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@kristamariephd@bsky.social Profile

@KristaTooTurnt

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Bacterial lipoprotein expert, cell envelope enthusiast, avid crocheter/crafter | Michigan Life Sciences Fellow πŸ‘©β€πŸ”¬ | UB, PSU, UM πŸ’™ she/her

Ann Arbor, MI
Joined December 2013
Don't wanna be here? Send us removal request.
Thrilled to share that my paper is officially published at PNAS!. So grateful to my collaborators, the ability to mentor students who became my co-authors, and to my mentor @nkoropatkin for her unending support!.
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Making the jump to the new app πŸ¦‹ follow me there for more science and lipoprotein alerts!.
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RT @UMMicroImmuno: ID & characterization of the lipoprotein N-acyltransferase in Bacteroides @nkoropatkin @KristaTo….
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Harvesting big, gloopy cell pellets of stinky gut bacteria
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Me in lab on a Friday when my multi-day protein purification fails
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RT @ASMicrobiology: This year's #AgarArt Contest theme is "What Brings You Joy?" ASM invites you to express your happiest moments through t….
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RT @prideinmicro: Pride in Microbiology is excited to announce our first virtual LGBTQ+ STEM Day Symposium!.Registration is open ~ abstract….
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Baby's first crosslinking experiment! πŸ”— Left is untreated, right is formaldehyde-treated. How does it look?
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πŸ“’πŸ“’πŸ“’ LIPOPROTEIN ALERT. First description of LolAB in Bacteroidetes! Both not essential (‼️) but big effects on gliding, morphology, protein localization, & more! Also not interchangeable with E. coli LolAB despite structural similarities 🀯. Bacteroidetes physiology is WILD.
@CCanimorsus
F_Renzi Lab #Capnocytophaga canimorsus
10 months
I am happy to share with you our last work on lipoprotein transport in Bacteroidetes!.
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Does anyone have experience with myc-tag purification of proteins, particularly w/ detergent? My myc-tagged lipoprotein is almost completely in the flowthrough, very little binding. Sample is in 0.5% DDM for solubilization. Too much? Does myc purification tolerate detergent? πŸ†˜πŸ™.
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RT @EvolvedBiofilm: Terrabacteria: redefining bacterial envelope diversity, biogenesis and evolution. @NatureRevMicro from @SGribaldo. http….
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My birthday was Tuesday and someone already made me say my age out loud πŸ₯΄πŸ« .
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This week I was confused why my Western didn't work. I later realized it was because I completely skipped adding my secondary. That was a first for me, it's never too late to make new mistakes!.
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2x the gel means double the fun, right? πŸ€ͺ
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Lipoprotein alert!!! Borrelia is wild.
@biorxiv_micrbio
bioRxiv Microbiology
11 months
Borrelia burgdorferi BB0346 is an Essential, Structurally Variant LolA Homolog that is Primarily Required for Homeostatic . #biorxiv_micrbio.
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Not trying to start any enzyme wars here butttttt. (template from @sketchscience)
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This single line on my Western has saved my project from sure doom
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RT @_KelvinKho: The bacterial cell wall is important for their physiology but what are its impacts on their interaction with the host? How….
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Reviewer comments are positive πŸ˜ƒ.
Not so patiently awaiting this email πŸ™πŸ€žπŸ™πŸ€ž
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Not so patiently awaiting this email πŸ™πŸ€žπŸ™πŸ€ž
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