Jonas Blomme
@JambenSloom
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Biologist | Postdoctoral Reseacher @UGent @VIBLifeSciences working on Seaweed | @PhycologyUgent and @GenomePlant lab | Tweets about plant and marine science
Gent, België
Joined February 2013
On top of all that, it was fun 🙂.
🔄#NGSB24 united academia & industry to explore the latest in #SyntheticBiology—from AI to living materials. Discover key insights, breakthroughs, & collaborations by reading the article here: .📖#synbio
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RT @McCormick_Lab: Our new review is out now – well done Ella, Sabina, Nicky and Charlotte – a great collaboration with the @mackinderlab!….
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RT @VIBConferences: Doing #synbio research & have not yet registered for #NGSB24? Don't miss out - sign up before tomorrow evening, when re….
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RT @SerenaRosignoli: 📣 A 20 months #postdoc position in Palmaria palmata #seaweed metabolism/physiology/genetics is now available in our gr….
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RT @BogaertKenny: Excited to share the Phaeoexplorer paper out today in Cell: Evolutionary genomics of the emergence of brown algae as key….
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RT @VIBConferences: This is your last chance to join the leading #syntheticbiology scientists from academia and industry at #NGSB24 in beau….
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Happy to hear your thoughts on our findings!.I want to thank @juliarraiza for the help with experiments and my supervisors @odclerck (@PhycologyUgent) and Thomas Jacobs (@PSB_VIB).
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The efficiency of vector-based CRISPR is low compared to RNPs in Ulva, but we show that co-editing of APT with a non-marker gene is feasible. We managed to install targeted mutations in three additional genes.Additional optimisations will be necessary for multiplex CRISPR.
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We evaluated vector-based CRISPR. We tested 31 vector configurations and found that bicistronic fusion of Cas9 to a resistance marker or incorporation of introns in Cas9 led to the most mutants. Curiously, vector DNA was often inserted at the target site.
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We observed that delivery of two RNPs resulted in the creation of large deletions in the APT marker gene. @juliarraiza helped to consolidate this observation and we could readily produce 20 kb deletions of Genomic DNA around the APT locus.
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Efficient CRISPR-based genome editing is difficult to achieve in many (macro)algae. After some initial tests, we benchmarked CRISPR editing of the marker gene APT using RNPs and a non-marker gene using targeted insertion of a resistance marker.
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We describe a chemical sensitivity screen for Ulva and its mutualistic bacteria. Ulva is sensitive to Blasticidin and we can confer resistance via Blasticidin deaminases. This nicely complements the current single selection systems to generate stable transformants in Ulva.
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Happy to share our new preprint ."Consolidating Ulva functional genomics: Gene editing and new selection systems".I highlight the main findings below.
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RT @KarasLab: 🚀 Breakthrough in #DiatomDNA delivery! Check out our newest preprint. Congrats to @ejlwalker @MarkPampuch Garvin Tran. Thank….
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RT @VIBConferences: 🧬TODAY is your last chance to submit your #SynBio abstract for the 5th edition of #NGSB24! Poster presenters can win th….
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RT @mackinderlab: A blueprint of the diatom pyrenoid reveals a Rubisco encapsulating protein Shell is critical for CO2 fixation. Is it esse….
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RT @VIBConferences: The #NGSB24 abstract deadline is next week!.The #synbio topics are:.⚙️Engineering synthetic molecules.⚙️Engineering pat….
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RT @SjonHartman: Thanks to the organizers for this wonderful #EPSR2024 in Paris! They're still looking for volunteers to organize the next….
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Happy to have kicked off #EPSR2024 with an overview of our tools and efforts to study Ulva biology! Excited to hear all the (PhD) talks today and tomorrow!.
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