Bonjour!.Let me introduce you to the citrus project🍊🍋 I am part of at #Yale. The citrus industry faces unprecedented challenges from the spread of the #CitrusGreening disease, #HLB. This is caused by a bacteria, infecting the tree’s phloem leading to its death . 🧵(1/3)

RT @YalePostdocAsso: Over the past two months, we’ve collected data on #postdoc compensation through a survey. Today, we’re here to share t….

Florida's citrus production has been in freefall since citrus greening was first detected in 2005. No cure for this disease that wreaked havoc in citrus orchards. Add to that the damage from hurricanes and the citrus farmers are under immense pressure. #AllInForCitrus

#NewPaper "An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus" in @PlantMethods is out!!!. 📓

5️⃣🧬Rapid Gene Editing: #CRISPR/Cas9 is used to introduce mutations in citrus, producing gene-edited plants within three months instead of 9-12 months in tissue culture. Don't forget to 👍, 🔄 and reach out with any questions!.

4️⃣🧫 Efficient Inoculation Techniques (transformation): The AGWC (apical bud cut, growth, wound, and cotton inoculation) and BCTI methods are the most effective, showing high regeneration and transformant shoots.

3️⃣🍊Critical Growth Conditions: High humidity and the use of younger seedlings (4-6 weeks) are key factors in achieving successful regeneration and transformation.

2️⃣🍊High Regeneration Rates: This new method increases the regeneration rate in lemon to over 95% in planta compared to 4.8% in #tissueculture.

1️⃣🍊Tissue Culture-Free Transformation: .We developed a in planta transformation method for recalcitrant citrus cultivars such as lemon and pineapple sweet orange, bypassing the need for labor-intensive tissue culture techniques.

🗞️ Have you seen our latest paper in @FrontiersIn - “Optimization of In Planta Methodology for Genome Editing and Transformation in Citrus”? 🍊 🧬 . Below are 5 key points about this new methodology:.🧵

Check out my recorded flash talk on how the Yale Citrus team “Combat the Citrus Greening Disease with Gene Editing” presented at the 2024 @MCDB_Yale Retreat! 🍊. After seeing it, would you come to my poster? Share your thoughts and feedback ⬇️

Having some fun in the lab on my special day! 🥳

Excited to have attended the 2024 MCDB Departmental Retreat! . I had the opportunity to give a 3-minute flash talk and present a poster on the Yale Citrus project – an amazing experience to share our progress with the department! 🍊 . #ScienceRetreat #CitrusResearch #yale

RT @YalePostdocAsso: ⭐️ Updated event calendar ⭐️. Get ready, Yale postdocs! . National Postdoc Appreciation Week (NPAW) is just around the….

The National Postdoc Appreciation Week (#NPAW24 ) is just around the corner! . Check out what the @YalePostdocAsso and OPA have prepared 🙌 .@nationalpostdoc .#NPAW.

Great feeling to know your growth room is ready for our heat stress protocol to increase the efficiency of #CRISPR /Cas9 and increase #genomeediting in Citrus. For the curious one, this is the protocol we follow <gt;

🔊 If you are interested in doing your postdoctoral training at #Yale, this link might help you find an opportunity: Many positions are available in various fields/Departments. Happy job hunting and share this to help others. #jobsearch #postdoctoral.

#Publication.Excited to see our paper published on the optimization of in planta transformation to bypass tissue culture and get high regeneration rates and efficient gene editing.

RT @YalePostdocAsso: 🚨 Attention: Postdocs reappointed before July 1st are getting paid less than the new minimum! This is not the case amo….

RT @YalePostdocAsso: 🗣️ Did you know?.@Yale Postdocs have less PTO than Postdocs at @Harvard and other Ivy Leagues?.Please retweet if you f….

If you are an incoming #Postdoctoral or #AssociateResearchScientist at Yale, please consider joining the association, we are here for you! Reach out directly to me or email ypa@yale.edu. #university #YPA.