Selbach Lab
@SelbachLab
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Real flying ion Circus studying the proteome and its dynamics @BIMSB_MDC @MDC_Berlin
Berlin, Deutschland
Joined May 2017
MEK inhibition + growth factor → unexpected JNK activation 💥 Dual inhibition = significantly impaired tumor cell growth 🔬 SPIED-DIA = a powerful tool to dissect signaling & resistance #phosphoproteomics #cancerresearch #SPIED_DIA #MEKinhibition #DIA #precisionmedicine
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We present SPIED-DIA – a spike-in enhanced phosphoproteomics method combining DIA with targeted sensitivity to uncover synergistic signaling in colorectal cancer cells. 👉 [ https://t.co/R4nL8acLu8](https://t.co/R4nL8acLu8)
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What happens to all those alternative transcripts? 👉 Many are translated 👉 Proteolytic processing adds even more diversity Integrated mRNA & protein isoform landscape now out on bioRxiv! https://t.co/AazRU6WXgl
#proteomics #transcriptomics #systemsbiology
biorxiv.org
Alternative splicing and proteolytic processing expand the functional diversity of the human proteome by generating distinct protein isoforms from a single gene. However, the extent to which transc...
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Excited to share our new paper in MCP! 🎉 DIA with Spike-In SILAC (DIA-SiS) boosts proteome coverage and quantification. Check it out: https://t.co/cKBh5FjlgE
#Proteomics #MassSpec #Research
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Read our latest preprint on targeted phosphoproteomics: "Spike-in enhanced phosphoproteomics uncovers synergistic signaling responses to MEK inhibition in colon cancer cells.": https://t.co/UdzO3X4XQt
#Phosphoproteomics #DIA #CancerResearch #Signaling #ColonCancer
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Read the latest preprint on medRxiv: Cross-platform Clinical Proteomics using the Charite Open Standard for Plasma Proteomics (OSPP)
Want to do absolute quantitative plasma proteomics, with an internal standard that costs you less than 1€ per sample? Have a look at our OSPP
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Combining data independent acquisition with spike-in SILAC (DIA-SiS) improves proteome coverage and quantification. Read our new preprint on bioRxiv. #SILAC
#DIA
#Proteomics
https://t.co/DCNP2uE1Mr
biorxiv.org
Data Independent Acquisition (DIA) is increasingly preferred over Data Dependent Acquisition (DDA) due to its higher throughput and fewer missing values. Whereas DDA often utilizes stable isotope...
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Read our latest work on how phosphorylation in Intrinsically Disordered Regions (IDRs) affects protein-protein interactions in Nature Communications. @TRrustemi
https://t.co/qyZ54OJ5Bd
#IDR
#phosphorylation
nature.com
Nature Communications - Here the authors characterise the impact of phosphorylation site mutations in intrinsically disordered regions (IDRs) on protein-protein interactions, highlighting the...
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We would like to congratulate Fabian @CosciaLab for being awarded the European Research Council (ERC) Starting Grant 👏. #ERC
https://t.co/FLqlMHzTDs
mdc-berlin.de
Whether aggressive cancer cells can spread unchecked depends, to no small degree, on their cellular neighborhood. Fabian Coscia wants to analyze exactly what happens at the proteome level during this...
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Our latest preprint investigates the pathogenic mutations of human phosphorylation sites that affect protein-protein interactions. Using peptide-based interaction proteomics, more insights regarding the transcription factor GATAD1 were unveiled. @TRrustemi
biorxiv.org
Despite their lack of a defined 3D structure, intrinsically disordered regions (IDRs) of proteins play important biological roles. Many IDRs contain short linear motifs (SLiMs) that mediate protein...
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We completed a 5x5 km team relay yesterday. Some of us already look forward to running again next year.
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Interested in joining our Proteome Dynamics lab in Berlin? We are offering a postdoc position:
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Looking for a method to identify drug targets? Our new paper on drug target identification by quantitative proteomics is now online:
chemistry-europe.onlinelibrary.wiley.com
Parallel identification: We present a new compound interaction screen on a cellulose membrane (CISCM) that combines undirected photoimmobilization of compounds on cellulose with affinity purificati...
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How can you predict kinase activity in phosphoproteomic data? This database allows you to do this, even for poorly annotated kinases. Read more in this new paper: https://t.co/93O0Wnlcyu”
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Given the energy required for protein synthesis, degrading proteins seems to be wasteful. Read about the physiological functions of protein degradation in this new review:
annualreviews.org
While cellular proteins were initially thought to be stable, research over the last decades has firmly established that intracellular protein degradation is an active and highly regulated process:...
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