How does the 🧠 change between day and night? New work led by @sciencedame in @NatureComms finds diurnal changes in the choroid plexus and CSF including different translation, secretion, metabolism, and barrier properties. [1/9]

The choroid plexus is a circadian tissue and with Johnathan Lipton’s lab, we found it responds to external cues like steroids and feeding.[2/9]

Protein translation is downstream of the #circadian clock. In mouse choroid plexus, we find that more translation occurs at night. We collaborated with Myriam Heiman to identify differentially translated proteins between day and night [3/9]

TTR protein is more highly translated by the choroid plexus at night. TTR carries thyroid hormone. Levels of secreted CSF TTR were also higher at night. [4/9]

Using a ✨new✨TTR-mNEON 🟢 reporter mouse created by @Huixin_BJ in the lab and a wonderful collaboration with @pnkalugin @PaulSoden18 @andermann_mark , we observed CSF-TTR in real-time over multiple days. CSF-TTR even cycles when the lights stay out for days. [5/9]

The choroid plexus is the blood-CSF-barrier 🚪and we saw many barrier associated proteins change between day and night. We didn’t see changes in transcytosis, but apical tight junctions on choroid plexus epithelial cells were wider during the day. [7/9]

Collectively, these studies show key diurnal changes in the choroid plexus and generate a set of tools that can be used for future real-time study of CSF and choroid plexus. [8/9]

This type of work takes a team of experts. Sincere thanks to our additional collaborators @NeilCDani1 , @freshdipley , @aja_claire , Bradford, Suhasini, Josh, Morgan, The Lehtinen Lab, The Andermann lab, The @KanarekLab , The Heiman Lab, and The Lipton Lab on this work! 🥳[9/9]