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Hiroshi Profile
Hiroshi

@Cell_Tokyo_Tech

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Journey of a Cell Biologist

Joined September 2018
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@Cell_Tokyo_Tech
Hiroshi
2 years
Work on knock-in mice expressing a histone modification probe (mintbody) was published. Mouse lines were generated by Jun Ueda and Kazuo Yamagata (> 10 yrs ago) and the analysis were done by Yuko Sato, Maoko Takenoshita, and Miku Ueoka. Congratulations! https://t.co/bFoxvRQebH
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link.springer.com
Histochemistry and Cell Biology - During development and differentiation, histone modifications dynamically change locally and globally, associated with transcriptional regulation, DNA replication...
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@Cell_Tokyo_Tech
Hiroshi
2 years
A preprint reporting knock-in mice expressing histone H4K20me1-mintbody, by Yuko Sato and former students, in collaboration with Jun Ueda and Kazuo Yamagata. https://t.co/jMIvgepamd
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@Cell_Tokyo_Tech
Hiroshi
2 years
Congratulations Martino and Nadine!
@NVastenhouw
Nadine Vastenhouw
2 years
It’s finally out! The PhD work and first first author paper of @MartinoUgolini: https://t.co/tm5MSGQkOA. Congrats to all co-authors @mkierun, @lanoliin, Hiroshi Kimura, and Haruka Oda for the beautiful work! We also thank @cSabrya @NatureCellBio for the pleasant review process.
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@Cell_Tokyo_Tech
Hiroshi
2 years
Work by Naoki Goto (@Naoki_Goto_), a PhD student, about histone H3K36me2 on heterochromatin was just published, in collaboration with labs of K. Yamagata, H. Kurumizaka, and H. Nishihara. Congratulations! https://t.co/fNItRDfwBA
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@Cell_Tokyo_Tech
Hiroshi
2 years
Work by Masaru Nakao, who is getting PhD, was just published. Mode of SUV420H2 heterochromatin localization through multiple HP1 binding motifs in the heterochromatic targeting module - Nakao - Genes to Cells - Wiley Online Library
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onlinelibrary.wiley.com
SUV420H2 heterochromatic targeting module (HTM) contains three HP1 binding motifs and their cooperative binding to HP1 stabilizes, or locks, HP1 on heterochromatin. An HTM mutant that does not...
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@Cell_Tokyo_Tech
Hiroshi
3 years
A review with Hiroshi Ochiai and colleagues came out. Organization of transcription and 3D genome as revealed by live-cell i...
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@focalplane_jcs
FocalPlane
3 years
Our #MidweekMovie shows actin dynamics in a #zebrafish embryo from Haruka Oda, @Cell_Tokyo_Tech, et al. They find that f-actin accumulates in the nucleus of early embryos & forms patches associating with condensing chromosomes. 📖@BiologyOpen: https://t.co/v42VvAClrZ
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@Cell_Tokyo_Tech
Hiroshi
3 years
Nuclear F-actin dynamics in the early embryo, in collaboration with Nadine Vastenhouw, Shige Kawashima, Motomu Kanai, and colleagues. Congratulations Haruka!
journals.biologists.com
Summary: Filamentous actin accumulates in the nucleus of zebrafish early embryos and forms patches associating with condensing chromosomes during prophase.
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@Cell_Tokyo_Tech
Hiroshi
3 years
Collaborative work led by Yoichi Shinkai revealed what happens to chromatin organization when mouse cells lack both H3K9 and H3K27 methylation marks. Congratulations Kei! https://t.co/IKyZy4cd7W
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academic.oup.com
Abstract. Heterochromatin is a key architectural feature of eukaryotic chromosomes critical for cell type-specific gene expression and genome stability. In
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@Cell_Tokyo_Tech
Hiroshi
3 years
The formatted version of γ-H2AX dynamics paper by Fern (Watanya) is now available. https://t.co/JYSlO288dI
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@Cell_Tokyo_Tech
Hiroshi
3 years
Ph.D. work by Fern (Watanya) is now published. Congrats! Watanya Trakarnphornsombat, Hiroshi Kimura; Live-Cell Tracking of γ-H2AX Kinetics Reveals the Distinct Modes of ATM and DNA-PK in Immediate Response to DNA Damage. J Cell Sci 2023; jcs.260698. doi:
journals.biologists.com
Summary: Live-cell imaging reveals that DNA-dependent protein kinase, but not ATM, plays a primary role in the phosphorylation of histone H2AX during the immediate early response to DNA damages.
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@Cell_Tokyo_Tech
Hiroshi
3 years
Work by Misako Tanaka, a visiting Ph.D. candidate from Tokyo Medical and Dental University, was published. Congratulations! The role of H3K9me3 in oral squamous cell carcinoma
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@Cell_Tokyo_Tech
Hiroshi
3 years
Satoshi Uchino's paper was selected for one of the Year in Cell Biology 2022 articles. Congratulations!
@JCellBiol
Journal of Cell Biology
3 years
Satoshi Uchino et al. develop a new probe for phosphorylated #RNA polymerase II which enables visualization of the sites of #transcription in living cells. https://t.co/zxZiTSlOTP From the Year in Cell Biology 2022: https://t.co/6cR9fhjdBX #CellBio2022
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@Cell_Tokyo_Tech
Hiroshi
3 years
Congratulations Nadine, Ksenia, and colleagues!
@NVastenhouw
Nadine Vastenhouw
3 years
Our story about the spark of life now in published form https://t.co/l0k0eNcE89, so it's time for a shout-out to @currentbiology, @AnneKnowlton1, and our anonymous reviewers for the efficient, pleasant, and constructive review process. Thank you!
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@Cell_Tokyo_Tech
Hiroshi
3 years
Work by Haruka Oda on nuclear filamentous actin in zebrafish early embryos, in collaboration with groups of Nadine Vastenhouw (Lausanne) and Shigehiro Kawashima and Motomu Kanai (Tokyo). https://t.co/85Nr3U1LOB
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biorxiv.org
In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including cell migration, stress fiber formation, and cytokinesis. Recent studies have shown that actin...
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@Cell_Tokyo_Tech
Hiroshi
3 years
Work of a Ph.D. student, Fern (Watanya Trakarnphornsombat). She monitored γ-H2AX accumulation at DNA damage sites in living cells and found different regulations by ATM and DNA-PK.
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biorxiv.org
DNA double-strand break (DSB) is a serious form of DNA damage that can cause genetic mutation. On the induction of DSBs, histone H2AX becomes phosphorylated by kinases, including ataxia telangiecta...
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@Cell_Tokyo_Tech
Hiroshi
3 years
In-person EMBO Laboratory Leadership Course (12/3; Tokyo) and International Symposium for Female Researchers in Chromatin Symposium (12/5; online).
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@Cell_Tokyo_Tech
Hiroshi
3 years
Congratulations Yohei, Takeshi, and colleagues!
@Yo_Hey_Kono
Y Kono
3 years
Excited to share that our latest paper is finally out in @JCellBiol. Much data has been added since the bioRxiv version released in January.
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