I am very proud to announce my first first-author publication! With @enricoorsi and @PabloINik @LabNikel we explored the potential of the #Calvin cycle for biomanufacturing and concluded that it is indeed quite high!. Find the tweetorial (Xtorial?) here ⬇️.

RT @bioRxiv_synbio: CIFR (Clone-Integrate-Flip-out-Repeat): a toolset for iterative genome and pathway engineering of Gram-negative bacteri….

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RT @bioRxiv_synbio: Metabolic engineering of yeast for de novo production of kratom monoterpene indole alkaloids . .

RT @enricoorsi: 🌱C1 feedstocks (formate, methanol, etc.) are transforming #biomanufacturing. By using noncanonical redox cofactors (NCRCs)….

RT @LabNikel: New publication!📰.Check out how we engineered #Pseudomonas putida as a whole-cell #biosensor for a variety of chemicals!. Gre….

Brava @FavoinoGiusi 🥰🦠🏮.

(12/11) (ops) At the beginning of September I had the honor and pleasure to present this work at #Pseudomonas2024 in Copenhagen 🏙️ (the place I call home nowadays). It was really great to get inputs from the Pseudomonas community! Thanks for the opportunity @MicrobioSoc 🙏

@PabloINik @LabNikel @DTUBiosustain 🧰 The CIFR toolbox will soon be available online, but feel free to reach out directly to get the plasmids from us! 📨 We’d love feedback or questions from the SynBio community on CIFR! 🔍🧬(11/11).

@PabloINik 🙏 Special thanks to Clara and Melina, former @LabNikel students, for constructing the plasmid toolbox, and the @DTUBiosustain analytics team for HPLC setup help! 👏🔬 (10/11).

👥 This work was led by Francesco Luppino, @PabloINik and me (@FilFederici) – big thanks to them for their dedication! This pre-print builds on Francesco’s master thesis, which involved an incredible amount of lab work. Cheers to you, Fra! 🎉👏 (9/11).

🥕 We then tackled pathway balancing for carotenoid production. Using CIFR, we achieved high zeaxanthin content and full conversion of intermediates to final products! 💪🍊 (8/11)

🎨 We applied CIFR to integrate a palette of chromoproteins in P. putida, both individually and in combinations. Look at our super colorful plates! 🌈✨ (7/11)

🔄 With no antibiotic resistance cassettes and the shift from attP/attB to attR/attL during excision, this workflow is iterative! 🚀 Want to increase copy number? 📈 Or stack additional pathways? CIFR makes it possible! 💡 (6/11)

⚙️ pFNC worked in all three bugs, too! Efficiency was very high in P. putida and C. necator, but lower in E. coli. Make sure to test efficiency before starting to engineer your favorite bacterium with CIFR! 🧫🧪 (5/11)

🧹 A second transformation with pFNC plasmid removes the antibiotic resistance cassette from your chosen strains, leaving your engineered bugs antibiotic-free! 🦠🔄 (4/11)

🔬 We tested CIFR by integrating msfGFP into Escherichia coli, Pseudomonas putida, and Cupriavidus necator – and it worked in all of them, falling in a different place every time! 🎉 In P. putida, integration in different loci yielded varied expression levels! (3/11)

We created two plasmid sets: pCIFR and pFNC. pCIFR, derived from pBAMD1 (, enables transposition of modules into the target bacteria’s genome. Pick your favorite integrant by phenotype! 🎨 Is it colored? ✨ Fluorescent? Or maybe growth-coupled? 🌱 (2/11)

📢 Happy to announce that the first experimental pre-print of my PhD is out! We (@LabNikel) present CIFR (Clone, Integrate, Flip-Out, Repeat): a transposon-based tool for integrating synthetic modules in Gram-negative bacteria (! A X-torial 👇 (1/11)

RT @SebWenk: 🚨 Job alert 🚨 Please retweet 🚨

RT @PabloINik: Part of the @LabNikel gang at the #Pseudomonas2024 opening! Check the work (posters & talks) by @OscarPuiggene, @mgracialvan….

RT @PlanasFerran: @barometrecast avui els @XiquetsCPH hem fet una actuació amb pd4 d’entrada, 3d6, 4d6a, id5d6, 5d6 i pd4 pel nostre desé a….