Proud to share our two papers on mouse #prostate #organoids published in @emboreports and Cancer Letters @OncologyAdvance .THANKS to all collaborators and friends @ArmeniseHarvard @CIBIO_UniTrento

RT @CIBIO_UniTrento: 📢 A new work by @LunardiLabCIBIO is now out in @Biomol_MDPI. 🔬 Trpm8 Expression in Human and Mouse Castration Resistan….

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RT @ArmeniseHarvard: Proud to have supported @LunardiLabCIBIO and their important research - here's their last work on @biorxivpreprint abo….

Happy to share our work on prostate organoids! .GREAT THANKS to all our collaborators and to the @ArmeniseHarvard Foundation for the support!.

RT @MSCActions: What is the impact of food and nutrition on colorectal cancer? 🥬🍇 Researchers from the #MSCA TRIANGLE project investigate t….

RT @pezcoller: Delighted to announce the Winner of 2021 @pezcoller-@AACR International Award for Extraordinary Achievement in #Cancer #Rese….

RT @joseprubert: I'm honored and humbled to share the publication of TRIANGLE "Results in Brief" on @CORDIS_EU @MSCActions . .

Fig. 7 Intracellular calcium overload induced by pharmacological activation of TRPM8 could represent an interesting strategy to rise the efficacy of standard-of-care clinical protocols for the treatment of stage III/IV PCa characterized by high TRPM8 expression (10/10).

Fig. 6b Finally, concomitant administration of potent TRPM8 agonists with docetaxel or enzalutamide twists chemo and hormone therapy resistant lymph node metastatic LNCaP cells to treatments (9/10).

Fig. 6a Ex vivo experiments with the PCa PDX BM-18, which is characterized by a high IHC TRPM8 score, further demonstrate the efficacy of administering TRPM8 agonists to improve PCa cells response to X-ray treatments (8/10).

Fig.5 Annexin-V studies and WB analyses highlight the capacity of ERG-M40 to abate TRPM8 agonists-induced calcium cytotoxicity in TRPM8 overexpressing RWPE-1. Combination of TRPM8 agonists with X-rays is sufficient to induce cellular stress and a potent apoptotic response (7/10).

Fig. S6 Wild type and 6TM TRPM8 overexpressing RWPE1 have been engineered to express ERG(M40) alone or in combination with PTEN shRNAs. ERG(M40) confers migratory and invasive skills, while PTEN favors cell proliferation, loss of contact inhibition and growth in soft agar (6/10)

Fig. 4 Annexin-V quantification by FACS and western blot analysis of Caspase 3 and PARP cleavage identify a rapid and massive apoptotic induction in 6TM TRPM8 overexpressing RWPE-1 cells stimulated with TRPM8 agonists (5/10).

Fig. 3 Calcium imaging, macroscopic currents recording, and western blot analysis demonstrate inward calcium currents in 6TM TRPM8 overexpressing RWPE-1 stimulated with selective channel agonists (4/10).

Fig. 2 To model the different levels of TRPM8 expression in Stage III/IV PCa, RWPE-1 and LNCaP cells were KO for TRPM8 or manipulated to increase the amount of TRPM8 expression. Manipulation of TRPM8 levels does not impact cell morphology, growth or death. (3/10)

Fig.1 Expression datasets analyses and IHC studies show increased amount of 6TM TRPM8 in PCa compared to benign adjacent epithelial tissue. Of note, very high levels of TRPM8 characterize a large fraction of Stage III/IV tumors. (2/10)

First paper by Lunardi lab (and collaborators) is out in Nature Cell Death and Disease! This study led by @AlessandroAlai7 defines TRPM8 as an attractive molecular target for improving.standard-of-care treatments for locally advanced/high-risk PCa (1/10).

Very proud of our postDoc @joseprubert, starting soon his tenure track position at @WUR 🇳🇱 Ad maiora!.

Happy to share our work!.Congratulations to @AlessandroAlai7 and all authors!. @CIBIO_UniTrento.

RT @ArmeniseHarvard: 🦠🧫🧪🧬⚗️🦠🧫🧪🧬⚗️. ‼Aperte le candidature per il Career Development Award 2020‼️. 📅scadenza il 15 luglio 2020. 📝PER CANDI….