Josh Selfe
@joshsselfe
Followers
44
Following
62
Media
6
Statuses
30
Neuroscience PhD student in the Akerman lab @OxNeuro. Intrigued by cortical circuits, all-optical techniques, sensory processing.
Oxford, United Kingdom
Joined June 2011
RT @SumedhaNalluru: Our paper "Memory reactivation generates new, adaptive behaviours that reach beyond direct experience" is now out https….
0
8
0
Huge thanks to @Abdelfattah_Lab, @ERSchreiter, and @rhodamine110 at @HHMIJanelia, and to @RichBur, Sarah Newey, and Colin Akerman at @PharmOxford for all of the help and support along the way! And to @MPrag for the photo of the beautiful orchid 📷 (7/7).
0
1
3
snRNAseq by Teresa Steyn, computational modelling by @EranShorer and @KiraDusterwald, and IHC by @AKraitzick. All with @UCT_HUB, @UCT_NI, @UCTIDM, and @UCTHealthSci. (6/7).
1
0
2
ORCHID is additionally advantageous in small cells or subcellular compartments, and opens the doors for using all-optical methods to assess driving force more generally. (5/7).
1
0
1
We use ORCHID to confirm the mechanisms that establish Cl- driving force, reveal novel differences between neurons and astrocytes under different network states (1), and make the first in vivo measurements of intact Cl- driving force in mouse cortical neurons (2). (4/7)
1
2
4
ORCHID is an order of magnitude faster than traditional electrode-based techniques, and is unique in leaving intracellular ions undisturbed. (3/7).
1
0
1
In the brain, inhibition is mediated by chloride-permeable receptors. ORCHID combines voltage imaging and a chloride-permeable, light-activated channel to provide a high-throughput, easy-to-use tool for measuring the driving force across inhibitory receptors. (2/7)
1
0
1
Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms
1
14
33
ORCHID is additionally advantageous in small cells or subcellular compartments, and opens the doors for using all-optical methods to assess driving force more generally. (5/7).
0
0
0
We use ORCHID to confirm the mechanisms that establish Cl- driving force, reveal novel differences between neurons and astrocytes under different network states (1), and make the first in vivo measurements of intact Cl- driving force in mouse cortical neurons (2). (4/7)
1
0
0
ORCHID is an order of magnitude faster than traditional electrode-based techniques, and is unique in leaving intracellular ions undisturbed. (3/7).
1
0
0
In the brain, inhibition is mediated by chloride-permeable receptors. ORCHID combines voltage imaging and a chloride-permeable light-activated channel to provide a high-throughput, easy-to-use way of measuring the driving force across inhibitory receptors. (2/7)
1
0
0
Amazing work by @gsgothard showing the developmental origins of the local inhibitory subnetworks of L2/3 pyramidal neurons. Give it a read!.
🚨new preprint alert!🚨 very excited to share the story of my PhD work :) including: development of excitatory-inhibitory circuits, lineage labelling, in vivo patching and re-thinking blanket inhibition. See below for a quick summary! [1/17].
0
0
1
Super excited to share that this preprint is now out, the result of my MSc, and the first all-optical reporter of ionic driving force!.
We are excited to present ORCHID, a new tool for all-Optical Reporting of Chloride Ion Driving force. In the brain, inhibition is mediated by Cl- permeable receptors. ORCHID can therefore be used to quantify the driving force across inhibitory receptors.
0
1
12
RT @ewadorotaz: #zebrafish larvae imaged on home-built #lightsheet microscope assembled by a team of remarkable students Shawniya, Josh @jo….
0
5
0
RT @RichBur: Thrilled to announce the release of our new preprint. Here we show how fast synaptic inhibition adapts to the level of cortica….
0
21
0
I #choosethebluefish because our oceans are beautiful and so full of life and provide life to many. Thank you @MSCinAfrica for giving us a way to support sustainable seafood by looking for the blue fish! With your help future generations will have pristine oceans @WavescapeSA.
0
2
2