Dan Shiwarski, Ph.D.
@ShiwarskiLab
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STEL lab Focuses on Bioengineering and Vascular Medicine at University of Pittsburgh SSoE and SoM FRESH Bioprinting | Biomechanics | Vascular Biology | GPCRs
Univ. of Pitt BioE and VMI
Joined December 2011
Integrating #FRESH #3Dbioprinting, #RegenMed, and #microfluidics we engineer platforms to study 3D tissue structure, extracellular matrix composition, biomechanical forces, and altered receptor signaling driving vascular tissue maturation and hypertensive disease pathology.
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First paper from @ShiwarskiLab at @PittVMI & @PittBioE in @PittDeptofMed and @Andrew_Hudson3D in Feinberg group @CMUEngineering on the cover of @ScienceAdvances! Check out our #FRESH #Bioprinting of perfusable scaffolds pancreatic tissue scaffolds.
science.org
Bioreactor maturation of high-resolution FRESH 3D-bioprinted scaffolds form vascularized centimeter-scale functional tissues.
Researchers have 3D printed pancreatic tissues that can sense glucose and release insulin, potentially transforming treatments for type 1 diabetes and human organ manufacturing. Learn more in this week’s issue of Science Advances:
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RT @ScienceAdvances: Researchers have 3D printed pancreatic tissues that can sense glucose and release insulin, potentially transforming tr….
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Check out our newly published work by @Andrew_Hudson3D and the Feinberg Lab to Enhance Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Microparticles @ACSBiomaterials @PittVMI @PittBioE
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Excited to attend @SFBiomaterials 2025 in Chicago. Come and see @ShiwarskiLab members present on advanced 3D biofabrication for Kindey and Lung disease @PittVMI @PittBioE #biomaterials
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Very fortunate and thankful to be selected for an @AdditionalVent Expansion Award to study “Fontan-Associated Portal Vein Hypertension and Fibrosis in a 3D Bioprinted Hepatic Sinusoidal Vascular System” with @isabelmjoyce and Dr Anita Saraf. @PittVMI @PittDeptofMed @PittBioE
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Excited to share excellent work by @MossSamuelP and @RegenBio on FRESH 3D Bioprinting of Collagen Types I, II, and III for cell scaffold fabrication | ACS Bio Sci & Eng #bioprinting #FRESH @cmu_bme
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Day 2 at #TERMIS2024 @antoni_tavares6 had his first ever Poster presentation (3DP resistance vessels) and got to hear exciting unpublished work from @RegenBio by @esgisss (Pancreatic 3DP) @CanerDikyol (LightPipe) and Ali (Liver 3DP). @PittVMI @PittBioE @cmu_bme #FRESH
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#TERMIS2024 Off to a great start with @bdcoffin’s talk about FRESH bioprinting Kidney models for proximal tubule physiology for our collaborate project with @caryboyd3. @PittDeptofMed @PittVMI @PittBioE
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Excited to be attending @2024wbc in Daegu S. Korea. What an opening ceremony and beautiful location. Catch our labs talk on building low-cost open-source bioprinters for high resolution tissue fabrication: SP-T02-0359 Thursday 9:55 - 10:10 am Room 321-A
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RT @caryboyd3: Cool article in Pitt Magazine about our studies with WNK kinases and biomolecular condensates. @ShiwarskiLab @arohan_s.
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@caryboyd3 is an inspiration to all by balancing clinical @PITTRenal, research, and family responsibilities and the perfect role model for our daughters. I am excited for our new collaborations on 3D models of proximal tubules and look forward to the future.
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From our first collaboration with @duvvuri_md studying #ENaC and #TMEM16A, to discovering #WNK1 #MolecularCondensates with @arohan_s and publishing our co-1st author paper in @CellCellPress. @caryboyd3 continues to be by best friend, closest colleague, and toughest editor.
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None of this would have been possible without help from our families over the years (Shiwarski’s and Boyd’s) and my partner, collaborator, and wife @caryboyd3.
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There have been countless friendships and collaborations between @CohenKarniLab @j_tashman @Andrew_Hudson3D @7JHinton @faazash @HijackSciJac @MossSamuelP @esgisss to name a few that have molded my career path and matured me as a scientist.
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Under Adam’s guidance @RegenBio I learned to apply engineering principles to challenges in biomedicine as well as mentoring, grantsmanship, leadership skills. He instilled the value of disseminating our work via build a Bioprinter workshops and #opensource hardware/software.
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In 2016 I took a huge step outside of my comfort zone into @RegenBio lab with Adam Feinberg @cmu_bme. Working on #biomechanics and #FRESH #3Dbioprinting we pioneered new tools and #opensource platforms for #tissueengineering and #RegenMed.
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Manoj pushed me to be better, confident, and step outside of my comfort zone. It was an amazing group @weinberz @FishFowlFriends @micro_mandy, ShannaBowman and a supportive and inclusive team. In addition to a great education in #Microscopy #SynBio #GPCRTrafficking
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In 2011 I joined @MPuthenveedu lab @CMU_Bio for my PhD studying the #CellBio of #GPCRs and delta #Opioid #ReceptorTrafficking in chronic pain. Manoj and @Shoba_Bio support, career advice, and friendship has been both a required and sufficient part of my professional career.
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My research is defined by great mentors. In 2009 @duvvuri_md in @PittHeadAndNeck we defined #TMEM16A role in cancer and metastasis. Uma pushed me to grow as scientist and molecular biologist always striving to improve patient health via basic research and clinical translation.
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