❄️ Enjoying the first and, hopefully, not the last, snow of this winter ❄️

Stay tuned for more insights into viral entry mechanisms! 🧫.If you liked this #tweetorial, please retweet the first tweet. Endosome rupture enables enteroviruses from the family Picornaviridae to infect cells.#CEITECScience.9/9.

𝗪𝗵𝘆 𝗱𝗼𝗲𝘀 𝘁𝗵𝗶𝘀 𝗺𝗮𝘁𝘁𝗲𝗿?.This study brings us closer to the fundamental understanding of the infection process. 8/9

𝗠𝗲𝗺𝗯𝗿𝗮𝗻𝗲 𝘄𝗮𝗿𝗽𝗶𝗻𝗴 𝗮𝗻𝗱 𝗲𝗻𝗱𝗼𝘀𝗼𝗺𝗲 𝗿𝘂𝗽𝘁𝘂𝗿𝗲.Actin filaments observed at membrane protrusions, along with actin polymerisation inhibition results suggest cellular actin can drive endosome rupture. 7/9

On the contrary, inhibition of endosome acidification didn’t stop genome-containing particles from exiting the endosomes. 6/9

𝗖𝗮𝗻 𝗰𝗲𝗹𝗹𝘂𝗹𝗮𝗿 𝗺𝗲𝗰𝗵𝗮𝗻𝗶𝘀𝗺𝘀 𝗯𝗲 𝗶𝗻𝘃𝗼𝗹𝘃𝗲𝗱? 🦠.Inhibition of the actin polymerisation led to genome release within the endosomes, hindering the virus replication. 5/9

𝗛𝗼𝘄 𝗱𝗶𝗱 𝘁𝗵𝗲𝘆 𝗱𝗼 𝗶𝘁?.Using advanced #cryoET @cemcof to visualise cell entry of rhinovirus 2, echovirus 18, echovirus 30, and enterovirus 71 – right in action! 🔎.4/9

𝗞𝗲𝘆 𝗙𝗶𝗻𝗱𝗶𝗻𝗴𝘀.This study reveals that enterovirus-containing #endosomes can deform and rupture, enabling the release of genome-containing particles into the cytoplasm. 3/9

#Enteroviruses, members of the Picornaviridae family, cause illnesses ranging from the common cold to encephalitis 😷 However, the infection process was never observed 𝘪𝘯 𝘷𝘪𝘷𝘰 - until now. 2/9

✨𝗡𝗘𝗪 𝗣𝗔𝗣𝗘𝗥 𝗔𝗟𝗘𝗥𝗧✨.We are happy to announce that the paper by Aygul Ishemgulova @IshemgulovaA and members of our group, describing the novel mechanism of cell invasion by enteroviruses is now published in @CommsBio! 🦠.Let’s break it down! .🧵 .1/9

Great news from Portugal! #phageTherapy.

RT @paulynamagana: #AlphaFold3 code & weights are now open-source for academics! 🎉.Looking forward to seeing what new research this unlocks….

Článek s rozhovorem na @Radiozurnal1:.

🇨🇿 Naše Dr. Lucie Valentová popsala svůj výzkum bakteriofága JBD30 pro @CRozhlas 📢. V rozhovoru se dozvíte, jakou novou, námi popsanou, strategii používá fág JDB30 na lov své kořisti - Pseudomonas aeruginosa 🦠. Odkaz níže👇

If you are interested, find the tweetorial and the link to her paper below 👇.

🎉 Congratulations to freshly minted doctor Lucie Valentová #PhDone 🎓. We wish you all the best in your next chapter—your passion and dedication will continue to inspire! 🌟. #PhD #PhDlife #NextChapter

12/12.Thanks to our amazing collaborators for making this work possible - kudos especially to @Ceitec_CellimCF and @cemcof !. #CEITECScience #cryoEM #virus #AntibioticResistance.

11/12.We’ve walked you through JBD30’s epic journey: from its flexible tail and high-tech docking system to taking over P. aeruginosa cells, assembling DNA-packed heads, and building the ultimate phage army. 🚀✨

10/12.Once inside, the phage takes control, turning the cell into a phage factory🏭.🛠️ Capsid proteins snap together into procapsids, which then expand - making room for the precious genome📦.🧩 With their heads full, they team up with the tails, creating the perfect phage squad!

9/12.The next move in JBD30’s screenplay: The tripod and baseplate spring open, unleashing three copies of the tape-measure protein! 📏.These proteins then unfold into what’s likely a trans-membrane channel—a perfect escape route for the DNA to make its grand exit.🚇