-Plus integrates MLH1dn and p53DD, which together inhibit the mismatch repair and p53 pathways; while PE7 incorporates La, a factor involved in various aspects of RNA metabolism. Both PE-Plus and PE7 are built on PEmax.
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We now offer various inducible PE hPSC lines, including iPE2, iPE4, iPEmax, iPE-plus, and iPE7 (we recently generated and tested it!) for both internal and external researchers, and welcome to collaborate!.
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3. Controllable mutation induction/correction in hPSC-derived cells by adding Doxycycline during the differentiation process or differentiated cell product. 4. Multiplexed genetic variants screening using prime editing approach.
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The plasmids used for the iPE line generation, lenti-ePegRNAs and the backbone have been deposited at Addgene: .
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Potential applications:. 1.Highly efficient precise mutation induction/correction in hPSCs without the DSB and donor template. 2.Convenient to generate multiple mutations in a single hPSC line in ONE step.
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The study was led by our Senior scientist Dr. Youjun Wu and collaborated with Studer lab @TaeWanKim @studerl. We introduced the iPE-Plus platform, enabling efficient and controllable multiplex editing in hPSCs.
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Excited to share our “Robust and inducible genome editing via an all-in-one prime editor in human pluripotent stem cells," is now published @NatureComms .
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RT @DanweiHuangfu: Pleased to share our story @NatureComms on the discovery of genes regulating #hPSC pluripotent identity versus cell fitn….
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RT @MSKCancerCenter: 👏 Congratulations to Dr. Deb Schrag, Chair of the @MSK_DeptOfMed, and Dr. @studerl, Director of the Center for Stem Ce….
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On Oct 7- 11, we will conduct the hands-on training that teaches basic hPSC culture techniques. Topics covered include: hPSC thawing, expansion, passaging, freezing, and solving some technic questions/issues you may have. Reach out zhout@mskcc.org for more info. Welcome to join!.
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RT @biorxiv_cellbio: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells https://t.….
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