Justin Savage
@SavageNeurosci
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Duke Neuro BS 2020 Duke Neuro PhD 2???
Joined June 2019
RT @IralaDolores: It is finally here! In this work we found that astrocytes control SST+ synapse formation and function by secreting NCAN C….
cell.com
The astrocyte-secreted molecules controlling inhibitory synapse formation remained elusive for over a decade. Irala et al. found that astrocyte-secreted neurocan C-terminal fragment controls SST+...
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RT @salkinstitute: The Astrocyte-Team (A-Team) invites you to try Synbot, an open-source image analysis software, developed by Eroglu's lab….
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Thanks so much to my coauthors Juan Ramirez, @wc82rish, @IralaDolores, and @c_eroglu as well as all of the people in the Eroglu lab and beyond that tested SynBot. This wouldn't have been possible without all their help.
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SynBot is an open-source program written in the ImageJ macro language, making it easy to modify within FIJI without extensive programming experience. This will allow users to customize the program to their experimental needs.
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SynBot is also rapidly reproducible since it records the threshold values used in its analysis and can then load these values to reanalyzed images.
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SynBot is openly available at our GitHub site and there are tutorials for using SynBot and the experiments described in the paper at
protocols.io
Protocols related to the paper SynBot: An open-source image analysis software for automated quantification of synapses. Includes details of rat neuron and astrocyte culture, in...
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We tested SynBot in analyzing synapses in cultured neurons and in mouse brain tissue sections. SynBot performed equally well when compared to previous methods, required less user input, and recorded more data from each image.
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SynBot automates many of the steps of this analysis and can be combined with the machine-learning program ilastik for fully-automated analyses.
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New preprint alert!🚨 We present SynBot, an image analysis tool for quantifying synaptic colocalization in fluorescence microscopy images.
biorxiv.org
The formation of precise numbers of neuronal connections, known as synapses, is crucial for brain function. Therefore, synaptogenesis mechanisms have been one of the main focuses of cellular and...
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RT @PlanetofFinks: I'm not saying chiropractors should be illegal, I'm just saying they should have to have cigarette style warning labels….
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Super proud of my undergrad Medy Mu who presented a great poster on her work in the summer neuroscience program from @DukeNeuroMajor. Congrats to all the summer students who presented, it was one of the most interesting poster sessions I've been to.
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I convinced some really smart people to let me keep doing experiments.
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RT @c_eroglu: Please RT: The Eroglu lab is hiring. We are looking for two motivated individuals for two postbac RT1 positions. The first po….
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RT @YaruiDiao: Finally, our HiCAR paper is online in @MolecularCell! Thanks to all the team members for their hard work, as well as the sup….
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RT @napari_imaging: 📣 Excited to announce 0.4.13 release of napari! 🎉. Thanks to 28 authors, 30 reviewers, and more who tested our release….
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RT @DukeGenomics: We are so excited to host our first annual Precision Genomics Collaboratory Symposium on Dec. 3! #Duke grad students, pos….
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