Ken Wolfe Lab
@KenWolfeLab
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Trying to understand yeasts you've never heard of.
Joined July 2016
They found that the thick cell wall of strains with an intact HOC1 gene is the reason that they're difficult to transform with DNA. They have engineered the hoc1 mutation into the OPENPichia platform strain. See Van Herpe et al (2022):.
biorxiv.org
In the standard toolkit for recombinant protein expression, the yeast known in biotechnology as Pichia pastoris (formally: Komagataella phaffii ) takes up the position between E. coli and HEK293 or...
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We found that industrial strains have a null mutation in HOC1 that makes their cell wall thin, so they secrete proteins well. Wild isolates have intact HOC1 and are poor secretors. The OPENPichia group @NicoCallewaert found the same HOC1 mutation by a different route….
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Our study of loci affecting secretion of a heterologous protein in Komagataella phaffii (Pichia pastoris) is published. It’s the first QTL study done in this biotech yeast species….
journals.plos.org
The yeast Komagataella phaffii (Pichia pastoris) is widely used as a host for secretion of heterologous proteins in biotech applications, but all the commonly used expression systems are derived from...
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RT @Kyukimasa: ラガービール酵母の起源.下面発酵するラガー酵母は起源に不明な点があった。.2つの株があって、ひとつは10年ほど前にパタゴニアの木こぶに生息する微生物なのが発見。.今回、もうひとつの株を英国の土壌から発….
irishtimes.com
Scientists at UCD discovered and isolated ancestor of yeast species in a wooded area of Belfield
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RT @yeastpi: Delighted that our discovery of European lager yeast parent is covered by the New York Times
nytimes.com
Scientists found a relative of a parent of lager yeast in soil on an Irish university’s campus, which could help to track how it ended up in Bavaria.
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RT @yeastpi: First European isolates of the lager yeast parent Saccharomyces eubayanus found by University College Dublin undergraduate stu….
academic.oup.com
First isolation of European parents of lager yeasts from a wooded area on a university campus in Dublin, Ireland.
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Our paper on the mysterious flip/flop inversion polymorphism in Saccharomyces species -- It's old but it's young! Congratulations Letal, and thanks @PLOSGenetics for efficient handling.
journals.plos.org
Author summary We show that a region of the genome of Saccharomyces yeast species has toggled repeatedly between two alternative orientations for millions of years. The orientation of this 24-kb...
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RT @yeastpi: Interested in a postdoctoral position studying Candida drug resistance, genomics and evolution, in Ireland? Apply by Jan 7th 2….
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Our preprint on giant GAL clusters in Torulaspora is just out on BioRxiv. Congrats to Anjan on this lockdown project.
Giant GAL gene clusters for the melibiose-galactose pathway in Torulaspora #bioRxiv.
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