David Edgell
@megatev
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Genome editing, synthetic biology and mobile genetic elements.
Western Univ Biochemistry
Joined June 2016
All the details in the paper! Another stellar collaboration with #GloorLab. Supported by @CIHR_IRSC @westernuBiochem @SchulichMedDent.
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crisprHAL ML model found poor activity at PAM sites with GATC sequences. seem familiar? GATC is DNA adenine methyltransferase site in bacteria. Large scale in vivo assays, Nanopore sequencing, and in vitro assays all show that G*ATC methylation impacts SaCas9 activity.
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Paper 🚨📢! Adenine methylation in PAM sequence regulates Staph aureus Cas9 activity in bacteria. Superstar collab between #DaltonHam @nortnlentivirus.
biorxiv.org
Cas9 nucleases are the effectors of the class 2 type II CRISPR system in bacteria and function to restrict invading DNA. They can also be used with single guide RNAs (sgRNAs) as antimicrobials and...
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Amazing work by @gary_wfoo with big assist from @deheinrichs. Supported by @CIHR_IRSC @westernuBiochem @SchulichMedDent.
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New paper 📢. What happens when @gary_wfoo genetically entangles a thermally-regulated meganuclease and colE9 immunity protein? Ultra-stable biocontainment in the mammalian gut!!
biorxiv.org
Imbalances in the mammalian gut are associated with acute and chronic conditions, and using engineered probiotic strains to deliver synthetic constructs to treat them is a promising strategy....
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Incredibly lucky to have played a small role in this amazing paper. Congrats to all involved!! @SchildPoulter @SchulichMedDent @WesternU #Zhalaboratory @Columbia.
nature.com
Nature - Studies on the essentiality of Ku in human cells reveal that Ku interacts with diverse double-stranded RNA molecules, including antisense Alu, and enables tolerance of Alu sequence...
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Congrats to trainees @THamilton26 @brjoris @nortnlentivirus and collaborators @BogumilKaras @gbgloor @RodrigueLab for their paper being selected as one of the Top 25 ACS papers for World Micrbiome Day! .
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RT @CanJMicrobio: 📣 @CanJMicrobio is accepting papers for a collection showcasing Canadian research in #Synthetic_Biology. 🧬More info: htt….
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RT @SynBioCanada: Excited to spread the word about @CanJMicrobio call for papers! Advances in Synthetic Biology in Canada 🍁🧬👩🔬👨🔬 https://….
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5/n Early stages yet but excited about potential for rep-editing. Stay tuned for updates.
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4/n Guess what? Size does matter - everything fits within a single AAV vector (TevSaCas9 + repair guide). NO need for co-delivery of exogenous repair factors, accessory repair proteins/templates, or small/molecules/proteins to modify repair pathways.
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3/n Nucleotide substitutions, deletions and insertions all possible with high efficiency and fidelity. Some clever molecular biology to ensure expression of nuclease editor and repair guide RNA from polymerase II transcript.
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2/n Huge shoutout to #NhanHuynh for spearheading development of RNA-templated repair (rep-editing). Our editing platform uses a small gene editor (TevSaCas9). Endogenous repair factors (Pol theta, Rad52) are recruited to repair DNA breaks using an RNA repair template guide RNA.
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📢New gene editing technology This is result of amazing collaboration with @SpecificBio and supported by @NSERC_CRSNG @MitacsCanada @LumiraVentures @adMare_Bio @westernuBiochem @SchulichMedDent 🧵👇.
biorxiv.org
Recent studies have revealed a role for RNA in the repair of DNA double-strand breaks. Here, we show that the asymmetric DNA overhangs generated by the small TevSaCas9 dual nuclease informs a simple...
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RT @SchulichMedDent: Projects by #SchulichMedDent researchers have received a combined $2 million in project grant funding from @CIHR_IRSC.….
schulich.uwo.ca
CIHR project grants will help Schulich Medicine & Dentistry researchers, like David Edgell and Dean Betts, create news answers to our health challenges.
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RT @CSM_SCM2024: @CSM_SCM When attending @CSM_SCM2024, be sure to catch our Symposia on Synthetic Biology, and Antimicrobial Resistance. Co….
cdnsciencepub.com
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RT @SynBioCanada: Canadian graduate students and postdocs have gone without a raise in 20 years! @cafreeland they need help more than ever,….
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RT @McGavLab: Please follow @CSM_SCM2024 and visit our conference Web Site to view our exciting speaker lineup and….
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RT @cdnsciencepub: 📣 Call For Papers: The Canadian Journal of Microbiology invites submissions on #synbio, #CRISPR, #GeneticEngineering, #B….
cdnsciencepub.com
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📢 #synthetic_biologists!!! Drawing your attention to special issue of Canadian Journal of Microbiology devoted to synthetic biology. Submissions now be accepted - research, commentaries, reviews. Spread the word to your colleagues!
cdnsciencepub.com
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