Zongliang (Carl) Jiang
@ZongliangJiang
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Reproductive biologist @ UF
Gainesville, FL
Joined March 2022
Excited to share our new study on embryo m6A👇.A great collaboration with @ChenghangZong @JainLaboratory and Dr. Chuan He lab. Single-nucleotide Resolution Epitranscriptomic Profiling Uncovers Dynamic m6A Regulation in Bovine Preimplantation Development
biorxiv.org
RNA N 6-methyladenosine (m6A) plays a crucial role in regulating gene expression during early embryonic development. However, the m6A dynamics at single-nucleotide resolution in preimplantation...
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Particularly, inducible KLF2/NANOG enhances naive pluripotency and chimera formation in bovine.
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Thrilled to share our new study for the development a universal 6iL condition for ESC derivation across five mammalian species including mice, rat, bovine, rabbit, and humans. A great collaboration with Qilong Ying lab at USC and Jie Xu at U. Michigan.
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A Universal 6iL/E4 Culture System for Deriving and Maintaining Embryonic Stem Cells Across Mammalian Species
biorxiv.org
The derivation of authentic embryonic stem cells (ESCs) from diverse mammalian species offers valuable opportunities for advancing regenerative medicine, studying developmental biology, and enabling...
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Thrilled to share our paper @CellReports where we established bovine XENs and a new blastiod protocol- Another milestone in bovine stem cells! Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation: Cell Reports
cell.com
Ming et al. report a robust and efficient protocol for the derivation of bovine XENs from blastocysts. Importantly, this study also demonstrates the utility of bovine XENs in elucidating the mechan...
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RT @antonioregalado: Move over, Dolly the Sheep. Synthetic embryos could become "cloning 2.0". You might be able to generate thousands and….
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Here we describe a molecule (METTL7A) acts in the pre-implantation period to attenuate oxidative stress that enhances embryo development to the blastocyst stage and subsequent conceptus elongation in cattle.
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METTL7A improves bovine IVF embryo competence by attenuating oxidative stress
biorxiv.org
In vitro fertilization (IVF) is a widely used assisted reproductive technology to achieve a successful pregnancy. However, the acquisition of oxidative stress in embryo in vitro culture impairs its...
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The bXENs and blastoids established here represent valuable cell models to study embryogenesis and the pregnancy loss. Upon further test their developmental potential, bovine blastoids will help develop new ARTs for improving reproductive efficiency in livestock species (6/n).
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Finally, we developed an improved bovine blastoid using bXENs. We showed that this 3L-blastoids have a similar proportion of hypoblasts and a slightly higher ratio of epiblasts compared to day8 blastocysts, with majority hypoblasts surrounding epiblasts (5/n).
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Furthermore, by treating bovine IVF embryos with bXENs signaling cocktail, we showed that the growth and progression of epiblasts are also facilitated in the developing pre-implantation embryo (4/n).
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We implemented a 3D co-culture model with bXENs to examine whether or how hypoblast regulates the development of epiblast during bovine embryogenesis. We demonstrated that the presence of bXENs and associated communications support the growth and stemness of bEPSCs (3/n).
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We conducted comprehensive molecular characterization of bXENs. Transcriptomic annd epigenomic analyses confirmed the identity of bXENs and revealed that they are resemble hypoblast lineages of early bovine peri-implantation embryos (2/n).
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We found that a chemical cocktail (FGF4, BMP4, IL-6, XAV939, and A83-01) enables de novo derivation and long-term culture of bXENs (1/n).
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We are so excited to share our work on the establishment of bovine extraembryonic endoderm cells and another bovine blastoid model for studying embryogenesis. Congratulations to all co-authors.
biorxiv.org
Understanding the mechanisms of hypoblast development and its role in the implantation is critical for improving farm animal reproduction, but it is hampered by the lack of research models. Here we...
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A great collaboration with @AndrejSusor lab!.
Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development
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Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development
biorxiv.org
Translational regulation is pivotal during preimplantation development. However, how mRNAs are selected for temporal regulation and their dynamic utilization and fate during this period are still...
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RT @repro_radio: Livestock make great models to understand the fundamentals of reproduction! Wonderful talk by @ZongliangJiang from @uf_ans….
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Thank you @IETS_org.
Dr. Zongliang (Carl) Jiang, recipient of the IETS Foundation 2023 Early Career Achievement Award, Scientist, for his contribution to understanding epigenetic mechanisms regulating pre- and peri-implantation development when most pregnancy losses occur.
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