Alexandra Hochstetler, PhD
@Alex_H_Bio
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Towards biofidelic models 🐷 | https://t.co/fyeHSD3Qcg Choroid Plexus Biology 🔬 | Physiologist
Boston, MA
Joined January 2022
RT @LehtinenLab: 🌟Check out @HydroAssoc white paper on research priorities for hydrocephalus from current (@Alex_H_Bio and @chehnly1) and p….
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RT @costinebartell: 6. Your child or grandchild or niece or nephew might get worse care if they ever end up in the ICU with a brain injury….
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RT @costinebartell: 5. The grant is studying if we should stop seizures in children in the PICU and the method that is the safest. This wor….
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RT @costinebartell: I've been encouraged to share what is happening with the Trump administration and how it affects my research: 1. my gra….
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Sometimes I get to go out to the middle of nowhere in pursuit of samples and it can be really pretty 🐷💧🧠
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Happy to be a part of this massive effort led by Peter to expand our toolkit for studying CSF in awake, behaving animals! 🎉.
I’m thrilled to share our preprint presenting MORSE: a new way to simultaneously and quantitatively track patterns of neuromodulators/neuropeptides in vitro & in vivo using spatially multiplexed 3D imaging of 10+ green optical sensors on a microendoscope.
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RT @ScienceYael: 11/ Huge thanks to my co-authors @Alex_H_Bio and @LehtinenLab. The ChP is small but mighty, and we hope this review inspi….
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RT @ScienceYael: 🧵1/ 🚨 TL cleanse good news! 🚨 Excited to share our review, Choroid Plexus Pathophysiology, now out in Annual Review of Pat….
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I've never felt more like an elder millennial than when trying to post a thread on this app I never post on and accidentally doing it wrong after I already tagged a bunch of people 🤡 🤣.
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@ScienceYael, Peace Oloko, Benjamin Baskin, Andrew Ding-Su, @tawnystinson , Declan McGuone, Robin Haynes, @LehtinenLab & collaborators @csadegh , @ScienceDame, @lab_paredes, Misty Williams-Fritze, Corinna Beale, our undergraduate interns, & others!.@biorxiv_neurosci @MGH_RI.
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11/ Thanks to the team, our collaborators & funding sources, and all supporting pediatric brain injury research. Feedback & discussions welcome! 📩.
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10/ Big picture: Our study highlights how pediatric TBI affects ion transport, linking chloride dysregulation to injury severity and seizures. By modeling these processes in swine, we hope to bridge gaps in TBI research. 🧩.
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10/ Therapeutic implications? Targeting NKCC1 or SPAK might mitigate injury progression in infants, while interventions like MMP inhibition (PMID: 38190820) could address damage in toddlers. Age-specific therapies could be key!.
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9/ Human autopsy data corroborated our findings, showing region-specific alterations in pNKCC1 after pediatric TBI, particularly in damaged white matter & the ChP. The parallels between swine & human data are striking.
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8/ TBI also disrupted chloride cotransporters in the choroid plexus, with pNKCC1 signal becoming dysregulated. This suggests ionic homeostasis at brain-fluid interfaces is critical post-injury.
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7/ We next employed IHC for neuronal pNKCC1, and found it significantly increased in infant piglets with TBI, and across both ages, amount of neuronal pNKCC1 positively correlated with seizure duration and injury severity.
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6/ Interestingly, TBI affected the cortex & hippocampus differently. In our model, the cortex exhibits structural damage, but the hippocampus is spared. Yet, in both regions, infant piglets with TBI showed increased NKCC1, KCC2, and SPAK compared to toddlers.
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5/ Key finding: In swine, the GABA switch occurs during infancy across cortex and hippocampus, aligning with data in humans. This makes swine an excellent translational model for pediatric brain injury research.
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4/ Why chloride cotransporters? The GABA "switch"—from excitatory to inhibitory signaling—depends on NKCC1/KCC2 balance. We explored this hypothesis across piglet neurodevelopment.
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