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Valdivia Lab

@ValdiviaLab

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Durham, North Carolina
Joined August 2019
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@ValdiviaLab
Valdivia Lab
2 years
RT @MicroscopyLAC: The @DukeU Light Microscopy Core Facility (LMCF) is hiring a microscopy specialist to assist w users & trainings. Experi….
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@ValdiviaLab
Valdivia Lab
2 years
RT @emboreports: Read Heran Darwins and co-authors new piece on confusion!. @HeranDarwin @AldridgeLab @jcslab @ShenLabTufts. -> https://t.c….
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@ValdiviaLab
Valdivia Lab
2 years
RT @BrodskyIgor: The Brodsky lab is looking for a senior research investigator! If you have 5+ yrs post-PhD research experience, are intere….
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@ValdiviaLab
Valdivia Lab
3 years
RT @valdi001: Universities can help by allowing us to pay postdocs more competitive salaries.
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@ValdiviaLab
Valdivia Lab
3 years
We bid farewell to @eldoles and @durstingly, two outstanding scientists and colleagues. The positive impact of their contributions, both as mentors to junior lab members and in their research projects, will be felt for years. Best of luck with the next adventure!
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@ValdiviaLab
Valdivia Lab
3 years
RT @IsabelleDerre: My lab is hiring at all levels. We are looking for talented and motivated people to work on Chlamydia-host interaction r….
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@ValdiviaLab
Valdivia Lab
3 years
Wonderful program!.
@DukeBioCoRE
Duke BioCoRE Program
3 years
Please share broadly:.The BioCoRE application for the 2023-2024 school year is now live!. Prospective students can apply here: If you'd like to learn more about our program before applying, you can visit our webpage:
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@ValdiviaLab
Valdivia Lab
3 years
Bittersweet day as we wish @lauren282012 a safe trip to 🇨🇦 to start her own lab at @uvic Her work on #Akkermansia genetics has been foundational. In her honor we had 🥞 and celebrated all things Canadian
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@ValdiviaLab
Valdivia Lab
3 years
Useful guidelines!.The Dos and Don'ts of Text Recycling |
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@ValdiviaLab
Valdivia Lab
3 years
RT @profpathogen1: My #microbiology students prepare an infographic on a microbe for their final project. They can choose virus, eukaryote,….
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@ValdiviaLab
Valdivia Lab
3 years
So what does this mean? It is possible that metabolites produced during mucin consumption dampen the expression of lipid metabolism genes. Whether this contributes to the positive effects of Akkermansia on metabolic health remains TBD. Lots to do but stoked that we now have tools.
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@ValdiviaLab
Valdivia Lab
3 years
Finally, we asked how does the GI respond to mucin consumption by Akkermansia? We used GF mice mono-colonized with wt or Mul mutants and did RNAseq from colons. One of the strongest differences was the repression of mevalonate/cholesterol biosynthetic genes in WT Akkermansia.
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@ValdiviaLab
Valdivia Lab
3 years
MUL mutants were perfectly capable of colonizing GF animals. But they failed miserably if there were other microbes around. Therefore, the ability to use mucins gives Akkermansia a competitive edge.
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@ValdiviaLab
Valdivia Lab
3 years
We identified two loci encoding genes that may be distantly related to PUL systems of Bacteroides- we named these MULs for mucin utilization loci. One of the predicted proteins in one of these loci is Amuc1100, a factor previously associated with immunomodulation.
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@ValdiviaLab
Valdivia Lab
3 years
IN/TnSeq in mice confirmed that the same genes needed for growth in mucin were also needed to colonize GF and CONV mice. and Muc2KO mice (lack major GI mucin). So food sources in the GI are more diverse.
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@ValdiviaLab
Valdivia Lab
3 years
We next applied IN/TnSeq to probe the genetic requirements for mucin use. Bottom line: Akkermansia needs to synthesize many of their own AA to grow only in mucin, lots of redundancy among glycoside hydrolases, and most genes are of unknown function 🙄.
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@ValdiviaLab
Valdivia Lab
3 years
Using FITC-labeled mucin we watched the process in live cells: it is selective, dynamic, and involves the formation of mucin-rich foci at the bacterial surface, pili, and eventual accumulation in "mucinosomes" (blame @valdi001 for the term). Are these paryphoplasms? TBD. .
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@ValdiviaLab
Valdivia Lab
3 years
What did we learn? Akkermansia can internalize chunks of mucin glycans that accumulate in internal compartments not unlike what has been seen in marine Verrucomicrobiae that eat glycans. The genetics points to "selfish" eating with most of the processing occurring within bacteria.
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@ValdiviaLab
Valdivia Lab
3 years
Finally! The culmination of almost 5 yrs of work by the brave Akkermaniacs team @DukeMGM. @lauren282012 developed genetics for Akkermansia, an important member of the human microbiota that was until recently considered genetically intractable
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@ValdiviaLab
Valdivia Lab
4 years
And Akkermansia says: Screw it, pass me the cookies!.
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