David Cowburn
@DavidCowburn1
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Joined February 2021
Outstanding design/analysis for molecular condensates.
Biomolecular condensates form spatially inhomogeneous network fluids:
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RT @CEITEC_Brno: One of the most prominent and internationally recognized experts in the field of structural biology, Professor Vladimír Sk….
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Congratulations to @DShechter lab and collaborators on Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency
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Congratulations to @B_Lorton23 , Chris Warren and @DavidShechter for their new work on glutamylation as a PTM .A pleasure to be a contributor.
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Presumably 1.Marley J, Lu M, Bracken C. A method for efficient isotopic labeling of recombinant proteins. J Biomol NMR. 2001;20(1):71-5. Epub 2001/06/30. doi: 10.1023/a:1011254402785. PubMed PMID: 11430757.
@chris_waudby I read a paper where they grew their cells in unlabelled media (LB) to OD of 0.6-1, spun it down, and resuspended it in minimal media. They let the cells recover for an hour before inducing with IPTG, and apparently the yield increased. I tried with 2xYT and M9 and it works!.
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New experimental advances on the nuclear pore complex . and a review .
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New article from the Muir and our labs "A Conserved Histidine Residue Drives Extein Dependence in an Enhanced Atypically Split Intein" 10.1021/jacs.2c08985 . Congratulations to all authors!
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While alphafold and similar have done a great job on single domain well folded systems, as predictions as discussed here. But the level of prediction for complexes and for systems with significant intrinsic disorder is still limited, and requires extensive experimental work.
"Determined" and "solved" are words reserved for experiments, not to predictions. They predicted structures. But predictions shouldn't be taken as facts. Too many fields of science suffered and still do by wrong predictions and computer simulations
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Important step forward in in situ investigation of IDPs.
This is a story about Game of Pores — how to capture a disordered dragon in a black hole. 👇🐉⚫️
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Important assessment of quality of predicted structures.
Online now! The accuracy of protein structures in solution determined by AlphaFold and NMR
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RT @EinsteinMed: The new Einstein Institute for Advanced Studies in the Life Sciences seeks to provide answers to major scientific #lifesci….
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Excellent broad view of new applications in chemical biology.A Chemical Biology Primer for NMR Spectroscopists.Evan T. Clark1,2,#, Elanor E. Sievers1,#, Galia T. Debelouchina1,*.
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Excellent review. .
Our review of the past ~20 years of in-cell solution NMR has just been published OA in @ACSChemRev. These years have been exciting, I look forward to what lies ahead!.@cerm_cirmmp @UNI_FIRENZE @Unibo .
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RT @rpierattelli: 🥳Check out our new Chemical Reviews article about 13C direct detected NMR for biomolecular applications! ..
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A position for a PostDoc is available immediately in my lab at Albert Einstein College of Medicine, on NMR based structural biology. Areas are intrinsically disordered systems in the nuclear pore and protein engineering using inteins. Details -
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A position for a PostDoc is available immediately in my lab at Albert Einstein College of Medicine, on NMR based structural biology. Areas are intrinsically disordered systems in the nuclear pore and protein engineering using inteins. Details -
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