After years of telling visitors to Brisbane what an awesome place it is for fun* adventures, I've decided to make a list in the order I remember them of my favourite places to go for ease of reference. Here goes . (*definition of fun may vary).

Reposting comment on preprint of this @Dev_Cell paper. Stats done on technical replicates of L1 KD to make small absolute changes seem significant. Biological replicates were available but not used for stats, why? Because p>0.05? I don't understand this.

RT @PRAndersen: 1/ Our lab’s first preprint found a wonderful journal home! 🎉 . Our paper explores the role of testis-specific transcripti….

Thoughtful and rigorous work from @RippeiH and @PRAndersen teams looking at PAF1C and transcriptional termination. Interesting for me to look at "the other end" of transcription for a change. I wonder what brings tTAF and tPAF together?.

RT @CRGenomica: 🚀 Join the CRG as a Junior Group Leader in Quantitative Cell Biology! 🧬. We’re seeking passionate leaders to tackle fundame….

RT @marcotrizzino: The 5-year postdoc position in my lab is now "live" for applications! Deadline to apply is November 3rd: .

I doubt the stated phenotype here is due to changes in L1 mRNA. The ASO and CRISPRi KD approaches (Figs 1, S1, S2) reduce L1 expression by <10% and these small changes are noted as efficient and significant KD. Stats were done on tech reps to give p<0.05.

RT @the_FrostLab: Looking for a PhD? Interested in epigenetics, imprinting and transposons? Come join the @the_FrostLab and @rjoakey2012 la….

RT @charles_bell92: I am looking for a motivated PhD student who is interested in understanding the fundamentals of gene regulation. They w….

RT @jwjach: interested in 3D genome organization during early development? want to understand how it is shaped by RNA? you like data analys….

Congrats @JuanManuelBotto for passing your final PhD milestone and welcome to the lab @barunlz !

RT @ch_douse: Delighted that this is finally out! Thanks to @Faulkner_Lab @todd_macfarlan and the other reviewer for good and constructive….

RT @maxplanckpress: Calling all emerging scientists - it’s time to send us an application! 😉😎 Lead your own #MaxPlanck Research Group w/ €2….

RT @lh3lh3: Preprint on "BWT construction and search at the terabase scale". We can compress 100 human genomes to 11GB in 21 hours, find SM….

RT @JakobssonLab: Proud to have contributed to this fantastic study led by @ch_douse . HUSH for the world! .

RT @AEpiA: 1/11 Welcome to our August ECR spotlight! This month we get to know Charles Bell @charles_bell92 who works in the Faulkner lab @….

RT @Al__Forrest: Just under a week to apply to join our team working on the spatial transcriptomics (#MERSCOPE, #VisiumHD, #Xenium, and mor….

RT @aachinger: Please RT! 🧬🧬.I am looking for a #postdoc fellow to lead a new project in 3D chromatin organisation and transcriptional regu….

In sum, my alternative hypothesis was not proven and the RNLTR12-int siRNA doesn't seem to impact Mbp. We discussed all of this with the authors as we went along but I also thought there was a good chance someone else might see the same thing - if so there's an answer here. 5/5.

Juan made a HEK293T line (where RNLTR12-int should be absent) stably expressing rat Mbp and introduced the RNLTR12-int siRNA, then used the published Mbp qPCR primers to evaluate the siRNA's effect on Mbp compared to a non-targeting siRNA. No significant effect was observed. 4/n

Thinking about the mechanistic model, I wondered if instead the RNLTR12-int siRNA directly targeted Mbp. Aligning the two sequences I noticed the siRNA seed region matched Mbp pretty well. @JuanManuelBotto volunteered to test this possible off-target match experimentally. 3/n