Our laboratory website is now available in English! Check out our research!.

Our new paper has been published! .In this study, we revealed the mechanism by which the efficient transcription by RNA polymerase II is accomplished on nucleosomes containing a histone variant H2A.B. #cryoEM.

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Check out our new preprint on H3-H4 octasome transcription by RNAPII!.We discovered that H3-H4 octasome is transcribed more efficiently than nucleosomes and visualized its transcription process by cryo-EM. #cryoEM.

Check out our review article on noncanonical nucleosomes, which differ from canonical nucleosomes in their histone composition and DNA wrapping. The article highlights recent insights into their structural and functional diversity.

Check out our methods paper on high-resolution cryo-EM analyses of nucleosomes is published! It covers nucleosome preparation, grid making, and single-particle analysis for cryo-EM. This work is a collaboration with the Danev Lab at the University of Tokyo.

Check out our paper on the cryo-EM analysis of chromatin derived from human cells! .We have established a technically accessible method for analyzing the chromatin structure prepared from human cells.

Check out the cryo-EM structure of the CENP-A-H4 octasome. This nucleosome-like structure contains a histone octamer composed solely of centromere specific histone variant, CENP-A, and H4!. Amazing work by @NozawaL! Congratulations! 🎉. #Subnucleosome.

Our paper, “The cryo-EM structure of full-length RAD52 protein contains an undecameric ring,” is among the top 10 most-cited papers in FEBS Open Bio (2023)!. Great collaboration with Kagawa-lab (Meisei Univ.)—congrats, Kagawa-group!. 🔗 #CryoEM #RAD52.

Excited to share our paper on the RNAPII-NELF-DSIF-TFIIS-nucleosome complex published @ScienceAdvances , in collaboration with the Sekine lab @RIKEN_BDR ! This study reveals how NELF and nucleosome function in promoter-proximal pausing of RNAPII! 

Check out our paper on the chromatin protein DEK published @NatureSMB! We found DEK as a nucleosome binding protein and its role in promoting H3K27me3 by PRC2! Great collaboration with Gotoh Lab, @YusukeKishi1 , MasumotoG, and @Cell_Tokyo_Tech ! Thank you!

Our new paper about the cryo-EM structures of barrier-to-autointegration factor (BAF) and Lamin A/C complexed with nucleosomes has been published! .This study reveals that BAF and Lamin A/C interact with nucleosomes in multiple binding modes.

Congratulations to Nagamura-san and many thanks to Nureki-sensei for this incredible collaboration! (2/2).

We are excited to share our new paper on the Cas9-nucleosome complex, published in collaboration with the Nureki Lab! This study reveals how Cas9 targets DNA within nucleosomes, advancing our understanding of genome editing. (1/2).

Compare ours with the great works @LucasFarnung @sciJamesWalshe.

Check out our new preprint on transcription-coupled H3K36me3 deposition by Set2 and RNAPII elongation complex (EC)! We visualized EC-bound Set2 capturing reassembled upstream nucleosome intermediate after downstream nucleosome disassembly!.

With collaboration with Sugiyama lab(Kyoto Univ.), Our work regarding Cryo-EM and SAXS analysis of overlapping dinucleosome has been published!.

A new paper has been published! Congratulations to the Uemura lab (Univ of Tokyo) and Takada lab (Kyoto Univ) for our collaborative work on observing nucleosome unwinding using solid-state nanopores!.

RT @osakabe_a: Very excited to share that our new work is finally out🎉.We demonstrate that the chromatin remodeling factor DDM1 “opens” the….

Check out our latest research on chromatin remodeler DDM1 and the H2A.W nucleosome. We were able to visualize how DDM1 opens the the H2A.W nucleosome! This is a great collaboration with Drs Akihisa Osakabe and Tetsuji Kakutani. Congratulations!.

Our new paper about nucleosome containing H3E97K mutation is published! This mutation is found in cancer cells.