(1/n) Can we build "perceptive" machines from mechanical parts? How "intelligent" were animals prior to evolution of nervous system? When does a collection of cells really become an organism? How can animal without muscles or neurons coordinate activity across millions of cells?
A lot happened since last post. Will write longer thread tonight - but quick update on "reusable PPE based on Snorkel". Devices are ready & being tested in the clinics today. This is the key Go-NoGo test. Will keep folks updated! All project details here:
(1/n)Excited to announce new course I have been planning for 5 years BioE271: Frugal Science structured as global participatory class open to all. 50% Stanford students 50% global participant. Learn how to solve serious challenges with little bit of play
(1/n) Latest pre-print from lab! Octopi; open configurable high-throughput imaging platform. Led by an incredible graduate student
@hongquan_li
- we crack puzzle of automated low-cost malaria diagnosis. Remember, malaria still kills ~1/2million people/year
(1/3) COVID situation in India is extremely serious. Many of us who have been working on pragmatic solutions for COVID have gathered as a volunteer taskforce - goal help & support wherever we can. The time for global community to engage & support India is now.
Got grant reviews: “Not Discussed” - Mustered courage to read. In short it said what we do is “esoteric” & irrelevant to broader science community. Words hurt! But it also reminded me of exact same reviews for what we did 10 years ago (Foldscope)! We pick ourselves and carry on!
Finally it’s arrived! Foldscope 2.0. We have been working on this for many years - so thrilled to see this out! Will write a longer thread on new features shortly - but finally we have swappable lenses (3!!). Your support allows us to bring microscopy to all corners of the world.
Introducing Foldscope 2.0 — available NOW! 🥳
Everything you love about Foldscope with new and improved features: 3 magnification lenses, ultra-simplified assembly instructions, and improved functionality!
#launch
#outreach
#stem
#scicomm
#education
It’s been an insane last two weeks seeing events unfold! But the only way to come out of this situation is “science the shit out of it”.. so let’s all do that. This moment will define open science for centuries to come! Short summary of what we are up to; what we need help.(1/n)
(1/n) Thrilled to share latest pre-print from lab - open imaging tool we have been working for the last 3 years "PlanktonScope V1 & 2" - modular (100x cost reduction) platform to enable citizens/sailors to study the world's largest habitat - our oceans!
1/n Latest pre-print from lab - Have you wondered how millions of cilia in your airway work together to generate coherent flow? This work was entirely led by an incredible postdoc
@guille_rochelle
in partnership with another multi-talented postdoc
@AJTM_M
In COVID19 crisis, health care workers globally need upward of 80M+ N95 grade masks. N95 masks are fibrous nano-materials made via melt-blown; large scale factory can only make 1M mask/day. What if another solution existed to this problem - Introducing Project 1000x1000 (1/n)
[1/n] "Oxygen" - If you know anyone in India - it's one word you hear over & over. It's a critical life saving supply. In context of COVID surge in India, we are sharing open source design for "pulse dose" O2 conservation device - potential to save 50% O2. Not medical device yet.
(1/n)Ever wondered how cells with intricate architecture survive "mechanical gymnastics" they seamlessly perform in living world - mind-bending extreme movements. Story features entangled organelles (ER), energy dissipation & discovery of "topological damping" inside a giant cell
(1/n) Latest pre-print. Can an adult living animal "shape shift" or morph right in front of your eyes? Led by incredible post-doc
@Viveknprakash
& brilliant theory graduate student Matthew Storm Bull Come for the movies; stay for "fracture mechanics"!
In the middle of acute oxygen shortage in India (likely be true for South-Asia); we are trying to respond by releasing open source solutions to conserve 50% O2 for treatment of mild COVID patients. Sharing Project OxSave - a passive solution to the problem
Sorry to post urgent request! Is anyone in the BayArea flying to India tomorrow & willing to take medical equipment for my dad 🥺Shipping bottlenecks are disaster. On organizational front
@IndiaCOVIDSOS
- we have supplies ready to leave but stuck w flight cancellation. Please DM
We opened our lab doors exactly 7 years ago, today. Just got news that we get to keep the doors open for another decade. This is a toast to every one of you who has passed through these doors, shaped and molded us. For all the countless inspiring, curious moments. Thank you.
Honored to be starting 2024 with community science in Peru. Lots of projects across Peru with partners from the Andes mountain to the jungle.
@TeamFoldscope
🌟 ¡Participa en la charla "FRUGAL INNOVATION: Do more with less" con Manu Prakash, catedrático de bioingeniería de Stanford University! 🚀✨
🗓️ 04 de enero a las 10 am en el Auditorio LID - Campus Central UPCH.
📌 Regístrate:
[1/n] 10 days ago we first shared blueprint of O2 conservation devices in context of
#OxygenShortage
; today we are releasing "Anmo2l" (means priceless in Hindi) - a ready to scale open design capable of saving 50-70% oxygen during O2 therapy. See details.
The second COVID wave is unfolding as a tragedy in India. I don’t need to convince anyone - all of us have family members in hospitals or seeking care. We need more global engagement and support NOW. Please join us at
@IndiaCOVIDSOS
- we can all help. DM me,
@paimadhu
@nitinaneo
(1/n) What does cotton-candy machine, van de graaff generator & COVID19 have in common? Here we present a possible solution for distributed, small scale manufacturing of N95 material for communities that can not source pre-approved masks. Pre print:
(1/n) Excited to share
#Pufferfish
an open-source full-feature mechanical ventilator reference design capable of volume & pressure control with assist modes, both invasive/non-invasive ventilation & features necessary to support COVID19 patients
Excited to share latest pre-print from our lab in collaboration with
@BhamlaLab
"Hydrodynamic trigger waves". Ever wondered if individual cells can talk to each other via hydrodynamic signals - wonder no more. Want to see a hydrodynamic antenna?
(1/n) Announcement: Just like every Jan, I will be teaching "Frugal Science" a global collaborative course focused on skills to tackle challenges in global health, scienceEd & climate action (new).
I have been away from interweb; working on scalable lowcost home diagnostic test. We are just starting the validation/feasibility study; & . Has been incredible partnership with Stanford Catalyst
@euanashley
& industrial partner BIC
(1/n) “Period poverty” defined as lack of sanitary products & toilets to more than 500million women is a key roadblock in providing gender equity worldwide. Can sustainable, distributed, local, open-source bio-manufacturing help? Read our latest pre-print:
Finally here - a fresh look at what a biology textbook should be - a guide to an explorer within you. This is an incredible effort by
@ibiology
in collaboration with TNQ technologies - led by Ron Vale & others. Oh, did I mention, it’s FREE for everyone!
Just sent message to 700+ students who have signed up from all corners of the world! Reading applications from everyone reminds me why we all became teachers. We have 10 weeks - 700 students - 30+ global mentors! And world littered with problems. Let’s go!
(1/n)Excited to announce new course I have been planning for 5 years BioE271: Frugal Science structured as global participatory class open to all. 50% Stanford students 50% global participant. Learn how to solve serious challenges with little bit of play
(2/n)This simple electricity-free LAMP protocol builds on fantastic work by Cepko lab at Harvard [], but with ahand powered centrifuge to make this assay completely electricity free for resource constrained settings. All you need is everything in this pic.
Latest lab pre-print: Excited to share yet another strange beast from our zoo - Emergent hunting mechanics of Lacrymaria olor - an incredible ciliate. Phenomenal work by Scott Coyle - postdoc in lab leading this effort.
Wonderful
@nature
editorial covers our recent open approach for distributed manufacturing of menstrual pads. Editorial highlights an argument I have been making for last 10 years “low cost” can mean high impact when done thoughtfully & with key partners.
An open-source process for turning fibers from the sisal plant into absorbent material for menstrual pads creates an opportunity for the local, sustainable manufacture of hygiene products that many communities need.
@PrakashLab
(5/n) This work started from a flash light I picked up from a toy store that was closing. My toddlers were kind enough to let me steal their toy which Ethan,
@anesta_k
and I in the lab hacked to build centrifuge. Toddlers at home still ask what happened to the little pig!
My lab with and without immigrants. This does not even begin to account for innumerable ideas that arose from diverse conversations that happened amongst us! Science infrastructure is not the buildings we work in, but it is the people that breathe life in science.
I enjoyed talking to
@srijanapiya17
at
@TimesEvoke
on “preserving our curiosity and playfulness” even in trying and difficult times. My views on “science for all”..
(1/n) Ever wondered if 3D printers have a role to play in open access health technologies? Curious how many cells could you image in a drop (2ul) of blood. Read about our latest frugal tool - Inkwell - an open-source tool for making perfect blood smears.
All the warning signs have been present all along. The international community needs to see this as a global crisis otherwise the world plunges into a new phase of the pandemic. Do what you can NOW - or we risk being in a forever pandemic. With
@paimadhu
(1/n) I love table top experiments. I love soap bubbles. So off course I absolutely love this paper - only wish the authors would cite the first "actual" experimental observation of branched flow of light by Yu Yu Stoilov starting 2002.
@nature
First visit to NCBS campus after coming here as a workshop participant 23 years ago
@NCBStheory
- this is the place that brought the seed of biological thinking to my door step as an undergrad. Incredible place to think big
#IBS2023
(1/n) 2023 begins with new hopes & dreams. Restarting our
#FrugalScience
global adventure this year with an invitation to global cohort of problem solvers. Here is invitation for global community to engage - come solve hard problems with us with a little bit of play.
A story of chaos as a driving principle for control of cellular behavior in a protist. Read our paper if you want to see a dynamical system approach to emergence of behavior in a non-neuronal system from the ground up. Also thrilled we got the cover! Congrats
@deepak_me90
Really excited to share that our work on understanding Lacrymaria olor behavior through the lens of active filament dynamics is now published
@PNASNews
. Dive in to see what connects protist behavior, active matter physics and chaos
Life in the last few weeks has been insanely busy. We have been working on an open-source mechanical ventilator capable of supporting COVID19 patients for 2 weeks duration. Delighted to share Utah-Stanford Ventilator Project (see )
(1/n) What is common between Silk Road, Klein bottle and a human parasite? Continuing series on extreme biological physics - thrilled to share latest preprint on "Extreme cellular hydraulics” - fantastic collaboration with
@BhabhaEkiertLab
at NYU Langone.
Secondly we just shared open designs and current ongoing efforts for how to covert your snorkeling full face mask into a reusable PPE for health care workers! The whole system being autoclavable. (5/n)
A new species of cynobacteria first isolated and identified with a
#Foldscope
from open ends of PVC plastic pipes of Municipality, Pune, India! 😳 - Congrats Dr. Kirti. Thrill of discovery, uncovering novel life forms under our nose.. what's not to like.
Big day for us - first steps for our saliva based COVID19 home test kit. The very first batch of units went out for distribution to our study participants. If you would like to engage and support; please see - proud of such an incredible team.
I have been away from interweb; working on scalable lowcost home diagnostic test. We are just starting the validation/feasibility study; & . Has been incredible partnership with Stanford Catalyst
@euanashley
& industrial partner BIC
From humble beginnings - Foldscope family has grown tremendously over last 5 yrs (1 Million users). This is story of Eden center & Mo Pandirajan
@MMopatnsf
who has trained nearly 100,000 kids in India, almost single handedly. Let that sink in for 1 sec
Congratulations Dr Deepak
@deepak_me90
- an end of an era. Hard to summarize everything you accomplished in the lab in last 5 years. So thrilled for a bright future for you ahead. It was honor to recreate hooding ceremony - & start new tradition of decorated graduation hats!
The global coalition IndiaCovidSOS is in place with 100+ academics, clinicians, bioengineers, community members working 24/7. This is first output from group; our goal is to bring clinically validated solutions and help. We need global community to engage - time is running out!
A very special day today - community distribution for our usability study - it’s been long road designing/assembling/testing & sharing this with community. So thankful for amazing team - with the goal to bring low cost tests to communities around the world
(1/n) Thrilled to share latest invention from our lab - a whole new way of vertical tracking microscope -capable of imaging single cells & marine larvae swimming as if in unconfined ocean? Read pre-print. Congratulations
@deepak_me90
&
#GravityMachine
team
Inspired by
@amy_harmon
from last year; I am handing out
@TeamFoldscope
#Foldscope
instead of candy today! So I had to dress up as a box of Foldscope. Happy Halloween.. may the spooky (for real) world of microcosmos haunt you.
(1/n) In case you still want to make some Diwali Rangoli - and want to explore a new kind of "topological trap" in spiral active matter - enjoy this hypnotic thread from latest story from the lab
#DFD2023
Read details here:
So honored to be in company of amazing, sometimes silly yet impactful inventions. This award is for the global
#foldscope
community that inspires me, surprises me and shares the joy of science with the next generation.
@TeamFoldscope
@stanford
Thank you. 🙏🏾
Congratulations to 2022 awardees Manu Prakash and Jim Cybulski
@TeamFoldscope
. Applying their principles of ‘frugal science,’ they developed a paper microscope, known as a Foldscope, with the goal of expanding global access to science. Read more:
#GGA22
1200 students engaged in a single
#foldscope
workshop focusing on a rural region of the country! Kudos to the
#foldscope
fellows/trainers. Happy children’s Đạy.
2day was even bigger crowd, students from nearby schools also came. Total of 1200 students made their Foldscopes n learnt to use it. Tomorrow we all will go on bio-walk n use Foldscope to observe. Looking forward to it.
#rajaborari
#scicomm
#sciencetwitter
#science
We will continue to have new COVID variants occur and circulate. We must build global policy to share critical resources in short supply like vaccinations and diagnostics in areas of highest need globally. It’s a pandemic; we must fight it like a pandemic. By global cooperation!
Vietnam has detected a new coronavirus variant that is a combination of the Indian and UK COVID-19 variants. The country's health minister said it spreads quickly by air and described the hybrid variant as very dangerous
(3/3) Will post specific ways you can help soon. Key priorities philanthropic donations, home care, diagnostics, oxygen/ventilators, training, sequencing, science communication &more. Includes
@paimadhu
@nitinaneo
@KrutikaKuppalli
Nitika TomBaer,
@tifrarnab
@mlipnick
& many more.
(1/n) After an incredible last season of "
#FrugalScience
BioE271" class from last year (); 490 students from 58 countries - I am again teaching my fav class this quarter. As always - it's free and open to all - anyone around the world can apply.
Delighted to share
#BiteOscope
is finally out in
@eLife
- has been a long time in the making. We are excited to help others use this tool for exciting discoveries. More on that soon. Led by amazing postdoc
@felixhol
now starting his own lab. thread below.
Want to learn what mosquitoes do when they bite - without sacrificing arm & leg? Latest story from the lab - BiteOscope - open platform for quantitative mosquito behavior. Led by
@felixhol
- who was a postdoc in the lab - now starting his own lab at CRI
(10/n) We urgently need coherent global strategies instead of every country/state/county for itself. We need to re-engineer supply chain. Bring distributed manufacturing to the table. Share solutions and IP openly. Actually follow public health policy and scientific guidelines.
Preparing for Halloween 🎃 giveaways - as is tradition, we give
#Foldscope
instead of candy to truly let the kids see all the scary “tiny” monsters of the microscopic world! Join in the tradition started by
@amymaxmen
and friends a few years ago!
@mbeisen
Wonderful story covering our discovery of new materials for sustainable manufacturing of menstrual pads is covered in India
@the_hindu
- we are excited to build a consortium of community partners tackling this problem collectively. Please see and engage!
(17/n) Remembering life events 8 years ago is hard - but I explicitly remember the night we imaged these animals & made an incredibly beautiful yet perplexing observation - thousands of cilia appeared to "flock" like birds. Matt and I often talk about that day.
What we saw unfolding in india has now begun ravaging Uganda. And this will continue until vaccines are equitably available! I am struggling to make sense of the world these days. We learned a lot with India’s 2nd wave - those lessons should be broadly applicable.
@IndiaCOVIDSOS
We are dying! Ugandans need the world to stand with them. We need oxygen, PPEs,
#COVID19
vaccines and money to pay our over-stretched health workers.
World, we need each other!
Oxygen failure kills 30 Covid patients at Mulago via
@DailyMonitor
(2/3) We are a group of passionate clinicians/epidemiologists/engineers/public health/policy/students and on the ground community members spread across the world. Please tag and share similar efforts so we can coordinate - if you would like to engage, please DM/post here.
(7/n) We are releasing "handyfuge" files as open hardware CERN-OHL-W license. To build handyfuge, get yourself a $5 flash light and download laser cutter files at GitHub page:
(1/n) Doing science - we also make memories & stories. Some stories are told & some stay with us. They don’t have to be consequential - just they tickle us each in special way. For this
#PostdocAppreciationWeek
- sharing our appreciation of wonderful, young, bold scientists..
10yrs ago - we dreamed of sharing live microscopy images w large groups. That was seed of “Projection Microscopy” 📽️+ 🔬= ❤️ So happy we can finally release it in product form.
@TeamFoldscope
Kickstarter campaign. If you can - please support our efforts to democratize science.
Introducing The MicroRealms Projector! 🎉 A powerful and affordable 50-1000X microscope with an embedded projector, small enough to fit in your pocket.
Live on Kickstarter - get yours now:
#newproduct
#launch
#microscopy
#stem
Pandemic isn’t over. It’s transforming & rising in places with limited resources! If we can’t band together to support countries in need; it’s not just a moral failure - it also brings new variants to everyone’s shore. First India, Nepal, Uganda & now Kenya..
@IndiaCOVIDSOS
Just like you can check out a book from a library; now you can check out a
#Foldscope
. I know several public libraries in US keeping Foldscope. Would love to see more libraries participate. For some who know my history with library fines - this gives new meaning to a lost book!
Foldscope instruments are now available in our STEAM Lending Library. Contact James
@TheEducationPartnership
.org if you are interested and click the link to learn how to use kits.
The fact that it’s 2021; and trajectory of science and scientists is dictated by bureaucracy of getting visa extended is incomprehensible. Being an immigrant; I know this is far too common from personal experience. Indeed a loss for US and gain for Switzerland.
I closed shop today. It has been a wild ride
@Dunn_Lab
@Stanford
. I will be broadcasting soon from Switzerland. My future is uncertain. This move was not planned but I will face the new challenge. If you know of any job for a scientist that like flower’s shirts, let me know.
Just landed in India for
@iisfofficial
Foldscope workshops in partnership with
@DBTIndia
- ~4000 kids/teachers coming from all across the country. Volunteers arriving to help from all parts. Possibly the largest Foldscope workshop in the world! Wish us luck
@TeamFoldscope
Teachers shape our thinking in so many ways; but we often don’t get to share how we embody what we learned & pass it on. Just saw this beautiful fictional short film by
#Foldscope
user (Mayank Garg) & it brought a tear in my eye. Happy Teachers day!
Thrilled to announce that we are now accepting project proposals from
#frugalscience
teams - supported by platform and Schmidt Futures. See details here - Thanks
@davidtlang
for these resources.
(1/n) Announcement: Just like every Jan, I will be teaching "Frugal Science" a global collaborative course focused on skills to tackle challenges in global health, scienceEd & climate action (new).
(19/n) Next we took inspiration from what is known about information propagation in bird flocks. Accidentally,
@Viveknprakash
& I had already collected tons of bead data for our fracture studies - which Matt could use to directly compare single cell motility in an entire animal.
Some long overdue lab celebrations/announcements; thrilled to share that
@GraceJZhong
BioE graduate student in lab passed her PhD quals in flying colors. Her joy and enthusiasm for science captured in this rare moment
@GraceJZhong
finding her first wild Tplex (prior to shutdown).
Thrilled to share
@hongquan_li
successful PhD defense - latest doctor from the lab. Hongquan has been the driving force for much of our malaria diagnostics (
#octopi
) & open tools for life sciences (
#squid
) communities. More news on this very soon as we scale both these projects.
What to light up your Christmas holiday 🎄- enjoy building this beautiful implementation of fluorescence microscopy (TIRF adaptor) on a
#foldscope
. Opens a lot of fun new experiments - will test this soon. Congrats Sangeeta and Jitendra
@himdaughter
@kvijayraghavan
7 yrs ago, when we first sketched a
#Foldscope
on a piece of paper - we only had a bare glimpse of where we will be. Microcosmos post from Moovanthan.P et al working with tribal women farmers, Chattisgarh, India - demonstrates the power of inclusion.
@himdaughter
@PrinSciAdvGoI
(8/n) Thanks again for everyone in scientific community that has been sharing results rapidly. The whole lab has been under a lot of stress to get tools & solutions to communities world wide. I will try to put structured updates here
(4/n) We have been developing many frugal tools for applications in diagnostics - including other hand held centrifugation techniques (
#paperfuge
) for many years. The advantage for handyfuge is increased stability and limited fluctuations in RPM and it matches covid test need.
(6/n) The goal was always to combine experiments and theory - to deeply understand "tapestry of nature". We took an approach of "model by construction" & start from the very basic unit of motility - single cilia - & scale all the way to a complete animal with millions of cells.
Out this week; our review on “multi scale physics of cilia” with focus on where we believe the next set of puzzles lie in a fascinating wonderland of cilia, cellular motility and its control. With lab members
@wgilpin0
and Matt Storm Bull.
Review: The multiscale physics of cilia and flagella. Cilia are hair-like organelles that enable cellular locomotion. William Gilpin, Matthew Storm Bull & Manu Prakash
@PrakashLab
discuss cilia from a dynamical systems perspective
Read it now:
Excited about organizing this meeting with some of my favorite scientists. Really looking forward for us as a community to be bold and ambitious about discuss big unanswered questions. Come for the biology, theory and stay for the philosophy and biodiversity.
#4DCPJanelia
Join us! Our 9th
#4DCPJanelia
workshop explores general principles of
#multicellular
life and the fundamental differences between single cells and multicellular organisms.
Students + postdocs👉Apply by Oct 1 for a short talk
@HHMIJanelia
@HHMINEWS
(8/n) This journey began when we came up with a clever trick to image a live animal in cross-section - to our surprise; the animal "walks" with cilia. This is unique since ciliary dynamics have not been studied with constrain. We asked can cilia naturally generate a walking gait?
Delighted to introduce a new doctor in town - Dr. Anton Molina! Anton defended his PhD from the lab in Material Science. The range of work covered during his Phd from "Droplet Ising machines" to "viral factories" and new solutions for "Period poverty" is breathtaking. Congrats!
Working with young scientists always reminds us why we all started this journey in science. Thanks
@AshokaUniv
@AmbikaAnupama
@kvijayraghavan
and entire team for building incredible program to bring curiosity driven science to 9-12th grade students across India
@TeamFoldscope
An Unforgettable Field Trip!
Students had the incredible opportunity to embark on a captivating field trip where they delved into the hidden marvels of the microscopic realm using Foldscopes.
#FieldTripFun
#LodhaGeniusProgramme
@Lodha_Group_
I often feel the capacity to uncover secrets of nature lies within our ability to observe. Tools help guide the way. Beautiful book by
@ragh_gadagkar
an incredible lesson on power of low-cost well designed exp.
#foldscope
community pursues this approach for behavior of microbes.
An amazing meeting focused on imaging in marine organisms - at one of my favorite marine station.. literally surrounded by history.
#EMBOmarineimaging
with Ellie and Hannah from the lab.
(2/n) With an incredibly amazing graduate student in my lab - Matt Storm Bull - we went on a quest that almost lasted 7 years. This is a multi-scale story in 3 parts (arXiv ); subcellular, tissue and organism scale. What an amazing privilege to go on this expedition with you Matt
(3/n) (1)Excitable mechanics embodied in a walking cilium
(2) Ciliary flocking and emergent instabilities enable collective agility.. (3) Mobile defects .. shape the locomotive behavior of a headless animal
We have a lab tradition to do yearly recaps - in a goofy way - to reflect/celebrate the humanity behind all the science. A little bit of inner life of a Prakazoan. Kudos to Rebecca Konte in the lab for keeping this tradition alive. Sharing 2023 edition.