Shannon Rhoads
@ShanRhoads
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PhD Candidate at UNC, Chapel Hill 🔬🧠👩🏻🔬 Looking at #organelles during #neurodegeneration with #multispectral #microscopy #WomenInSTEM
North Carolina, USA
Joined April 2020
RT @jpflores_31: Nominations are open for the Index of Inspiring Black Scientists! Help us build a global, searchable resource of inspiring….
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Come see me at #CellBio2024!! I’ll be presenting on our recent pre-print using imaging-omics to characterize organelle differences between neurons and astrocytes!.
Cohen lab will be well represented at #CellBio2024! If you love #Organelles, #MembraneContactSites, and/or #Neurons and #Astrocytes, please come say hello!
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RT @alsncchapter: Please read our latest newsletter in which we launch the 2024 Feast for Families program, this year our goal is over $10,….
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Wow, I love this and am glad the topic has been discussed within such a large group and compiled so nicely. Great work!! 🎉.
New from me and a huge cast of amazing bioimage analysts in @J_Cell_Sci - - "The crucial role of bioimage analysts in scientific research and publication" . Hop into 🧵to learn more! (1/x).
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A huge THANK YOU🎉 to co-authors at @UNC_SOM (@ValerieDon83999 @ChihHsuanHsu1 @MC_Zanellati @ToddCohen27 @cohenlaboratory and more); collaborators (@DataTecnica); @FishCsCells and UNC’s Hooker Imaging Core for expert microscopy advice; @NIH and @ChanZuckerberg for funding!!.11/11.
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Our dataset includes 173 Z stack and 99 timelapse multispectral images and is accompanied by a quantitative dataset of 1418 organelle signature metrics. The data are on BioImage Archive ( for further exploration, analysis and hypothesis generation. 10/11
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Neurons and astrocytes had distinct organelle responds to acute oxidative and ER stress. Neurons showed more robust responses through changes in organelle interactions. In most instances, neuron and astrocyte organelle signature metrics trended in opposite directions. 9/11
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Astrocytic lysosomes (💚) also interacted more with lipid droplets and peroxisomes (🩷) than in neurons. Interactions between these organelles are involved in lipid metabolism. 8/11
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Astrocyte (right) organelle signatures contained more lysosomes💚 that were clustered perinuclearly and interacted more frequently with Golgi🩷 than in neurons (left), indicating differences in metabolic programming between cell types. 7/11
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Mitochondria💚 interacted with ER and peroxisomes🩷 more in neurons than astrocytes. Timelapses revealed mito-perox interactions in neurons (left) are maintained for longer durations than in astrocytes (right). These interactions are integral to mitochondrial homeostasis. 6/11
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Neuron organelle signatures were higher in mitochondria volume and number than astrocytes, highlighting the relative importance of mitochondrial functions, such as oxidative phosphorylation, to neuronal homeostasis. 5/11
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Neurons (💜) and astrocytes (🩶) have distinct organelle signatures reflective of their specialized functions. A set of 234 organelle metrics was sufficient to discern between cell types (PCA). 4/11
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We used multispectral microscopy to collect Z-stack and timelapse images of live primary neurons and astrocytes containing six organelle labels. Images were linear unmixed and deconvolved prior to organelle segmentation and quantitative organelle signature analysis. 3/11
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#Neurons and #astrocytes are morphologically and functionally distinct brain cell types: neurons are specialized to facilitate long-range electrochemical communication while astrocytes have many homeostatic and regulatory functions. Left: neuron, Right: astrocyte.2/11
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Check out our preprint using imaging-omics to characterize the #OrganelleSignature - the morphology interactions & distribution of #ER❤️ #Lysosomes🩵 #Mitochondria💚 #Peroxisomes🧡 #Golgi💛 & #LipidDroplets🩷 - of live #neurons and #astrocytes!.🧵1/11.
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RT @aps_unc: APS PhD student Jovan Tormes-Vaquerano @JovanTV mentored high school students this summer in the WinSPIRE program seeking to s….
aps.unc.edu
WinSPIRE Program Shaping a Diverse Future in Science and Technology By Dave DeFusco In a world where science and technology increasingly shape our future, ensuring that diverse voices are represented...
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RT @cohenlaboratory: Cool story from @YoniMalis and @KoretHirschberg on the role of Rab1 in targeting proteins from the #EndoplasmicReticul….
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RT @janfunkey: How can machine learning help you to analyze your microscopy images?. Find out at the "Deep Learning for Microscopy Image An….
mbl.edu
The goal of this course is to familiarize researchers in the life sciences with state-of-the-art deep learning techniques for microscopy image analysis and to introduce them to tools and frameworks...
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RT @WinspireU: Thanks all who stopped by our booth on Saturday, and thanks to Morehead Planetarium for having us at their Science Expo! We….
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RT @cziscience: Multispectral imaging of 7 different organelles within living iPSC-derived neurons 🤯. @DeshmukhLaB, @cohenlaboratory + @sye….
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