Many thanks to all collaborators, @JulieKlepstad, @EdoaurdHannezo, @prlunde, Ala Trusina, and to Elke Ober for her supervision.

Together, our work shows that heterogeneous progenitor contributions to tissue architecture-defining cell type proportions and postembryonic organ growth are key mechanisms forming the adult liver.

Finally, we characterized liver morphology during growth and revealed distinct ventral lobe formation.

Interestingly, we observed a transient appearance of polyploid cells during a massive postembryonic growth phase.

Extending lineage tracing to adulthood determined that embryonic cells undergo spatially heterogeneous three-dimensional growth linked to distinct environments. Strikingly, giant clusters comprising almost half a ventral lobe suggest lobe-specific dominant-like growth behaviors.

.… also bipotent hepatoblasts, with the majority of hepatoblasts only contributing to the hepatocyte lineage.

Moving to the in vivo system we probed this prediction with lineage tracing experiments using the multispectral FRaeppli system and identified not only unipotent hepatoblasts, but….

1. Mathematical modeling predicts that a heterogeneous progenitor lineage potential is sufficient to achieve 1:9 BEC-to-hepatocyte ratio proportions.

1. First, we analyze cell type proportions of differentiated hepatocytes and biliary epithelial cells (BECs) in vivo. We show that a precise 1:9 BEC-to-hepatocyte ratio is established fast. But how is such a ratio established?

How do liver progenitors contribute to the functional organ? Happy to share this work, in which we used lineage tracing in the zebrafish 🐟 liver to study the contribution of individual liver progenitors to the different cell lineages and postembryonic organ growth🧵👇 (1/10).

RT @DBioimaging: Python for BioImage Analysis workshop! Registration open! .🔴 Sign up for this 2-days workshop orga….

RT @Paloma_IMG: And that's a wrap! The zebrafish team had so much fun teaching the #embryo23 course at @MBLScience last week! @LabPiotrowsk….

RT @focalplane_jcs: At #celldyn2023, @iris_unterweger described their use of FRaeppli, a multispectral imaging toolbox, to determine hepato….

We are excited to share the newest preprint from our team! .LiverZap: A chemoptogenetic tool for global and locally restricted hepatocyte ablation to study cellular behaviours in liver regeneration

I am very excited to share the preprint I worked on during my PhD in the group of Elke Ober with great collaborators @JulieKlepstad, @EdouardHannezo, Pia Lundegaard and Ala Trusina!.