📢 Ready to rethink XIST? Our latest work, now in Cell, challenges two fundamental assumptions about XIST: its cis-limited action, and its role in equalizing gene expression between XX and XY genotypes. Dive in 👇🤿(1/6) .

RT @VashKurdistani: Excited to share our latest work: L126 fine-tunes enzymatic activity of histone H3, a discovery made possible by viewin….

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RT @andrea_cerase_: I'm delighted to have been mentioned in the celebratory article commemorating the 30th anniversary of NSMB #Xist_granul….

One of the most extensively studied lncRNAs continues to surprise us! A huge thanks to everyone who helped with this! @Iris_Dror @tsotnech @ClaraCanCanoe @Dr_Sahakyan @YolandaMarkaki @ChronisLab @Mandy_Collier @WeixianDeng @DingFangyuan, #lncRNA, #SexDifferences (6/6).

This raises the possibility that autosomal genes in other cell types with a dispersed XIST configuration (pre-implantation embryos, germ cells, immune, lung, and cancer cells) may be regulated by XIST. (5/6).

We made similar discoveries in differentiating mouse PSCs where Xist is in a dispersed configuration and autosomal localization also occurs, suggesting that dispersed configuration of XIST/Xist is coupled with autosomal spreading. (4/6).

Applying RAP-seq, we found that XIST spreads across the X and, surprisingly, also to autosomal genes. Autosomal targets show female-specific and XIST-dependent H3K27me3 and downregulation of gene expression, indicating that XIST enforces sex differences on autosomes. (3/6).

In female naïve human pluripotent stem cells (PSCs), XIST is dispersed in the nucleus and X-chromosome dampening (XCD) occurs, raising the question of whether XIST mediates XCD. Knocking out XIST, we found that it is required for XCD in naïve hPSCs. (2/6).

In collaboration with @dinodicarlo, we used hydrogel nanovials to capture and measure the secretion of VEGF-A alongside mRNA transcriptomes. Surprisingly, we found that the VEGFA transcript was not the best predictor of secretion! 2/2.

Check out our latest publication where we developed SEC-seq – a new technology that captures and measures single-cell protein secretion coupled with mRNA transcriptomes published in Nature Nanotechnology! 1/2.

Congratulations to Plath lab member @JustinLangerman who was awarded the UCLA 2023 Boyer/Parvin Postdoctoral Award. 🎉

RT @JonesLabUCSF: Join us for this incredible @GordonConf on #StemCells and #Cancer in beautiful #Tuscany organized with @CedricBlanpain an….

This work was led by @JustinLangerman and @shreya_udani with the @Dinodicarlo lab.

It's time to bring single cell secretion measurement into the multiomics family! Check out our recent BioRxiv submission where we found unexpected relationships between VEGF-A secretion, the VEGFA mRNA, and the cell state:

RT @NovitchLab: I’m happy to share our new paper with @LabMomoko published today which examines how differences in….

Congratulations to @YolandaMarkaki and all other award winners for the UCLA 2022 Boyer/Parvin Postdoctoral Award. Tune in for her seminar on "How to silence a chromosome" July 20th at 12PM PT. 🧬

RT @TTdevbio: It’s finally out! Many thanks to my colleagues @jacksongcheng and my mentor Andy McMahon at USC for making this happen. #orga….

Excited to share our recent review on the role of Xist in maintaining X inactivation!. By @Elsie_youlater and Amy Pandya-Jones.

RT @tsotnech: Happy to share our most recent paper (1/3):. FGFR3 is expressed by human primordial germ cells and is repressed after meiotic….

RT @sacnas_ucla: We are excited to host @DrPlattLab from @GeorgiaTech as part of our Scientific Excellence Through Diversity Seminar (SEDS)….