José Silva & Lab
@Jose_Silva_Lab
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Reprogramming cells for fun and to create embryo models in the tropical Greater Bay Area. @josesilvalab.bsky.social 🇵🇹🇨🇳🇬🇧
Guangzhou/Cantāo 🌴☀️, China中国
Joined June 2018
Very happy to share the work of Huanhuan Li et al. While not a natural embryo, you might not have known that if I hadn’t told you.
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In the latest issue! A complete model of mouse embryogenesis through organogenesis enabled by chemically induced embryo founder cells
cell.com
A small-molecule-only strategy enables reprogramming ESCs into embryo founder cells that give rise to a high-fidelity embryo model directly and efficiently. This embryo model replicates embryogenesis...
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🚨Jobs alert: Want to join our growing team, working on epigenetics in stem cell models of reproduction and pluripotency? We are looking for enthusiastic new lab members as part of the Postdoc, PhD, and MSc student recruitment campaign of our centre:
event.fourwaves.com
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Raising the bar for human post-implantation embryo models
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Signaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling
cell.com
Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells self-organize within 6 days...
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Our October issue is live! The cover highlights several papers reporting advances in stem cell-derived embryo models with a timeline of the rapid development of this field. https://t.co/aGkqvAGN3b
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Honored to see our paper featured on the journal cover! The cover image highlights how the morphological complexity of human stem cell-based embryo models (SCBEMs) has advanced over the past decade.
Our October issue is live! The cover highlights several papers reporting advances in stem cell-derived embryo models with a timeline of the rapid development of this field. https://t.co/aGkqvAGN3b
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Scientists investigated whether STAT3 activation can reprogram pluripotent stem cells into early fates that self-organize into embryo models. 🔎 Discover their findings in @CellStemCell: https://t.co/mThF80hGHd
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Online Now! Signaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling https://t.co/OYi31O6YMc
#stemcells
cell.com
Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells self-organize within 6 days...
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(8/8) Our v1.0, limitations remain (see Limitations section), but we hope, alongside other models, it can help advance human embryogenesis research. Visit our lab website for free access link to our study (under publications section)
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(7/8) There’s more inside: signaling landscapes, PGCs, primary-to-secondary yolk sac transition…
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(6/8) The molecular signatures show strong alignment and high identity scores with human embryo references.
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(4/8) If these cells can generate all early fates, then they should be able to do so in a self-organized manner, forming embryo-like structures……. and they did! (All we had to do was sit back and let the cells do their work.)
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(3/8) The results went beyond expectations: alongside naïve epi, TE & hypoblast, we also saw extraembryonic mesoderm emerge 🤯
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(2/8) From our work in the mouse system we hypothesised that STAT3 could reprogram human cells from a post- to a pre-implantation fate, but the breakthrough came only after we also added TGFβ inhibition.
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(1/8) Very Happy to share our lab’s 3D post-implantation human embryo model, made via signalling reprograming!🙌 Huge thanks to the entire team and our collaborators, special shout-out to Chen Chuanxin (led the work) and Wu Jinyi (computational analysis). https://t.co/iA9sJw9VIY
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(3/3) Fast forward ⏩ @PandaChuanxin @jywu_wu et al. investigated this in human cells. Curious to see what they pulled off? Stay tuned!
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(2/3)… this was actually a side finding in that study🙃 https://t.co/0ULWisHXiU However, we thought this could be paralleling early embryo development and have since been exploring it to generate embryo models.
cell.com
Stuart et al. report distinct routes of reprogramming to naive pluripotency. These differ in their transcriptional trajectories, mechanistic requirements, and developmental parallels, thus demonstr...
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(1/3)A while back, my then-PhD student @H_T_Stuart stumbled on a finding that changed our lab’s direction 👀✨. Turns out, STAT3 mediated reprogramming generates intermediates (Gata6GFP+ cells) going through an earlier developmental stage with increased developmental potential 🚀
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💓 New in @CellCellPress: Researchers at Guangzhou Medical University used #SmallMolecules to convert mouse ESCs into embryo founder cells, which generated a complete embryo model! Article: https://t.co/x9uV3vVZTe Episode with discussion: https://t.co/FL9XdBOMOM
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