We're saying goodbye to and heading over to @bluesky! 🦋 ✈️. Join us there for the next chapter. See you on the other side!🌄. @

RT @ZoltanMolnar64: Special Neuroscience Seminars on SCHIZOPHRENIA – Recommended for FHS “Wiring the brain” and MSC in Neuroscience Brain D….

The Danish Neuro Single Cell (NeuSiC) platform at BRIC opens for requests in mid-February 2025, offering access to advanced single-cell and spatial omics technologies. Funded by the @lundbeckfonden, it supports all steps from sample prep to data analysis. More info: 🧵.

Dive into the transcriptomic landscape of TSC with us. 👀 🧬 . Kudos to @Frederik_friis and all collaborators. 🎉🎇.

7️⃣ Therapeutic Insight: Neuron-specific AMPA receptor signaling, especially auxiliary subunits, could be involved in TSC-related epileptogenesis, presenting a potential target for future therapies.

6️⃣ Metabolic Reorganization: Neuronal—but not glial—cells in TSC tubers exhibit a metabolic shift: reduced mitochondrial respiration and increased fatty acid metabolism, indicating a restructured energy landscape.

5️⃣ mTOR Pathway Dynamics: In TSC tubers, several gene expression changes appear to counterbalance mTOR hyperactivity, highlighting a complex regulation of the pathway in disease context.

4️⃣ Neuronal Changes: Principal neurons and GABAergic neurons in the upper layers of the cortex show the most significant gene expression changes, suggesting a key role in TSC-related changes.

3️⃣ Key Findings: Despite severe disorganization, all neuronal subtypes are preserved in cortical tubers, even rare types. Interestingly, based on smFISH, principal neurons keep their spatial identity.

2️⃣ Methodology: We performed single-nucleus RNA sequencing on cortical tubers from TSC patients and matched pediatric controls to analyze molecular differences at a single-cell level.

🧵. 1️⃣ Background: Tuberous sclerosis complex (TSC) is a genetic disorder caused by mutations in the TSC1 or TSC2 gene, leading to mTOR pathway hyperactivation and epilepsy. Cortical tubers—disorganized brain structures—are a hallmark of TSC, associated with focal seizures.

🚨 Exciting update! 🚨 We've just preprinted our latest paper: "Single-cell profiling of cortical tubers in tuberous sclerosis complex shows molecular structure preservation and massive reorganization of metabolism.". Check the following 🧵:.

RT @biorxiv_neursci: Cortical layer 6b mediates state-dependent changes in brain activity and effects of orexin on waking and sleep https:….

Congratulations to @wol_laura for successfully defending her PhD thesis on 'Differential impact of maternal immune activation on cell types in the developing neocortex'. 🐀🧬🧑‍🔬

Big thanks go to the organizers (right) and the PI (left) who supported such an open and friendly lab environment, prioritising personal growth alongside scientific growth.

Hike in the city and botanical garden to comfort the eyes with lush greenery around. 🌲🌿

The annual 'KoKhos lab' retreat has come to an end after 2 days in Gothenburg, Sweden. 🇸🇪.Lots of hiking, informal scientific discussions and team building sessions. There can't be a better end to the summer than this. ☀️🍹⛰️.Refreshing perspectives on the future themes. 🧘‍♂️

Kokhos lab's annual retreat is about to kickoff. No one except the organizers know where we're headed to!

Prof Konstantin will discuss the application of single cell and spatial omics in translational research during the Emerging Tech Workshop at ARDD2024 on August 26th.🧬. Promising sessions of bridging basic and translational researches leveraging to drug discovery.💊. #ARDD2024