Konstantopoulos Lab
@KKLabJHU
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Cancer Metastasis | Cell Motility | Bioengineering @ JHU
Johns Hopkins University
Joined October 2019
Pleased to share our latest collaborative work with the @DevreotesLab in @PNASNews. Using optogenetics, we show that both insufficient and excessive Ras activity impair cell motility, underscoring the need for optimal Ras signaling and cortical tension.
pnas.org
Ras has traditionally been regarded as a positive regulator and therapeutic target due to its role in cell proliferation, but recent findings indic...
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RT @mistrigr: 🎉After years in the making… our work, @KKLabJHU @AveryTTran, Dr Wisniewski & Dr Kalab, is out in Nature Materials!.Anillin &….
nature.com
Nature Materials - Cell migration in confined environments is initiated by a cytoplasmic pool of anillin and Ect2 that promotes RhoA/myosin II-mediated activation at the poles of migrating cells,...
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1. Many thanks to all of @KKLabJHU lab members and our amazing collaborators Drs. Kalab, Stoletov, Lewis, Friedl, Gu, @GerechtLab, Martin and Wu. This wouldn’t have been possible without you!.
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In sum, cytoplasmic Anillin and Ect2 enhance RhoA/myosin II-dependent contractility and bleb-based confined cell migration.
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Confinement enhances Anillin nuclear exit and accumulation at the plasma membrane in a mouse model.
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Cell migration in confining environments of different stiffness and viscoelasticity promotes Anillin accumulation at the cell edges.
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Increasing confinement induces RhoA activation to facilitate cell entry and bleb-based migration.
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Thrilled that our newest work led by @AveryTTran, Dr. Wisniewski, and @mistrigr is out today in @NatureMaterials! We show how the cytokinesis proteins Anillin and Ect2 drive RhoA/ROCK/myosin-mediated invasion in confining spaces in vitro and in vivo.
nature.com
Nature Materials - Cell migration in confined environments is initiated by a cytoplasmic pool of anillin and Ect2 that promotes RhoA/myosin II-mediated activation at the poles of migrating cells,...
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RT @CMBEjournal: I'm pleased to announce that CMBE now has an impact factor of 5.0! And don't forget, there is no publishing fee. https://t….
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Read more 👇.🧩 Nature Materials:accepted in principle on 01/31/2025 and officially accepted today. Stay tuned for its online release!.🧠Science Advances: 🧬 Curr Opinion in Cell Biology: 🔬 Nano Letters:
pubs.acs.org
Cells migrate in vivo through channel-like tracks. While polydimethylsiloxane devices emulate such tracks in vitro, their channel walls are impermeable and have supraphysiological stiffness. Existing...
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Congrats to my brilliant PhD student @alice_amitrano, recipient of the @HopkinsEngineer Doctoral Student Excellence in Leadership Award! A standout leader in our grad community, and scholar with articles in Science Advances , Current Opinion Cell Bio, Nano Letters & Nature Mat.
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RT @LammerdingLab: How do altered levels of lamins and other nuclear envelope proteins contribute to cancer metastasis, and how does this r….
nature.com
npj Biological Physics and Mechanics - Nuclear envelope proteins, mechanotransduction, and their contribution to breast cancer progression
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Immense congrats to my amazing postdoc.@debanikch .for winning the Best Poster Award at the 18th Nano-Bio Symposium at @INBT_JHU ! In just one year, he’s co-led a COCB article , a book chapter , and has several more in the pipeline. Well-deserved!
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RT @ahcells: Three weeks left until the abstract submission deadline for our joint MBI-MPI #mechanobiology conference this September! @MBIs….
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Huge congrats to my first-year PhD student, Josiah Power @realjosiahpower on receiving an @NSF GRFP! At just 19 and with a double-major in ChemBE & Biochem, he took 5 doctoral-level courses last fall (vs. the usual 3) and aced them all. Incredible talent and drive- well deserved!
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RT @JeremyLogue1: Happy to share our latest research article, "The activation of INF2 by Piezo1/Ca2+ is required for mesenchymal-to-amoeboi….
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Excited to share our latest review led by @alice_amitrano & @debanikch! We dive into the mechanisms of confined cell migration, exploring how ion channels, cell cytoskeleton, and fluidic & solid cues drive motility & metabolic reprogramming. Read more:
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💡 hMSCs on soft gels at elevated relative to basal viscosity favor an M2 macrophage phenotype.
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💡 Extracellular fluid viscosity is sufficient to induce adipogenic-to-osteogenic transdifferentiation, not only on purely elastic gels but also on viscoelastic substrates.
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💡Mechanistic cascade: elevated viscosity triggers Arp2/3-mediated actin remodeling, NHE1 activation, and ILK upregulation, which in turn increase membrane tension, thus triggering TRPV4-dependent calcium influx, RhoA/ROCK activity and YAP-dependent RUNX2 nuclear translocation.
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