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Jonathan Bouvette Profile
Jonathan Bouvette

@CryoJB

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304
Following
309
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24
Statuses
285

Cryo-EM core NIEHS. Opinions are my own.

Durham, NC
Joined September 2014
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@CryoJB
Jonathan Bouvette
2 years
Met amazing people at my first #3DEM @GRC this week! . For those who missed #3DEM, I am pleased to announce that I will be joining @Princeton @PrincetonMolBio in just 2 months as a core manager to lead the cryoEM efforts. Stay tuned for great science to come! #CryoEM #Teamtomo.
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@CryoJB
Jonathan Bouvette
2 years
Please check out @scipion_em smartscope plugin to feedback your preprocessing data to SmartScope. Now in Beta!.
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github.com
Plugin to manage the interaction with the SmartScope framework. - scipion-em/scipion-em-smartscope
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@CryoJB
Jonathan Bouvette
2 years
#CryoEM SmartScope v0.9.2 is out!.Here are a few upgrades:.- Aperture Control w/ SerialEM 4.1-beta @Nexperion .- Great progress on CryoARM support. - More flexibility about data storage locations. - Easier installation procedure. Full notes at:
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@CryoJB
Jonathan Bouvette
2 years
I received a broken litre of hot sauce from @Walmart and other items that got ruined during shipping . No offer to reship nor honor the prices at the time of purchase . They offered a refund- that's it. #doneWithWalmart.#CustomerExperience.Wouldn't have happened @Target
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@CryoJB
Jonathan Bouvette
2 years
From having first-hand experience with this software, it is incredible. The tomo part particularly, fills a gap in the field. Never build a tomogram manually again. And for SPA preprocessing, you can't get faster than this with parallelization up to your cluster limits.
@naturemethods
Nature Methods
2 years
Out today from the Bartesaghi lab! nextPYP is a turn-key framework for single-particle cryo-ET that streamlines complex data analysis pipelines, from pre-processing of tilt series to high-resolution refinement, for efficient analysis of large datasets.
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@CryoJB
Jonathan Bouvette
2 years
RT @naturemethods: Out today from the Bartesaghi lab! nextPYP is a turn-key framework for single-particle cryo-ET that streamlines complex….
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@CryoJB
Jonathan Bouvette
2 years
When you're at lunch and there's an atlas of the table on your leg. #SeeingCryoEMeverywhere 😅 #CryoEM
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@CryoJB
Jonathan Bouvette
2 years
RT @NinaSteele17: happened IRL.Me: I’m a breastfeeding mom so carrying milk/ice packs frozen solid.Tsa: where is your baby.Me: not with me…….
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@CryoJB
Jonathan Bouvette
2 years
RT @GroupZhang: Great work by Anna-Sophia Krebs in collaboration with Juan Perilla and Alberto Bartesaghi's labs, demonstrating the molec….
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biorxiv.org
A significant part of the human genome consists of endogenous retroviruses sequences. Human endogenous retrovirus K (HERV-K) is the most recently acquired endogenous retrovirus, is activated and...
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@CryoJB
Jonathan Bouvette
2 years
RT @Gagamboy18: Our paper is out! Congratulations to @Cassandra_Hayne @TheHayneLab @RobinStanleyLab at @NIEHS ! I had fun image processing….
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@CryoJB
Jonathan Bouvette
2 years
I was considering switching my @djangoproject to a @JavaScript frontend until I discovered @htmx_org! I very much love to focus on writing python and have everything in one place.
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@CryoJB
Jonathan Bouvette
2 years
SmartScope v0.9 collecting at a rate of 421 movies/h on R2/1, 7-shots per hole, very conservative 4.5um max beam-image shift. Setup time after the scope was aligned: 20 min. Fully automated from there. 😅Just need to improve the display of the targets at the medium mag now.
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@CryoJB
Jonathan Bouvette
2 years
Smartscope Documentation now has its own repository
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@CryoJB
Jonathan Bouvette
2 years
Simply provide a few information and SmartScope will suggest optimized multishot per hole pattern with some stats about total hole coverage and fraction of the images that is within the hole.
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@CryoJB
Jonathan Bouvette
2 years
SmartScope v0.9 is out! . Highlights of the release are (details in thread):.- Multishot per hole.- Automatic focus position from hole lattice orientation and spacing. - Redesigned docs website. Join our Google group! (
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@CryoJB
Jonathan Bouvette
2 years
RT @wsleal2014: My in-person @ucdavis biochem class was "ZOOM-bombed" today by a structural biologist @GntlmnScientist from @UCBUSA. We mad….
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@CryoJB
Jonathan Bouvette
2 years
SmartScope now has a forum for discussion. Please join! Oh . and also multi-shot per hole with automated pattern is in beta.
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@CryoJB
Jonathan Bouvette
2 years
Today I tried the Light ‘em up to set collection on a grid where i knew there were a lot of empty holes. It worked nicely! Look at all the crappy data I can skip! Thanks @WimJHH for the tool.
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@CryoJB
Jonathan Bouvette
3 years
RT @AmandaRiccioPhD: Almost 3 years later and 2.5 years with a child in daycare. Only took 1 mitochondria @GordonConf with no one masked (t….
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