Alushin_Lab
@Alushin_Lab
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Interested in cryoEM, cytoskeleton, and mechanosensation at the molecular level.
New York, USA
Joined October 2017
How do cells move, divide, and sense their surroundings? The answer lies in the cytoskeleton--and when it malfunctions, cancer and birth defects follow. @Alushin_Lab at @RockefellerUniv has developed new tools to probe this tiny, dynamic cell scaffolding. https://t.co/NlBcWZQxN0
rockefeller.edu
Inside each of your cells, there’s a microscopic scaffolding that helps determine what the cell looks like, how it moves, and how it responds to its surroundings. This internal structure, called the...
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https://t.co/bTd7QeyQlG The latest research article from the Ti Lab is finally out today in Nature Physics.
nature.com
Nature Physics - Proteins interact with both the exterior and interior of microtubules. Here the relationship between microtubule building blocks and the accessibility of the microtubule interior...
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Applications for faculty positions at Rockefeller are now open! We are seeking exceptional, creative scientists to join our faculty. Learn more below: https://t.co/EVtKiEgNBf
rockefeller.edu
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Vought has ordered a halt on all extramural NIH funding for the rest of the FY. No words. You cannot let this stand @SenateDems @SenateGOP @NIHDirector_Jay @hubermanlab
https://t.co/tJSaWl7Ddk Trump Administration Puts New Chokehold on Billions in Health-Research
wsj.com
The administration halted and then restarted billions in new research grants flowing from the National Institutes of Health.
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Keith Hamilton from the @Alushin_Lab at Rockefeller University gave a deep dive into the first step of any structural biological workflow: sample preparation. Through test cases he showed the balance of what is easily doable versus preserving biology. https://t.co/oVFTltoBYd
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New study in @ScienceAdvances: the Alushin lab shows how afadin strengthens cytoskeletal engagement by the cadherin-catenin cell adhesion complex. Dysregulated afadin expression has been associated with carcinogenesis and cancer metastasis. https://t.co/GipzsP7JRr
science.org
Afadin strengthens adherens junctions by stabilizing cadherin-catenin complex clusters on curved F-actin.
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https://t.co/BBiG2N9Tkr Just published! We uncovered a molecular machine that connects the cell's two major structural networks revealing how cells accomplish precise internal reorganization - a process disrupted in diseases ranging from cancer to multiple sclerosis.
science.org
Nesprin-2 Giant links the nucleus to the cytoskeleton, enabling actin transport and nuclear movement through kinesin-1 activation.
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Congrats to Rui, as well as co-authors @mattabolic_rate and @XiaoyuSun_LIM. A relink to our original preprint is below.
biorxiv.org
The E-cadherin–β-catenin–αE-catenin (cadherin-catenin) complex couples the cytoskeletons of neighboring cells at adherens junctions (AJs) to mediate force transmission across epithelia. Mechanical...
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The first author, Rui Gong, is opening his lab at the Shenzhen University of Advanced Science and Technology as a newly minted assistant professor. Anyone interested in structural biology / cytoskeleton research opportunities in China should reach out!
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We thank the reviewers for their supportive comments that improved the text, as well as the journal for fast handling of our paper.
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This mediates the formation of cadherin-catenin complex clusters along actin filament strands, which are associated with filament curvature. It will be interesting to see if similar assemblies form in cell-cell adhesions where forces are large, e.g. tricellular junctions.
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A couple key take homes: A segment of afadin’s coiled-coil domain bridges adjacent α-catenin actin binding domains along actin filaments, binding and stabilizing flexible parts of α-catenin implicated in mechanical regulation of adhesion.
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Some happy news. Pleased to see our paper showing how afadin strengthens cytoskeletal engagement by the cadherin-catenin cell adhesion complex out in @ScienceAdvances :
science.org
Afadin strengthens adherens junctions by stabilizing cadherin-catenin complex clusters on curved F-actin.
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Finally, here is a relink to our original preprint. Congrats to first author Donovan Phua and co-author @XiaoyuSun_LIM !
biorxiv.org
As the cytoskeleton sustains cell and tissue forces, it incurs physical damage that must be repaired to maintain mechanical homeostasis. The LIM-domain protein zyxin detects force-induced ruptures in...
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Also, this work was completely funded by the National Institutes of Health (NIGMS, and an NHLBI fellowship to first author Donovan Phua). Federal support makes fundamental science like this possible, a foundation for the next generation of therapies to improve human health.
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We'd like to thank @CurrentBiology for a very smooth (and fast!) publishing experience. 11/10 would recommend.
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After revision in response to very constructive reviewer comments, the paper features additional evidence that zyxin and other LIM proteins form force-dependent assemblies which lack underlying F-actin. Figuring out how these come together is the next frontier.
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Jazzed to join the cool kids club and have our paper reporting the reconstitution of zyxin-mediated repair of contractile actin networks appear in @CurrentBiology !
cell.com
Phua et al. report that LIM proteins form force-dependent assemblies that bridge broken filament fragments. Zyxin assemblies coordinate F-actin nucleation by VASP and its crosslinking into aligned...
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Thrilled to share our work with the Ben Black Lab, led by a co-mentored star MD/PhD student Katie Kixmoeller (@PennMedicine,@BMBGGUPENN,@BB_UPenn), now in @CellCellPress! We used cryo-ET to study kinetochore features in human mitotic chromosomes. 🧬🔬❄️ https://t.co/4yR1CrGybM
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