Stefan Pfeffer
@PfefferCryoLab
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Cryo-electron microscopy, structural biology of molecular machines, ribosome-associated processes
Heidelberg, Germany
Joined October 2020
Congratulations to the shared first authors @martin_wuertz and Giulia Tonon! Fantastic collaboration with the Schiebel Lab @ZmbhH, @FabioLolicato at the BZH Heidelberg, @EustermannLab @embl, and the Erhardt Lab at KIT Karlsruhe. Thanks to all who contributed!
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Nearly two decades after augmin's discovery in Drosophila, we have reconstituted the microtubule-binding region of the Drosophila augmin complex on microtubules and demonstrate the conserved mechanism by which the augmin subunit HAUS6 orients augmin for microtubule branching.
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We are excited to share our latest work elucidating the structural basis for how augmin binds to microtubules. Out just now @NatureComms: https://t.co/zfSenm6j8C
nature.com
Nature Communications - Augmin is essential for microtubule nucleation during cell division. This study reveals how the conserved calponin homology domain of the augmin subunit HAUS6 binds to...
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Great collaboration with the Melnikov Lab @SergeyVMelniko1 @UniofNewcastle and the Hill Lab @chillzaa @YBRI_UoY. Congratulations to all authors, in particular the shared first authors Karla Helena-Bueno and Sophie Kopetschke @S_Kopetschke.
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Out just now at @NatureComms: using cryo-EM and cryo-electron tomography, we provide evidence that structurally heterogeneous ribosomes can cooperate in protein synthesis in bacterial cells: https://t.co/Gg2qBHbL7W
nature.com
Nature Communications - Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that...
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1/2 Here we unexpectedly find that protein S20/L26—identified at the dawn of ribosome research but then deemed a ribosome isolation artifact—is indeed a genuine component of the large ribosomal subunit. More on this ribosome content variation:
nature.com
Nature Communications - Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that...
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A great collaboration with the Schiebel Lab @Zmbhh
@UniHeidelberg and the Gruss Lab @UniBonn. Congratulations to all authors, in particular the shared first authors Qi Gao, Florian Hofer, Bram Vermeulen @BramV98 and Sebastian Filbeck!
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Online today at @NatureComms: in a major team effort, we reveal the structural mechanisms for centrosomal recruitment and organization of the human microtubule nucleator γ-TuRC:
nature.com
Nature Communications - The γ-TuRC plays a central role for nucleation of microtubules at the centrosome. Here, the authors dissect structural mechanisms for centrosomal recruitment and...
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Great collaboration with the Schiebel and Mayer Labs @Zmbhh @UniHeidelberg. Congratulations to all authors, in particular the shared first authors Anjun Zheng, Bram Vermeulen @BramV98 and Martin Würtz @martin_wuertz.
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Out just now at @NatureComms and a great start into the New Year: we reveal how yeast cytoplasmic γ-TuRC is assembled and activated by dimeric CM1 motifs of the receptor protein Spc72 and how the microtubule polymerase Stu2 binds to promote MT assembly.
nature.com
Nature Communications - The γ-TuRC plays a key role in regulated nucleation of microtubules (MTs). Here, the authors reveal how yeast γ-TuRC is assembled and activated by dimeric CM1...
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Congratulations to Martin Würtz, who yesterday received the Nikon Young Scientist Award 2024 for excellent research in cell biology awarded by the German Society for Cell Biology.
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We have an open PhD position to explore structures of gamma-tubulin ring complexes using cryo-EM approaches here in our group @ZmbhH
@UniHeidelberg. Please apply via the Heidelberg Biosciences International Graduate School @HBIGS_HD :
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We still have open PhD positions here in our cryo-EM group @ZmbhH @UniHeidelberg. Please apply via the Heidelberg Biosciences International Graduate School or directly send us your application documents. https://t.co/cJZCFXm0hI
We have two open PhD positions to explore structures of ribosomes and gamma-tubulin complexes using cryo-EM approaches here in our group @ZmbhH @UniHeidelberg. Please apply via the Heidelberg Biosciences International Graduate School @HBIGS_HD:
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We have two open PhD positions to explore structures of ribosomes and gamma-tubulin complexes using cryo-EM approaches here in our group @ZmbhH @UniHeidelberg. Please apply via the Heidelberg Biosciences International Graduate School @HBIGS_HD:
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Great collaboration with the Schiebel Lab @Zmbhh @UniHeidelberg and the Gruss Lab @UniBonn. Congratulations to all authors, in particular the shared first authors Bram Vermeulen @BramV98, Anna Böhler and Qi Gao @QIGAO31314616.
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Out just now @embojournal: our cryo-EM structure of the native RanGTP-induced γ-TuRC-capped microtubule, revealing a surprisingly asymmetric minus end architecture with direct implications for microtubule regulation and modulation during spindle assembly. https://t.co/EqUpgynh9G
embopress.org
imageimageThe universal microtubule nucleator γ-TuRC assumes a microtubule-incompatible open conformation prior to nucleation. This work finds it transitioning to a partially closed conformation,...
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We still have ERC-funded PhD positions open in the lab! If you are interested, please apply!
We have three open PhD positions funded under our #ERCStG 'RiboStress' to explore how cells remodel the activity and molecular organization of the translation machinery in response to stress. If you are interested apply via the HBIGS graduate program:
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We have three open PhD positions funded under our #ERCStG 'RiboStress' to explore how cells remodel the activity and molecular organization of the translation machinery in response to stress. If you are interested apply via the HBIGS graduate program:
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If you want to read a short summary of the projects of the three new @ERC_Research Grantees #ERCStG Daniela Duarte Campos, Kerstin Göpfrich @KGoepfrich, and Stefan Pfeffer @PfefferCryoLab based @ZmbhH visit: https://t.co/l6h060jNN8
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Fünf ERC Starting Grants gehen an die Universität Heidelberg – Europäischer Forschungsrat fördert Vorhaben in der Molekularen Biologie, der Malaria-Forschung und der Astronomie mit insgesamt rund 7,5 Millionen Euro
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