A novel catalytic mode of aldoxime dehydratases for the abiotic radical ring-opening reaction of cyclic ketoximes is reported. Aldoxime dehydratase from Nocardioides simplex was found to efficiently...

Polyion complex micelles composed of blends of ABA and AB block copolymers can form coacervates with a hexagonally packed cylinder nanostructure after heating. The resulting coacervates can release...

We have engineered the heme biosynthetic pathway in E. coli to develop an efficient cofactor supplementation system for hemoproteins. Through the incorporation of the heterogeneous C4 pathway, the...

Nature Communications - Modular polyketide synthases (PKSs) are multi-domain enzymes responsible for the biosynthesis of polyketide antibiotics. Here, authors show the potential of ancestral...

SLIPT-PM enables the translocation of tag-fused proteins from the cytoplasm to the plasma membrane using a synthetic self-localizing ligand. This user-friendly system permits reversible control of...

T7 RNA polymerase (RNAP) is a highly active single-peptide polymerase that is widely used for in vitro RNA synthesis. T7 RNAP synthesizes RNA with a heterogeneous 3′-end, which sometimes causes...

Protein redesign is an enzyme engineering strategy that enhances a template enzyme’s properties by introducing mutations. Although the method is broadly employed for enzyme design, finding optimal...

Single-molecule DNA computing, integrated with nanopore technology, enables real-time molecular computation. The single-molecule logic unit (sMOLU) system, using α-hemolysin nanopores and an avidin...

Cross-coupling reactions between short-lifetime radicals are challenging reactions in organic chemistry. Here, we report the development of an N-heterocyclic carbene (NHC)-mediated radical coupling...