Fantastic collaboration with @PhilGuo1 and his team to determine the in situ structure of Tad pili. Great work from Greg Whitfield for our part of the work!

Please repost: Postdoc position in antibiotic discovery in a multidisciplinary project w. high-throughput approaches, microscopy, AI, and hit-to-lead optimization @UMontreal @med_umontreal @Mila_Quebec @IRIC_umontreal. Apply @ ML.Antibiotics@gmail.com. See https://t.co/2DjrErxpqw

We're currently looking for a PostDoc position at @UMontreal and @Mila_Quebec to work on a multi-modal bacteria representation for anti-biotic discovery, including multiple modalities: - Phenomics - Transcriptiomics - Structural bio - Viability curves - ADME The work will be

🚨 Call for a POSTDOC in machine learning for drug discovery at @UMontreal 💊 The position will be part of a large collaboration with @BrunLabCaulo Audrey Durand @dom_beaini Anne Marinier @IRIC_umontreal @Mila_Quebec. All details here: https://t.co/ST7Zu85NSI Please RT 💜

Please repost: Postdoc position in AI for drug discovery in a large collaboration with Dominique Beaini, Audrey Durand, Alex Hernández-García, Anne Marinier @UMontreal @med_umontreal @IRIC_umontreal @Mila_Quebec.

Starting to work on my talk at Cambridge tomorrow. Different topic. No Guinness yet.

On my way to @WarwickLifeSci to give a seminar tomorrow on a relatively new project. Thanks for the invitation @davidroper2

Excited to present our resent progress on our antibiotic discovery pipeline at the @bioMerieuxCA AMR Symposium in Toronto Nov. 20th at 11:30 AM.

2 days left. Asst/Assoc Prof position in bacteriology, Université de Montréal. Deadline ***Nov. 15*** come join our growing community of bacteriologists. BTW, à propos of nothing, Canada is the USA’s Bluesky!

Expanding our analysis, we find a distribution of elongation modes, suggesting their evolution is shaped by multiple, independent events, implying a high degree of phenotypic plasticity in the regulation of localized elongation modes. How does your favorite species elongate?

Not only do these three closely related species have different modes of cell elongation but they also have different morphologies. Could the elongation mechanisms in these species influence their morphology or vice versa?

Strikingly, we find that another closely related species, Asticcacaulis biprosthecum elongates from the new pole in addition to elongating unidirectionally from the midcell like A. excentricus.

Comparing FDAA dual pulse labeling without and with mecillinam shows that PBP2 is also required for the directionality (towards the new pole) of PG synthesis.

When we inhibit A. excentricus PBP2 with mecillinam, cells lose elongation control and bulge on the new pole side of the midcell, indicating that PBP2 is indeed required for unidirectional midcell elongation.

In A. excentricus, the PG transpeptidase PBP2 localizes at midcell during midcell elongation, contrasting with its dispersed pattern in C. crescentus. This spatial organization appears crucial for directing elongation patterns.

The dual pulse result is quite different for A. excentricus, with the first FDAA on the new pole side of the second, indicating unidirectional midcell elongation towards the new pole.

Using successive short pulses of a red then a green FDAA, the pattern reveals the known bidirectional midcell elongation of Caulobacter.

Using FDAAs, we labeled the PG of closely related Caulobacter and Asticcacaulis excentricus and let them grow. Dark areas indicate where new PG insertion occurs. As shown previously, Caulo elongates from midcell in both directions but excentricus only towards the new pole!

How does your favorite species elongate?🧵"Phenotypic plasticity in bacterial elongation among closely related species" by @mariedelaby, @liuyang1125 et al https://t.co/7hZOs8O7Bd. How do cells elongate, all over, poles, middle? Close species do it the same way right? Not quite!

Now out in NSMB for those who missed our biorxiv back in 2023..