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Philip Gunkel Profile
Philip Gunkel

@GunkelPhilip

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@philipgunkel.bsky.social Postdoc @mpi_nat; Research focus: the nuclear pore complex and in particular the nuclear basket - composition and function.

Göttingen
Joined July 2020
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@GunkelPhilip
Philip Gunkel
6 months
See you on the other (hopefully brighter) side: @philipgunkel.bsky.social.
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@GunkelPhilip
Philip Gunkel
1 year
RT @embojournal: A nanobody toolkit for tracking or blocking nuclear pore complex (NPC) biogenesis identifies a checkpoint linking assembly….
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@GunkelPhilip
Philip Gunkel
2 years
How I sort GFP-tagged cells . .
@gunsnrosesgirl3
Science girl
2 years
Optical sorting technology that separates green and red tomatoes
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@GunkelPhilip
Philip Gunkel
2 years
RT @KarstenWeisLab: Congratulations to Janka Zsok and Elisa Dultz for their paper on the #nuclearpore basket in collaboration with @LucienW….
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@GunkelPhilip
Philip Gunkel
2 years
9/9 Finally, the ZC3HC1 structure predictions by AlphaFold2 illustrate an evolutionarily conserved NuBaID construction and enabled the redefinition of BLD boundaries. These predictions are consistent with the recent crystal structure of Pml39p by Hashimoto et al. @PalancadeLab.
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@GunkelPhilip
Philip Gunkel
2 years
8/9 We have further demonstrated that Pml39p enables interlinkage of Mlp1 polypeptides in nuclear foci in the absence of Nup60p. These results for the budding yeast protein Pml39 are consistent with features that we previously described for human ZC3HC1 (.
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@GunkelPhilip
Philip Gunkel
2 years
7/9 It was already known that Pml39p is a Mlp-interacting protein (Palancade et al. 2005 @DoyeValerie). We have now shown that Pml39p is required for positioning Mlp1p subpopulations at the NB, in line with a recent report by Bensidoun et al. @BensPierre4 @rnp_biology @zenklusd
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@GunkelPhilip
Philip Gunkel
2 years
6/9 We found DdZC3HC1 colocalizing with DdTPR at the nuclear envelope of D. discoideum Ax2 cells with an offset toward the nuclear interior relative to the labelling of FG-nucleoporins.
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@GunkelPhilip
Philip Gunkel
2 years
5/9 We further investigated the identified ZC3HC1 homologues of Dictyostelium discoideum (DdZC3HC1) and Saccharomyces cerevisiae (ScPml39p; already mentioned in Gunkel et al, 2021), which share the same characteristic and unique bimodular NuBaID signature.
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@GunkelPhilip
Philip Gunkel
2 years
4/9 We show that such a bimodular construction is evolutionarily conserved and allows the identification of a ZC3HC1 homologue in most taxa of the eukaryotic realm. Interestingly, no homologue can be identified in the famous model organism Drosophila.
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@GunkelPhilip
Philip Gunkel
2 years
3/9 Both domains (called BLD1 and BLD2) are required to establish a functional TPR-binding interface. These two BLDs are zinc finger domains that form an experimentally validated functional unit, which we have named NuBaID (nuclear basket interaction domain).
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@GunkelPhilip
Philip Gunkel
2 years
2/9 We describe the molecular prop­erties of ZC3HC1 that enable its initial binding to the nuclear basket (NB) and TPR. First, we mapped the minimal bimodular interaction domain of human ZC3HC1. Any further truncations resulted in loss of NB binding of such ZC3HC1 mutants.
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@GunkelPhilip
Philip Gunkel
2 years
RT @pleinerlab: Purifying eukaryotic proteins & their complexes at the scale & purity needed for structural, functional or mass spec charac….
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@GunkelPhilip
Philip Gunkel
3 years
It's that time of year again when you do flow cytometry and your brain goes like this:. ♪ Single cells, single cells,.… Oh! what fun it is to sort.single cells all day. ♪. @ghoshnotgosh #FACS #jinglebells #singlecells
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@GunkelPhilip
Philip Gunkel
3 years
RT @TinoPleiner: Cellular compartmentation requires stringent sorting of thousands of proteins to their correct destination. Our new pre-pr….
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@GunkelPhilip
Philip Gunkel
3 years
RT @StefanHellLabs: Interested in the raw data of our paper “MINSTED nanoscopy enters the Ångström localization range”? ..
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@GunkelPhilip
Philip Gunkel
3 years
Help the people (Russia, Iran, . ) to access free information:
@clausvonwagner
Claus von Wagner
3 years
Du fühlst Dich hilflos, aber möchtest was tun? Ein Vorschlag:. Teile anonym Deinen Internetzugang mit Menschen in Ländern, in denen das Internet gedrosselt/gesperrt ist, wie etwa im Iran. Wie das geht, findest Du hier: . #dieanstalt #iranrevolution2022
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@GunkelPhilip
Philip Gunkel
3 years
RT @Stefan_W_Hell: #STED at Å precision! See New article by Weber, von der Emde et al. Fantastic applications ahead….
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