Jake Montgomery
@JakeMont12
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Postdoc @uchicago trying to understand how weeds adapt and evolve with @jmKreiner. Views and spelling are my own.
Joined November 2013
This was a really fun project for me and it gave me an opportunity to work with some great folks at Bayer where they are trying to combat herbicide resistant weeds every day. If you made it this far, thanks for reading my first paper thread! 7/7
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This supports other experiments that suggest epyrifenicil will be able to control weeds that are resistant to some other PPO-inhibiting herbicides like lactofen or fomesafen. We found some new mutations, but these are tightly linked with G210 and are probably not important. 6/7
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I helped associate the herbicide damage phenotypes we collected with the variants detected in the PPO2 gene using a simple GWAS framework. The glycine deletion (G210) and another substitution (G399) affect efficacy, but R128 and V361 did not! 5/7
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By analyzing the gene sequences, we determined how many of them had mutations that we know cause resistance to other herbicides. Some mutations were pretty rare in our study, but we also did not intentionally sample resistant populations like other studies. 4/7
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From the same plants, we extracted RNA, made cDNA, and amplified the PPO2 gene using conserved primers. The sequencing team made barcoded illumina libraries out of the PCR product and sequenced, giving thousands of individual gene sequences. 3/7
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The team grew up plants from ~250 collections that were made during 2019. The dots are populations of Palmer amaranth, and the triangles are waterhemp. We sprayed 10 plants from each with epyrifenicil, a new PPO-inhibitor that completely controlled 92% of populations tested. 2/7
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Out this week in Weed Technology, a paper describing the project I helped with while I was an intern at @Bayer. Read the full story here https://t.co/BK2tXFaHD6. I usually don’t do these threads, but here goes nothing. 1/7
cambridge.org
Distribution of target site PPO-inhibiting herbicide resistance mutations in waterhemp and Palmer amaranth and association of epyrifenacil efficacy with PPX2 target site variants - Volume 39
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Latest work from the lab led by @yuhunghung in @ThePlantJournal! We engineered a RNA-binding protein to sort and protect a Cas9 transcript for long term expression! Can be applied to any engineered transcript as a UTR:
onlinelibrary.wiley.com
The low efficiency of transgene performance extends the long and expensive crop improvement pipeline, as well as hampers efforts to research the fundamental mechanisms of plant biology. We provide a...
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Following the firing of thousands of young USDA scientists yesterday, I will no longer be posting on this platform. You can find me on LinkedIn and Instagram.
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New preprint! We find a novel signal of positive selection in georeferenced genotype data, then use this signal to identify in-progress selective sweeps in the malaria vector Anopheles gambiae: https://t.co/lDYWTRmBy9
biorxiv.org
As organisms adapt to environmental changes, natural selection modifies the frequency of non-neutral alleles. For beneficial mutations, the outcome of this process may be a selective sweep, in which...
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Right after the WSSA GSO Student Luncheon, we’ve got an exciting Panel Discussion lined up! Hear firsthand insights from experts across industry, academia, and government—perfect for anyone looking to grow their network and gain fresh perspectives. Bring your questions! 🙌
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🚨 WSSA Student in-person Webinar! 🚨 Dive into Protein Modeling of Herbicide Resistance 🧬🌿 🗓️ Feb 25 ⏰ 4:45 💻 Bring your laptop & download pyMOL before the workshop! [ https://t.co/EZoyqDwYwm]
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CASTER: Direct species tree inference from whole-genome alignments. Super proud to have made small contributions to this incredibly clever work by the amazing Chao Zhang and his PhD advisor S. Mirarab. https://t.co/BkcrQuUKiU
science.org
Genomes contain mosaics of discordant evolutionary histories, challenging the accurate inference of the tree of life. Although genome-wide data are routinely used for discordance-aware phylogenomic...
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New episode is available! This week we have Dr. Todd Gaines on to talk about some novel genetic methods to control weeds. He covers the evolution of genomics and how it's helping scientists understand and combat herbicide resistance. Thanks for listening!
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The Slotkin lab is hiring for an exciting project that involves gene editing, transposable elements and plant transformation! Please RT!
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New preprint up! We sequenced hundreds of samples from across one of Earth's oldest living organisms - the Pando aspen clone - to understand how mutations accumulate and spread in long-lived clonal organisms. Our results were…surprising. 1/30
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Biggest medical discoveries of the week (🧵) 1/8 An engineered insulin, NNC2215, whose activity is adjusted by glucose concentration Its affinity for the insulin receptor drops >3-fold when glucose levels fall - reducing the risk of hypoglycaemia https://t.co/TJAx8vGN6R
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Many new machine learning tools for protein engineering have popped up in the last year. In these early days, it might be unclear how to actually use them for real molecular design tasks in industry. We demonstrate how to engineer a cholesterol drug and plastic degrading
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Curious about how major weed species are related? I published this phylogeny of ALS gene sequences from all the species currently hosted on WeedPedia along with the story of the international Weed Genomics Consortium. Check it out: https://t.co/AJQA7wV9nK
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Characterization of a novel eccDNA carrying both glyphosate and glufosinate target site due to co-duplication of their chromosomic native region - @theweednaldo @CuttiLuan @AimonePorri Ingo Meiners, Jens Lerchl, @GenomEric @NorsworthyJason
A Novel Genomic Rearrangement in the Amaranthus palmeri Extrachromosomal Circular DNA Provides Dual Herbicide Resistance to ... https://t.co/D74lOPhetY
#biorxiv_genomic
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