Castaneda Lab
@Castaneda_lab
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We use molecular biophysics, cell biology, and structural biology to study protein quality control mechanisms in ALS and other disorders @SyracuseU.
Syracuse, NY
Joined February 2018
Phase separation of polyubiquitinated proteins in UBQLN2 condensates controls substrate fate:.New publication with Dan Kraut's lab at Villanova where we look at proteasome and deubiquitinase recruitment and activity in UBQLN2 condensates!.
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Welcome aboard @shaharsu ! So excited to have you here and forge new scientific directions!.
Say hello to the new professors joining A&S this fall 👋 . ➡️
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Super grateful to our lab and our fantastic collaborator Dan Kraut and his wonderful team!.
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And promiscuous deubiquitinases (DUBs) can exhibit linkage-dependent activities in the presence of UBQLN2-polyUb condensates (e.g., K63-Ub4 is protected from DUB activity - black curves below)
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Importantly, UBQLN2 condensates with K63-polyubiquitinated substrates would protect these substrates from proteasomal degradation even when proteasomes are recruited into condensates. This behavior depended on the efficiency of UBQLN2 oligomerization (wild-type vs. dSTI1-II)
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Consistent with prior results (, we found that K63-polyubiquitinated substrates sedimented and phase separated with UBQLN2 more efficiently than K48; but both polyubiquitinated substrates can drive UBQLN2 phase separation at very low uM concentrations.
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RT @EliasAdriaensse: Super happy to see the final version of our paper on the role of NAP1/SINTBAD in PINK1/Parkin mitophagy!. @NatureSMB….
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Super excited to have you as one of our Biology colleagues @SUArtSciences ! Go #IDPs! Looking forward to all the cool ideas that come out of your lab integrating IDP biophysics and plant biology! Welcome aboard!.
I am thrilled to announce that I will be joining the Biology Department at Syracuse University as an Assistant Professor in 2025!
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Super excited to have you as one of our colleagues @SUChemistry @SUArtSciences ! Welcome aboard! I second everything you've written in your thread. We will do our best to help drive your awesome science and collaborations! #IDPs.
I'm excited to announce that our lab is moving to @SyracuseU!! We will continue our exploration of how (disordered) proteins sense and respond to changes in their environment, and 📢HIRING AT ALL LEVELS. Get in touch if you're interested! Some details on this move below: 1/5
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RT @LovelessRadio: We had a blast helping out @PattesonLab with #localizationmicroscopy and #zeissAiryscan in @Castaneda_lab and @SU_BioIm….
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RT @DellTech: Recipes. Recipes that are more than their ingredients. Recipes written by hand and lost to time. Can now be analyzed and….
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RT @LabStrader: My lab jokes that IAA does not stand for 'indole-3-acetic acid', but rather for 'involved in almost anything'. Here is a re….
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Grateful to @SUBiology colleagues like @LovelessRadio for getting us into microscopy and cell work many years ago!.
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#cellbio2023 ASCB attendees - interested in #UBQLN2 #condensates and their roles in cell stress response? Visit Anitha's poster on Sun 12/3 (P1589) to hear about heat stress in cells, while Billy presents on UBQLN2 puncta formation with the @oniHQ nanoimager (P1594)!.
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Recruiting #PhD Bio students to @SUBiology ! If you're at @ABRCMS , come talk to current grad Savannah from @sarah_hall02 lab, and undergrad Alana from @YazBraimah lab! @drhougs has all the info - learn about @SUBioInspired , Neuroscience & our growing dept! (apply by 1/4/24)
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RT @alexholehouse: Interested in understanding how and why IDRs matter for cellular function? . @BBKrage and I took a deep dive into this q….
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RT @VKohler_Lab: Please RT!. 🔬Join the #KohlerProteostasisLab!.We have a fantastic opportunity for a #PhD position in #MedicalScience!. 🎯Ou….
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RT @Nicolas_Fawzi: NMR of glutamine-rich prion-like domains in a condensed phase - new stuff from Sandy Wang and @TongyinZ . Structure and….
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Looking forward to hosting a signal transduction and condensates interest subgroup at @ASBMB annual meeting #discoverbmb in March 2024 with @andersenlab ! More info on this soon.
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Thanks @preLights and @_AniruddhaD for highlighting this work!.
Studying the underlying molecular grammar of phase-separating systems. The @Castaneda_lab shows that the N-terminal region of Ubiquilins is indispensable for distinct phase separation behaviour. #preprint. preLight prepared by @_AniruddhaD (@TheMALab)!.
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