Domenico Modaffari
@d_modaffari
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PhD Student at the University of Edinburgh
Joined June 2022
All plasmids available on Addgene 🧫., and a detailed step-by-step protocol on �
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When testing fluorescent tags, we found the new mStayGold to be much more photostable but significantly dimmer than mNeonGreen in our system 📸 [3/4]
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Using this system, we routinely perform markerless tagging of proteins at their endogenous loci in Aspergillus nidulans. We've created Aspergillus-optimized versions of several recent fluorescent proteins to support this work 🔬 [2/4]
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Happy to share a side project of my PhD work! We've simplified a popular CRISPR system for filamentous fungi. Create your sgRNA plasmid in a single-step Golden Gate reaction between a vector and 2 annealed oligos 🧬 [1/4]
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RT @EdBiophysics: LIVE-PAINT imaging of actin in filamentous fungi Aspergillus nidulans by @haresh_bhaskar_ and @d_modaffari on our @ONI mi….
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