Some light holiday reading for everyone!! 🎉 🎊 I’m thrilled to share our newest preprint investigating galectin-3 biology in tear fluid 👀, led by rock star graduate student @vchangchemistry !! https://t.co/kp2s7OtAyv

@vchangchemistry I’m incredibly proud of the team, especially @vchangchemistry for pushing glycoproteomics to address such complex biological questions. Tear fluid still has so many glycobiology secrets left to uncover... and we’re just getting started! Stay tuned for more updates to come! 😇 😆

@vchangchemistry This work opens the door to understanding how glycans, splicing, and protein assembly cooperatively shape immune and barrier function at the ocular surface. We envision this could help guide future therapeutic strategies in conditions like DED, where Gal-3 levels are dysregulated

@vchangchemistry Even more surprisingly, we show that lacritin multimerization enhances Gal-3 binding, revealing a new biochemical mechanism for regulating glycan-mediated interactions in the tear film.

@vchangchemistry Only certain lacritin O-glycoforms, specifically those bearing core 2 O-glycans, engage Gal-3, and this interaction is further tuned by which lacritin isoform is present and whether lacritin is monomeric or multimeric.

@vchangchemistry In doing so, we identified nearly 100 Gal-3–enriched proteins, many tied to immune defense and antimicrobial activity. But (to me!) the most exciting finding was the discovery that lacritin is a glycoform AND spliceoform-specific ligand for Gal-3

@vchangchemistry Galectin-3 (Gal-3) is well known for its roles in immunity, inflammation, and mucin crosslinking at the ocular surface, but what it actually does in circulating tears is currently unknown. Using a combination of methods, we mapped the tear-fluid Gal-3 interactome.

suggesting a potential protective role for GCPII inhibition during aging. Overall, we're excited by these results... and stay tuned for next steps, where we plan to investigate the role of glycosylation in these sAD models!

involved in inflammation, calcium and cAMP regulation, protein deneddylation, and synaptic maintenance. These effects were especially prominent in association cortices that are highly vulnerable in sAD (i.e., dIPFC, ERC), and less so in resilient regions of the brain (VRC)

These animals make excellent sporadic Alzheimer’s disease (sAD) models, as their pathophysiology matches humans a lot better than commonly used animals. Using region-specific proteomic analyses in the monkeys, we found that long-term GCPII inhibition with 2-MPPA reshaped pathways

I’m so excited to share our new preprint! 🎉spearheaded by Alexandra Steigmeyer! https://t.co/UQtwEJfLQ4 In this work, we investigated how chronic inhibition of glutamate-carboxypeptidase II (GCPII) influences the aging rhesus macaque brain.

Results Realized! Read about the findings from a Pilot Project that lays essential groundwork in developing an early diagnostic for #ovariancancer, led by @YaleChem's Dr. @StacyMalaker. https://t.co/8OosOYtwAU #womenshealthresearch

Online now @an_chem ! Congrats to Shane Finn, @keira_erol, and the rest of the team! 🥳🥳🎆🎉 https://t.co/aDmgOvUQGt. Also, thanks to the reviewers who helped make this work even better, we appreciate you!

1/2 The fall issue of Innovations in Women’s Health is now available! Inside, we welcome Dr. Basmah Safdar as director, highlight new research, celebrate results, underscore the power of investing in women’s health, and introduce our Undergraduate Fellows. https://t.co/Sj0KwbuTZB

Congratulations to Professor @StacyMalaker on receiving the @acs_carb 2026 David Y. Gin Award in recognition of her exceptional advances in the emerging field of mucin glycoproteomics! She will receive the award and present her work at the ACS conference in the spring.

Today concludes #OvarianCancerAwareness month 🩵 but the work never stops. In 2023, @YaleChem Professor @StacyMalaker began a @WHRYale Pilot Project to better understand CA125, an #ovariancancer #biomarker. Read about her results in the Fall Newsletter, available mid-October! 📰

Congratulations Prof. @StacyMalaker on the @OnoInitiative award for her project, “Harnessing chemical glycobiology to target disease-associated glycosylation” – an investment in research projects with the potential to lead to discoveries in breakthrough treatments for patients.

Congratulations and welcome to Yale, @PetarPajic93 , who recently received an NSF fellowship to study the evolution of mucus and its role in pregnancy. He recently joined @StacyMalaker 's Lab as a postdoctoral fellow. Learn more https://t.co/uomktMbYqJ

@UvaChemPubs @molcellprot @keira_erol Here's to you, Don, and all the Hunt labbers!

@UvaChemPubs @molcellprot We also detail most recent methods used in their isolation and sequencing (primarily performed by @keira_erol ). We hope that this paper serves as both a resource to the community and a testament to the many (MANY) people that have worked on this project over the years.