This shows the power of cell-type-specific RNA profiling to uncover hidden vulnerabilities. EPAC2 inhibition could be a game-changer for FXS. So excited for what’s next! Full preprint ➡️ #Neuroscience #FragileX

And it worked. ✨ Restored cortical circuit function ✨ Ameliorated sensory behavioral phenotypes Targeting EPAC2 rescued key Fragile X features — offering a new therapeutic path forward! 🔥

EPAC2, a cAMP-activated synaptic regulator, became our focus. We tested whether blocking EPAC2 could fix the circuit deficits. Using ESI-05, a selective EPAC2 inhibitor, we treated Fmr1 KO mice.

Of hundreds of differentially expressed genes (DEGs), only 184 were shared across both neuron types. Amazingly, just one gene — Rapgef4 (Epac2) — was a brain-enriched ASD risk gene and a direct FMRP target. 🧬

Using cell type-specific RNA-seq in Fmr1 knockout (KO) mice, we found opposite effects: genes were upregulated in excitatory neurons but downregulated in PV inhibitory neurons. Dramatic shifts in brain wiring.

https://t.co/QGvdq8T1Qv Excited to share my postdoctoral work! We mapped how loss of FMRP alters excitatory and inhibitory neurons in Fragile X Syndrome (FXS), the leading monogenic cause of autism. Huge molecular divergence — and a new therapeutic target! 🧵

https://t.co/QGvdq8Su0X

Paper is out! @NatureComms Mapping dense neural projections at the single-cell level is challenging—but we tackled it with axonal BARseq, a new technique that maps long-range projections from >8000 neurons in a single brain. 🧠 @TonyZador https://t.co/Mty01CnBsg

🚨Thrilled to see my main postdoc work now published @NeuroCellPress! 🪄 https://t.co/esEUYd5y4J 🧵1/6👇

Microsoft Research has released BioGPT, a large language model trained on biomedical research literature. The model achieves better-than-human performance on answering questions from the biomedical literature, as evaluated on PubMedQA. The code for the https://t.co/MEPL0wmjFo

A chemical regularly used to image cells can dramatically alter the way cellular compartments called condensates look under the microscope. https://t.co/MblaT6Hvfi

PREPRINT🚨Ever wonder how electrical⚡️ stimulation of the brain🧠modulates neuronal activity? Are you working with brain stim and variability makes you want to smash your pc and go to a mountain to grow tomatoes? Are you a family member or a friend who really appreciate me? 1/13

Massive Multiplexing of Spatially Resolved Single Neuron Projections with Axonal BARseq

✨C. Portera-Cailliau and myself were really pleased and honored to write this Preview for @NeuroCellPress about a recent tour de force from Natalia de Marco Garcia’s lab 👉🏼

New preprint ! Developmental bio/neurobiology crisis ?! Still, I am pleased to share my postdoctoral work deciphering neonatal interneuron alterations in a mouse model of Fragile X syndrome - Portera-Cailliau lab, UCLA. Thread 1/10 https://t.co/GfSitalJF7

BARseq can now reconstruct axonal projections at high-throughput! Below: Projections of 100 neurons from a single brain, subsampled (for display purposes) from the 8607 neurons recovered from barcoded viral library injection into auditory cortex. Great work by Li Yuan

Very proud of what BioRender has become thanks to our amazing team. We built BioRender b/c I saw firsthand how much scientists struggled to draw in PPT. It's bio-focused but we're building out chem, physics & more🙂 Also the base version is free, forever💙  https://t.co/MDWa1BVzh7