Scott Gardner
@scott_d_gardner
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Structural biologist with a passion for all things cryoEM. Senior Applications Scientist @ThermoFisher.
London, England
Joined August 2016
RT @BubeckLab: After a hard year, I’m glad to see this finally over the line. A real team effort from @YiboJin @scott_d_gardner @TomasV….
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RT @BubeckLab: Come join our structural biology facilities team at.@ImperialLifeSci on the South Kensington Campus. If you're from #TeamXRC….
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Lastly thanks to @luminous_lab and @bradyajohnston for their amazing YouTube tutorials for protein visulisation in Blender! 5/5.
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Highlights include a neat novel asymmetric GroEL structure (see the pretty render) that explains how the chaperonin can simultaneously retain bound substrate and recruit the co-chaperonin GroES 4/5.
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We used a Chameleon (Thanks to @SPTLabtech and @MDarrow7!) to ameliorate these effects by reducing the time between sample application and plunge-freezing 3/5.
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In our initial #cryoEM experiments, GroEL complexed with nucleotide and non-native substrate suffered from partial denaturation and preferred orientation 😭 2/5.
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Pleased to share a new preprint resulting from my PhD work using #cryoEM on #GroEL, completed in Helen Saibil's lab at @BirkbeckScience😄#cryoEM 1/5
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RT @quantifoil: Great start to @ccp_em Spring Symposium #bcium2023 with @SPTLabtech chameleon workshop on #cryoem sample prep. Fantastic ta….
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RT @BubeckLab: Our article was selected by @NatureComms Editors’ Highlights showcasing the 50 best papers recently published Structural bi….
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RT @BubeckLab: Funded by @ERC_Research, a new study from the lab out in @NatureComms discovers how a tiny protein called CD59 provides a l….
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RT @BubeckLab: Interested in #cryoEM and #biophysics? We’re recruiting with @markianwallace to investigate how bacteria evade immune killin….
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RT @BubeckLab: Interested in integrating #cryoem and membrane #biophysics to understand how the immune system works? We’re recruiting! http….
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RT @BubeckLab: Excited to share new work out in @NatureComms showing how human cells are protect from overactive immune responses. Combin….
nature.com
Nature Communications - To prevent unregulated complement activation, extracellular chaperones capture soluble precursors to the membrane attack complex (sMAC). Here, structural analysis of sMAC...
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This is great! So many possibilities.
Time-resolved cryo-EM using Spotiton
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Very relieved to know that after 6 months, I can still insert a side entry cryo holder without a vacuum crash 😬 #itsthelittlethings
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RT @BubeckLab: Couldn’t have done it without the excellent resources and “beamline” scientists @DiamondLightSou eBIC. .
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Thank you so much and congrats @BubeckLab! So excited to see this published! 😁.
@anaismenny Definitely a huge team effort from @bubecklab members past and present (@CourtneyBoyd1 @scott_d_gardner @anaismenny and @sernarina) spanning 2 years, 8 data collection sessions, and 1 baby.
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RT @BubeckLab: Our latest #CryoEM structure just out @NatureComms shows how proteins pierce lipid bilayers to fight infection. Great coll….
nature.com
Nature Communications - The complement membrane attack complex (MAC) is a lytic immune pore that kills pathogens. Here the authors use cryoEM to provide a structural and biophysical mechanism for...
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