Shahar Sukenik
@shaharsu
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alternating between running my lab and taking care of the kids. Tweets my own and mostly science-related.
Syracuse, NY
Joined March 2009
📢New paper alert!! With @boothbylab @alexholehouse @fried_lab we take a look at how an entire proteome survives desiccation. TLDR: Desiccation tolerance is encoded in specific residues on the protein surface, and occurs primarily in biosynthetic enzymes.
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RT @eflores500: 📢 Excited to share our research with @shaharsu & @mvstaller on how activation domain (AD) structure affects gene expression….
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New work from @eflores500 examines the relationship between disordered ensemble structure and activity in transcription. Read more here 👇.
📢 Excited to share our research with @shaharsu & @mvstaller on how activation domain (AD) structure affects gene expression! 🧬 TLDR: HIF-1α & CITED2 ADs suggest ensemble dimensions regulate transcription in unique ways. 🧵 Highlights:
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RT @ProteinMagnus: It has been more than four years since @alexholehouse and I started @IDPseminars and now it is time for fresh blood and….
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RT @haleymoran23: Check out this fascinating blend of cutting-edge structural proteomics and proteome-scale adaptation to the extreme. I am….
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This study is funded by @NSF , with help from @WALIIAnhydro and the NSF's IntBIO program. Molecular graphics and animations by.@janetiwasa. Read the full paper here:
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We propose that proteins have an adaptable, chemically-encoded ability to survive desiccation, and that this feature is distributed in the proteome such that upon rehydration the cell’s proteome is remodeled to favor basic survival over growth.
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We also find that tolerant proteins play very specific roles in the cell, namely the production of small molecule building blocks. Conversely, intolerant proteins are heavy energy consumers - namely ribosomes and ribosome-biogenesis proteins.
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We find that tolerant proteins - those that resolubilize and retain their structure following rehydration - are loaded with negative (but NOT positive) charges and small amino acids (Ala/Gly/Pro) at their surface.
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We subjected yeast lysates to a round of desiccation and rehydration. We use a pelleting assay to assess which proteins aggregate irreversibly, and limited proteolysis to assess which proteins can retain their structure following rehydration.
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Desiccation occurs in an increasing number of ecosystems. Some cells routinely survive it; for others, like mammalian cells, it is lethal. To survive, at least some of the cell’s proteome must be active. We look at the proteome of budding yeast to understand how this works.
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RT @GrcIdp: First, Elizabeth Rhoades of @LaserSharkLab who will be a faculty mentor and serve on the IDP GRS Navigating the Funding Landsca….
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RT @GrcIdp: Our next IDP GRS faculty mentor and "Navigating the Funding Landscape" discussion panelist is @DanfengCai .
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RT @VictorMunoz_UCM: For anybody still interested in learning what makes proteins fold and unfold slow or fast, read our recently published….
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