Bryson Lab
@TheBrysonLab
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Engineering innate immunity // Host-pathogen interactions // Systems Biology // Tuberculosis @MIT @MITDeptOfBE
Boston, MA
Joined September 2017
Does anyone have an in vitro T cell priming protocol they have successfully used in human PBMCs?
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AbMAP, our work on adapting PLMs for antibodies, is now out in @PNASNews. A veritable zoo of PLMs now exists, so why should you care? Well, AbMAP reflects an opinionated design philosophy about antibody representation. If that design fits your need, it may be great for you! 1/
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Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?
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Please get in touch if this would be of interest to you! We are happy to host collaborators in our lab to learn how we do our MS analyses
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We can also ask if peptides that you are excited about are presented by Mtb-infected cells.
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We would love to collaborate with groups with HLA typed samples where we can interrogate the immunogenicity of these peptides.
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We have the capacity to buy HLA typed blood and perform high sensitivity MS analysis to define Mtb-derived peptides on MHC-I and MHC-II.
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TB friends! We are excited to expand our studies of MHC presentation of Mtb peptides to more alleles that capture human diversity. If you are involved in any ongoing human cohort studies where you are interested in studying T cell responses, we would love to collaborate!
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Does anyone have favorite cell membrane/cytoplasm dyes for multi-day live cell imaging that are ideally 405-excitable and don’t induce appreciable phototoxicity?
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There is still about a month to apply to our faculty position in #immunology and #immunoengineering
@MITdeptofBE! We look forward to reading your applications
Join us @MITdeptofBE! We are searching for a tenure-track faculty colleague at the interface of #immunology and #biologicalengineering. Happy to answer questions about the BE department and junior faculty life at MIT (both great). Please share widely! https://t.co/xOFpH7bsKa
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Anyone else see this paper? Perhaps a genome architecture explanation for NOS2 in mice v humans. https://t.co/7xMEIExzfe
cell.com
It is currently unclear how evolutionary genome rearrangements affecting the mouse and human genomes influence the expression of genes important in immunity. Gilbertson et al. report that evolution...
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TB friends! Anyone have a human CD4+ T cell clone that recognizes PPE18/Rv1196?
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This approach has been truly indispensable for us. Rigorous biochemical validation of immunopeptidomics data is super important!
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Anyone know where I can order milligram quantities of human MHC-II (with or without peptide?) NIH tetramer core is great but only does hundreds of micrograms.
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Has anyone had success with targeting proteins to the lysosome using something other than LAMP sequences?
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#TB friends!! Deadlines are approaching for @KeystoneSymp #Tuberculosis : Heterogeneity from Models to Human Disease, this February in Boston! See scholarship, abstract and discount reg dates: https://t.co/3iZ9dbA8eG
#KSTB25
keystonesymposia.org
Join us at the Keystone Symposia on Tuberculosis: Heterogeneity from Experimental Models to Human Disease, February 2025, in Boston, with field leaders!
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A big congrats to @OwenKLeddy for this tour de force and a huge thank you to our amazing collaborators @Qingyun_Liu and @JaTcells. OK I'm drinking champagne now -- bye!
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(3) If I were a betting man, I think you know what class of Mtb antigens, I’d be betting on for T cell vaccines.
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(2) We need help exploring more human HLA diversity – if we don’t do this now, new TB subunit vaccines may have trouble generalizing across the global population. Particularly, we would love better data on MHC-II allele diversity geographically.
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(1) We believe that there needs to be continued investment in new approaches to do cool stuff in human immune cells especially in the context of TB (gene delivery, immunologic characterization, etc). Prioritizing human observations to guide TB vaccine development is essential.
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