And importantly -- thank you to Owen, Forest White and our many amazing collaborators! Please get in touch if you want to chat more or have questions! And watch this space for the next two chapters of the Mtb-MHC saga (coming soon, they are very very exciting)

We're excited! These are needle in the haystack measurements out of the BL3 that Owen executed beautifully. These studies also provide us a path to antigen discovery and vaccine optimization in human cells. And it's generalizable -- we can definitely extend to other pathogens!

TL, DR: (1) Type VII secretion system substrates strike again -- enriched for presentation on MHC-II. (2) Lysosomal targeting enhances presentation on MHC-II by orders of magnitude. (3) Coexpression of heterodimeric pairs can enhance presentation of Mtb-derived peptides.

Here, we apply immunopeptidomics in primary human phagocytes to identify Mtb peptides presented by infected cells on MHC-II and then optimize mRNA vaccines encoding these antigens.

Excited to share our newest manuscript on antigen discovery and vaccine design for tuberculosis lead by Owen Leddy (everyone hiring new faculty in a few years, remember this name!) https://t.co/HzGB0OFrir

Does anyone have an in vitro T cell priming protocol they have successfully used in human PBMCs?

AbMAP, our work on adapting PLMs for antibodies, is now out in @PNASNews. A veritable zoo of PLMs now exists, so why should you care? Well, AbMAP reflects an opinionated design philosophy about antibody representation. If that design fits your need, it may be great for you! 1/

Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?

Please get in touch if this would be of interest to you! We are happy to host collaborators in our lab to learn how we do our MS analyses

We can also ask if peptides that you are excited about are presented by Mtb-infected cells.

We would love to collaborate with groups with HLA typed samples where we can interrogate the immunogenicity of these peptides.

We have the capacity to buy HLA typed blood and perform high sensitivity MS analysis to define Mtb-derived peptides on MHC-I and MHC-II.

TB friends! We are excited to expand our studies of MHC presentation of Mtb peptides to more alleles that capture human diversity. If you are involved in any ongoing human cohort studies where you are interested in studying T cell responses, we would love to collaborate!

Does anyone have favorite cell membrane/cytoplasm dyes for multi-day live cell imaging that are ideally 405-excitable and don’t induce appreciable phototoxicity?

There is still about a month to apply to our faculty position in #immunology and #immunoengineering @MITdeptofBE! We look forward to reading your applications

Anyone else see this paper? Perhaps a genome architecture explanation for NOS2 in mice v humans. https://t.co/7xMEIExzfe

TB friends! Anyone have a human CD4+ T cell clone that recognizes PPE18/Rv1196?

This approach has been truly indispensable for us. Rigorous biochemical validation of immunopeptidomics data is super important!

Anyone know where I can order milligram quantities of human MHC-II (with or without peptide?) NIH tetramer core is great but only does hundreds of micrograms.

Has anyone had success with targeting proteins to the lysosome using something other than LAMP sequences?