Jacob Bouchier
@JacobBouchier
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PhD from University of Missouri ➡️ Postdoc at Tufts Medical Center. Agrobacterium tumefaciens ➡️ Clostridioides difficile. He/Him/His. Tweets are my own.
Boston, MA
Joined August 2017
Out now in @PLOSGenetics! Activation of ChvG-ChvI regulon by cell wall stress confers resistance to β-lactam antibiotics and initiates surface spreading in Agrobacterium tumefaciens. It's great to see my first co-first author paper published!.
journals.plos.org
Author summary Soil dwelling bacteria reside in changing environments requiring them to frequently adapt to stressful conditions to ensure survival. The bacterial envelope provides structural...
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RT @ShenLabTufts: Happy holidays! Super fun making this fully edible gingerbread waterbath for our dept holiday party with this amazing gro….
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RT @shailabshrestha: Really happy to have this officially published! Thanks to @ShenLabTufts, @SGribaldo, and Najwa for their amazing suppo….
nature.com
Nature Communications - The enzymes FtsW and FtsI are thought to be essential for the synthesis of septal peptidoglycan (PG) during bacterial cell division. Here, Shrestha et al. show that the...
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RT @GlcNAcMurNAc: Analyses of cell wall synthesis in Clostridioides difficile reveal a diversification in cell division mechanisms in endos….
pubmed.ncbi.nlm.nih.gov
Current models of bacterial cell division assume that the core synthases of the multiprotein divisome complex, FtsW-FtsI, are the primary drivers of septal peptidoglycan (PG) synthesis. These enzymes...
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RT @DoerrLab: Postdoc positions open in Felipe Cava's lab! @CavaLab_MIMS Great lab to work in, and (confidently informed by recent personal….
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RT @ShamsCharania: After missing 51 straight games with a Grade 3 calf strain, Minnesota Timberwolves star Karl-Anthony Towns will return t….
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RT @Twolves_PR: NEWS: @theantedwards_ Named 2023 NBA All-Star . Edwards will be playing in his first NBA All-Star Game and he is the eighth….
nba.com
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RT @GlcNAcMurNAc: Peptidoglycan recycling mediated by an ABC transporter in the plant pathogen Agrobacterium tumefaciens .
pubmed.ncbi.nlm.nih.gov
During growth and division, the bacterial cell wall peptidoglycan (PG) is remodelled, resulting in the liberation of PG muropeptides which are typically reinternalized and recycled. Bacteria belong...
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Also, don't miss the companion paper on ChvGI in Caulobacter from @aaquinteroyanes and @HallezLab!
journals.plos.org
Author summary The cell envelope is the first barrier protecting cells from harsh environmental conditions, such as temperature, pH, oxidative and osmotic imbalances. It is also an obstacle for the...
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This was my first in-person meeting since the start of the pandemic. What a great group of scientists to learn from and network with!.
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RT @BrownlabMizzou: Congrats to @W_Melz! Her paper in @AppEnvMicro has been selected by the Editor in Chief @GemmaReguera of AEM for incl….
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RT @siegristpalmore: Better late than never. Big thanks to @caraboutte for the thoughtful highlight of @emily_melzer paper on cell wall dam….
journals.asm.org
The paper “Cell wall damage reveals spatial flexibility in peptidoglycan synthesis and a nonredundant role for RodA in mycobacteria” by Melzer et al. (E. S. Melzer, T. Kado, A. Garcia-Heredia, K. R....
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RT @plasmidsaurus: New dropbox has been set up at University of Missouri – Columbia @Mizzou! Pickups are every other Tuesday at 12pm, locat….
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Summary of our conclusions: ChvG/ChvI has a conserved role in sensing cell wall stress and conferring resistance to β-lactam antibiotics. Check out our preprint to see what happens if we deplete PBP1a even longer😉. Hint: ABC transporters. (10/10)
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This response appears to be conserved in Sinorhizobium meliloti and check out preprint of Alex out of the @HallezLab to see similar results in Caulobacter crescentus! (9/10).
biorxiv.org
Two-component systems (TCS) are often used by bacteria to rapidly assess and respond to environmental changes. The ChvG/ChvI (ChvGI) TCS conserved in α-proteobacteria is known for regulating expres...
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So does ChvG/ChvI respond to cell wall stress? We treated chvG and chvI deletion mutants with β-lactam antibiotics to find out. Turns out they were extremely sensitive to β-lactams, but not any other class of antibiotic we tested! (8/10).
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And finally, deletion of exoA, a gene required for the biosynthesis of succinoglycan, restored microcolony formation! (7/10)
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Next we looked at succinoglycan production. Succinoglycan is an exopolysaccharide secreted by Agrobacterium and other plant-associated members of the Rhizobiales. Succinoglycan pooling was observed in the PBP1a depletion using calcofluor staining. (6/10)
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So then what causes the spreading? We explored major operons that were differentially expressed in the depletion: flagellar motility, type VI secretion, and succinoglycan biosynthesis. We found that the spreading was independent of flagellar motility and type VI secretion! (5/10)
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