Miguel Ramalho-Santos Lab
@MRSantosLab
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The Miguel Ramalho-Santos Lab does research on Developmental Epigenetics at the Lunenfeld-Tanenbaum Research Institute and the University of Toronto, Canada.
Toronto, Canada
Joined February 2018
Learn more about the lab's research and news at: https://t.co/JBDoaj85bn and https://t.co/rlNYMz8N8C
mrsantoslab.org
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The #LTRI in Toronto is a diverse + and inclusive community in a multicultural city: we encourage candidates from underrepresented groups to apply! 2 PI positions, by Oct 20. #womeninscience
#BlackandSTEM
#NativeinSTEM
#NativeScience
#LatinXinSTEM
#MarginSci
#DiversityinSTEM 👇
Job Alert 🚨 We are looking for outstanding candidates to join our institute! Find out more & apply: Biomedical Discovery ➡️ https://t.co/HOjgJmHio7 Population Health Data Science ➡️ https://t.co/qBl4JkKXcf
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🚨 JOB ALERT 🚨 We are hiring TWO principal investigators in systems, cellular, chemical or molecular biology in Toronto, Canada at @SinaiHealth. We provide a generous startup, fully funded salary and academic appointment at U of Toronto. Apply now or repost!
Job Alert 🚨 We are looking for outstanding candidates to join our institute! Find out more & apply: Biomedical Discovery ➡️ https://t.co/HOjgJmHio7 Population Health Data Science ➡️ https://t.co/qBl4JkKXcf
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Ending 2024 in style! We found that LINE1s are key “architects” of the nucleolar genome in human embryonic stem cells. This work brings together 2 facets of Barbara McClintock’s pioneering work: nucleolar organizer regions and transposable elements. https://t.co/2mZaMPtNuQ...
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Also noted: Ribosomal DNA copy number is associated with body mass in humans and other mammals.
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Discussed in @MRSantosLab lit review: Stem cell activity-coupled suppression of endogenous retrovirus governs adult tissue regeneration: Cell
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In a groundbreaking discovery, Sinai Health researchers have found that transposable DNA elements – long considered “junk DNA” because they had no known function – are critical for early human development. Congrats to Dr. Ramalho-Santos & team at LTRI! https://t.co/H36s7fcy0y
sinaihealth.ca
A critical transition in early human development is regulated not by our own genes, but by DNA elements called transposons that can move around the genome, Sinai Health researchers have found.
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@Dev_Cell Highlights: -Knockdown of LINE1 RNA induces reversion of naive hESCs to 8C-like cells -LINE1 RNA and PRC2 maintain nucleolar architecture in hESCs -Disruptions of the nucleolus de-repress the 8C program -Chr19 is enriched for 8C genes and dissociates from the nucleolus in 8CLCs
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📢@MRSantosLab - LINE1 RNA prevents developmental reversion of human embryonic stem cells to the 8 cell-like state! + new insights on PRC2, nucleolus and chr. 19. Revised and expanded now @Dev_Cell, free access until Dec 4th (earlier version on bioRxiv): https://t.co/tdVo5Gj2Xo
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Excellent team effort driven by postdoc Juan Zhang @LesleyZ618, working with Lamisa Mizan @mizanlam and Kirti Mittal in our lab, and in collaboration with Miguel Esteban, @jeffwranalab and @michaelhoffman. Thanks to all! Watch this space for an update in the next few months…
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The results point to a conserved role for LINE1 in repression of developmental reversion in both mouse (@MPercharde et al, Cell 2018) and human. The findings raise fascinating questions regarding transposon evolution and their role in genome compartmentalization.
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Our data indicate that LINE1 RNA promotes PRC2-mediated gene repression, in part via their role in maintenance of nucleolar architecture in naïve ESCs. Inhibition of PRC2 or direct disruption of the nucleolus leads to reactivation of the 8C program.
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Chromosome 19, where multiple key 8C genes are located, is enriched for genes upregulated upon LINE1 knockdown. Interestingly, Chromosome 19 is tightly associated with the nucleolus in naïve hESCs, but significantly less so in 8CLCs.
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LAD-seq, NAD-seq, DNA/RNA FISH reveal that LINE1 loci and RNA are enriched at the lamina and nucleolus. Key 8C genes like TPRX1 and H3.XY gain association with the lamina and nucleolus from 8CLCs to more developmentally advanced stages of hESCs, partially dependent on LINE1.
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Knockdown of human LINE1 RNA, via ASOs or CRISPRi, in naïve ESCs induces the 8-cell state, confirmed by bulk RNA-seq, single-cell RNA-seq, IF and dependence on TPRX1.
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📣Sharing the latest research from the @MRSantosLab, just released on bioRxiv! We found LINE1 RNA promotes nuclear compartmentalization to repress the 8-cell state in human embryonic stem cells. https://t.co/uE06Xx3QcY 👇Key findings:
biorxiv.org
The family of LINE1 transposable elements underwent a massive expansion in mammalian genomes. While traditionally viewed as a mutagenic selfish element, recent studies point to roles for LINE1 in...
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Recent review from @MRSantosLab. Everything you wanted to know about hypertranscription but were afraid to ask! This review discusses concepts of hypertranscription & technological advancements to probe global transcriptional shifts in stem cell biology. https://t.co/cvFS4AHWSY
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Postdoc positions in the @MRSantosLab! Please RT. Upcoming departure of several very successful lab members + new funding = postdoc positions! Exciting new avenues in mouse development, hypertranscription, dormancy and environment-epigenome interactions. https://t.co/1vyyptbUSb
mrsantoslab.org
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MRS reported latest from our lab on LINE1 in hESCs #ISSCR2024, Hamburg. Great to reconnect with many colleagues, + @bulutkarslioglu @EveCollignon. Excellent science, massive industry show. For respite: Art Museum, Caspar David Friedrich, “Wonderer above the sea of fog”, 1817.
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